激光微束辐照剂量对细胞存活率外源物质导入率影响的研究

本文介绍了一套经过改进的Nd:YAG紫外激光微束系统,以小鼠巨噬细胞及Hela细胞为实验材料,并利用多种荧光物质作为导入物,系统地研究了激光微束辐射剂量与细胞存活率、外源物质导入率的关系,为深入地研究激光微束与细胞的相互作用机理摸索出优化条