Altered Phase Interactions between Spontaneous Blood Pressure and Flow Fluctuations in Type 2 Diabetes Mellitus: Nonlinear Assessment of Cerebral Autoregulation

Cerebral autoregulation is an important mechanism that involves dilatation and constriction in arterioles to maintain relatively stable cerebral blood flow in response to changes of systemic blood pressure. Traditional assessments of autoregulation focus on the changes of cerebral blood flow velocity in response to large blood pressure fluctuations induced by interventions. This approach is not feasible for patients with impaired autoregulation or cardiovascular regulation. Here we propose a newly developed technique—the multimodal pressure-flow (MMPF) analysis, which assesses autoregulation by quantifying nonlinear phase interactions between spontaneous oscillations in blood pressure and flow velocity during resting conditions. We show that cerebral autoregulation in healthy subjects can be characterized by specific phase shifts between spontaneous blood pressure and flow velocity oscillations, and the phase shifts are significantly reduced in diabetic subjects. Smaller phase shifts between oscillations in the two variables indicate more passive dependence of blood flow velocity on blood pressure, thus suggesting impaired cerebral autoregulation. Moreover, the reduction of the phase shifts in diabetes is observed not only in previously-recognized effective region of cerebral autoregulation (<0.1 Hz), but also over the higher frequency range from ∼0.1 to 0.4 Hz. These findings indicate that type 2 diabetes mellitus alters cerebral blood flow regulation over a wide frequency range and that this alteration can be reliably assessed from spontaneous oscillations in blood pressure and blood flow velocity during resting conditions. We also show that the MMPF method has better performance than traditional approaches based on Fourier transform, and is more suitable for the quantification of nonlinear phase interactions between nonstationary biological signals such as blood pressure and blood flow.     

Phase control of Goos-Hänchen shifts in a four-level quantum dot nanostructure

In this letter, phase control of the Goos-Hänchen shifts of the reflected and transmitted probe light beams through a cavity containing four-level InGaN/GaN quantum dot nanostructure is theoretically discussed. In order to achieve the wave functions and their corresponding energy levels of the mentioned quantum dot nanostructure, Schrödinger and Poisson equations must be solved in a self consistent manner for carriers (here electron) in quantum dot. It is found that the coupling field, the pumping field as well as the cycling field can enhance the GH shifts of the reflected and transmitted probe beams. The effect of relative phase and the detuning of the probe light on the GH shifts of the reflected and transmitted probe beams are also investigated. We find that the GH shifts can be switched between the large positive and negative values by adjusting the controllable parameters.     

Coherent Control of the Goos-Hänchen Shifts in a Four-Level N Type Atomic Medium

The behavior of the Goos-Hänchen (GH) shifts of the reflected and transmitted probe light beams is theoretically investigated. In a fixed geometrical configuration, the effect of quantum interference induced by spontaneous emission on the phase control of the GH shifts is analyzed in this paper. It is found that in a four-level N-type atomic system as an intracavity medium, the GH shifts of the reflected and transmitted probe light beam are completely phase dependent.     

基于光纤光栅传感器的体外血管模型应力测量北大核心CSCD

血管中的血液流动会对血管壁产生周向应力,周向应力与血管的结构及功能变化密切相关,测量体外血管模型的周向应力是生物力学研究中较重要的问题。提出了利用光纤光栅测量血管周向应力的方法,基于微流控技术利用钢针模具建立了集成光纤光栅的三维圆形血管模型,通过仿真研究了不同流动速度与应力的关系。仿真结果得到流速在8 mm/s~75 mm/s范围内,应力随速度的改变呈线性变化。利用光纤光栅传感器测量了流体流动时产生的周向应力,根据实验得到了光栅波长改变量与速度的关系,流速范围在8 mm/s~75 mm/s之间变化时,速度引起波长的变化为0.173 nm。结合仿真实验得到了应力与光栅波长改变量的关系,为血液流动时产生周向应力的体外测量提供了新思路。     

一种局部相含率测量方法

在局部电导探针和单丝电容探针的基础上,提出一种新型局部电容探针,用于测量两相流的局部相含率。静态研究表明,局部电容探针能够测量空气水两相流的局部相含率,测量结果不受导电相电导率变化的影响。从工业蒸馏水变化到饱和食盐水(NaCl的质量分数0～5%),局部电容探针输出结果没有明显变化。电极距离在0～30 mm的范围内变化,测量结果变化小于1%。动态实验表明,局部电容探针测量的两相流的局部相含率,能够很好反映流动过程(以弹状流为例),和实验观察结果完全一致。只需要设置简单的阈值大小,就可以得到相含率的大小。     

基于光纤光栅的高灵敏度流速传感器

利用光纤光栅压强传感机构和汾丘里管设计了一种基于光纤光栅的流速传感器,并推导了光纤光栅中心波长漂移量与流速的关系式。实验表明,该传感器具有较高的灵敏度,稳定性较好,光纤光栅的中心波长随流速的增加而不断向短波方向漂移,而带宽几乎没有变化,实验和理论符合得较好。该流速传感器的动态感测范围为51.0~148.2 mm/s,在该范围内,至少可感测到0.3 mm/s的流速变化,这是目前所报道的最优值。优化光纤光栅压强传感机构及汾丘里管的参量,可测量其它速度段的流速,并可进一步提高传感灵敏度。     

57Co impurity-interstitial configurations in NbMo alloys observed by Mössbauer spectroscopy

Lattice defects in NbMo⁵⁷Co alloys produced by neutron irradiation at 4.6 K and by cold working were investigated by Mössbauer spectroscopy. On isochronal annealing up to about 100 K, trapping of two different types of mobile interstitials at the⁵⁷Co probe atoms was observed as additional lines in the spectra. These are characterized by centre shifts of +0.44 mm/s and +0.41 mm/s relative to⁵⁷Co on substitutional lattice sites and by large quadrupole splittings of 1.50 mm/s. Above 640 K, vacancy trapping was observed. With the onset of recrystallization, dislocation pinning was found in both the neutron irradiated and the cold worked sample.     

Wide dynamic range detection of bidirectional flow in Doppler optical coherence tomography using a two-dimensional Kasai estimator

We demonstrate extended axial flow velocity detection range in a time-domain Doppler optical coherence tomography (DOCT) system using a modified Kasai velocity estimator with computations in both the axial and transverse directions. For a DOCT system with an 8 kHz rapid-scanning optical delay line, bidirectional flow experiments showed a maximum detectable speed of >56 cm/s using the axial Kasai estimator without the occurrence of aliasing, while the transverse Kasai estimator preserved the approximately 7 microm/s minimum detectable velocity to slow flow. By using a combination of transverse Kasai and axial Kasai estimators, the velocity detection dynamic range was over 100 dB. Through a fiber-optic endoscopic catheter, in vivoM-mode transesophageal imaging of the pulsatile blood flow in rat aorta was demonstrated, for what is for the first time to our knowledge, with measured peak systolic blood flow velocity of >1 m/s, while maintaining good sensitivity to detect aortic wall motion at <2 mm/s, using this 2D Kasai technique.     

Tunable AC thermal anemometry

This paper suggests and demonstrates a novel flow measurement technique: tunable AC thermal anemometry, that allows simple integration, robust measurement and extremely high accuracy. The principle and simple theoretical analysis of the technique are presented. To find the optimal condition at which the phase lag becomes most sensitive to the flow speed change, the phase lag was measured scanning the heating frequency from 1 to 100 Hz, while the flow speed of ethanol was increased stepwise from 0 to 40 mm/s. The sensitivity of the phase lag depended on the heating frequency and the flow speed. It was possible to measure the average flow speed of 0.7 mm/s with the resolution of 0.1 mm/s at 4 Hz.     

Phase contrast MRI with improved temporal resolution by view sharing: k-space related velocity mapping properties.

Phase contrast techniques in combination with k-space segmented CINE imaging are widely used for the quantitative assessment of blood flow or tissue motion. The temporal resolution of the corresponding pulse sequences plays an important role concerning the potential of the method to fully detect time resolved flow or motion patterns. A further improvement of temporal or spatial resolution in phase contrast CINE MRI can be achieved by the application of view sharing. Based on simulations with point-spread-functions resulting from different cyclic flow or motion patterns an analysis of view sharing techniques in combination with phase contrast MRI is presented. Velocity mapping properties and the role of different k-space regions concerning the resulting values in the phase images and thus encoded velocities were investigated. It could be shown that the velocity induced phase shifts in phase contrast techniques are mainly encoded in the central sections of k-space which makes view sharing also suitable for velocity mapping. As a result the use of appropriate sampling and data acquisition schemes permits the assessment of flow or motion patterns with significantly improved temporal resolution without loss of functional information. In addition phantom measurements with an oscillation phantom were performed in order to validate the simulation results and to demonstrate the potential of view sharing techniques to accelerate phase contrast imaging and improve the detection of the underlying flow or motion dynamics.     

垂直管内油水两相流局部相分布特性实验研究

应用双头电导探针测量系统,对垂直上升管内油水两相分散流局部相分布特性进行了系统测量。得到了油水两相分散流的局部含油率分布类型图。研究结果表明低折算水速和低折算油速条件下,局部含油率在实验段截面上呈抛物线型局部分布特征,局部最大值出现在实验段中心区域。随折算油速增大,油滴受到横向力如升力的作用,逐渐向实验段壁面区域迁移,形成局部含油率的壁面峰值分布特性。当折算水速大于0．8 m／s时,局部含油率在实验段截面上呈均匀分布。     

CSF flow artifact reduction using cardiac cycle ordered phase-encoding method

The flow effects appear as a change of phase as well as signal intensity in NMR imaging. Since the flow of blood and CSF (cerebrospinal fluid) is pulsatile due to the heart pumping, their velocities are not constant during NMR imaging. This type of velocity fluctuation such as the blood or CSF flow induces irregular flow-dependent phase shifts, which have been one of the main causes of flow artifacts in NMR imaging. In order to reduce the flow artifacts, especially the CSF flow artifacts, a new cardiac cycle ordered phase encoding method is proposed and has been studied. This proposed technique utilizes the cardiac cycle as a precursor for the phase encoding gradients similar to the ROPE (respiratory ordered phase encoding) technique which has been used for respiratory motion artifact reduction. The basic concept and its applications are discussed together with the experimental results obtained with human volunteers using the KAIS 2.0 2.0 T whole-body NMR imaging system.     

激光微多普勒探测系统中降低相位噪音影响的方法研究

相干微多普勒激光雷达具有探测灵敏度高、探测信息量大等特点,特别适合于动目标探测、目标特征识别等应用.本文从线宽和探测距离两个方面讨论了模场相位随机起伏（相位噪音）对于测速准确度的影响,实验证实了相位噪音对激光微多普勒探测的影响,并探索出解决上述问题的方法——光纤补偿法.实验中,以输出波长为1.064 μm单块非平面环形腔激光器为光源,利用光纤补偿方法,并结合时频变换的算法,用外差探测的方式成功观测到了微多普勒频移,在传输距离为11 km时,系统最低探测速度达到了0.5 mm/s,速度分辨率达到了mm/s量级,频率分辨率达到了kHz量级,为微多普勒激光雷达的实际应用奠定了良好的实验基础.     

高效液相色谱-紫外检测法测定食品中的过氧化氢

通过在60℃下超声提取,用亚铁氰化钾和乙酸锌沉淀蛋白质,通过0.45μm滤膜净化样品。利用水和乙腈在200nm波长紫外吸收弱的特点,降低基线噪音。在0.5mL/min流速下,色谱柱对过氧化氢的保留增大,可以与杂质较好分离。用反相高效液相色谱-紫外检测色谱法,在C18柱上不经过衍生化测定了食品中过氧化氢的残留。过氧化氢的检出限为1.5×10^-7mol/L。对实际样品进行检测时,平均回收率R≥75%,RSD≤3%。     

Quantification of slow flow using FAIR

Phase contrast (PC)-based MRI methods are considered to be the most accurate approach for spatially resolved flow quantification, but the measurement of very slow velocities requires signal detection at long echo times and the application of strong field gradients. On the other hand, measurements based on time-of-flight or inflow effects can be conducted at short echo times and without flow-encoding gradients. A method for imaging flow at velocities of the order of 0.1 mm/s is presented and validated here. It consists of measuring the apparent spin-lattice relation rate (R₁*) of the flowing fluid using magnetization preparation by alternating slice-selective and nonselective inversion pulses (FAIR or flow-sensitive alternating inversion recovery) and a fast gradient-echo detection sequence. This method is appropriate for the quantitative imaging of slow flow at low Reynolds numbers in fluids where the T₂ values are too short to allow sensitive flow measurements by phase contrast-based methods.     

Full range spectral domain optical coherence tomography using a fiber-optic probe as a self-phase shifter

We present a full range handheld probe type spectral domain optical coherence tomography (SD-OCT) method. Here, the sample arm is composed of a tilted fiber-optic cantilever scanner; thus, the phase shift concurrently occurs while sample scanning. With the phase shift, we could achieve a full range complex-conjugate-free OCT image with no additional phase shifters in the reference arm. To realize this technique, a magnetically actuated probe was adopted. Full range SD-OCT images of a pearl, human fingernail, and human tooth were subsequently obtained using this suggested probe. The scanning range and acquisition speed were 3?mm and 20 frames/s?, respectively.     

Label-free optical biosensing with slot-waveguides

We demonstrate label-free molecule detection by using an integrated biosensor based on a Si(3)N(4)/SiO(2) slot-waveguide microring resonator. Bovine serum albumin (BSA) and anti-BSA molecular binding events on the sensor surface are monitored through the measurement of resonant wavelength shifts with varying biomolecule concentrations. The biosensor exhibited sensitivities of 1.8 and 3.2 nm/(ng/mm(2)) for the detection of anti-BSA and BSA, respectively. The estimated detection limits are 28 and 16 pg/mm(2) for anti-BSA and BSA, respectively, limited by wavelength resolution.     

Temporal interference effects by a pair of two-frequency laser beams — application to extremely low velocity measurements

A new proposal is described for the detection of low Doppler-frequency shifts or small displacements. The temporal interference term is expressed in terms of a beat-frequency wave with no phase term. Its amplitude is the same as the intensity formula produced by two equal-frequency laser beams. The significant low Doppler-frequency shifts included in this intensity formula, usually imbeded in the unwanted low frequency noise-spectra, becomes detectable from the amplitude of the beat-frequency wave. An application to a cross-beam Doppler velocimetry is successful for measurements down to the Doppler shifts as low as 0.03 Hz, corresponding to the velocity of 0.1 μm/s.     

HPLC-DAD法测定辣椒及其制品中苏丹红的含量

建立了应用高效液相色谱测定辣椒粉及其少量含油制品中的苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ含量的检测方法.采用Symmetry C18反相色谱柱(3.9mm×150mm,5μm),流动相为乙腈醋酸溶液(V乙磷:Vo.1%醋酸＝7∶1),等度洗脱,流速1.0mL/min;柱温30℃;检测波长230nm.在选定色谱条件下,苏丹红Ⅰ、Ⅱ、Ⅲ、...     

Behavior of the Melt in the Vertical Bridgman Method with a Low Axial Temperature Gradient

The thermal conditions for the growth of Ge crystals with a diameter of 50 mm by the vertical Bridgman method in the case of low thermal-gravitational convection are studied using model experiments. Distilled water being hydrodynamically similar to the Ge melt is used as the model liquid. When modelling by means of the light cut method, it is established that mixture particles move along the heat flow direction from top to bottom. It is shown that an axial temperature gradient of 2 K/cm or more increases the contribution of thermal diffusion to mass transfer at a vertical flow rate of 0.09 mm/s or more. The numerical simulation of thermal convection in the Oberbeck–Boussinesq approximation confirms the absence of convection under the given thermal conditions. However, the deviation of the container axis from the vertical by 0.5° during the process of crystal growth contributes to the increase in the flow rates in the liquid phase up to 0.55 mm/s.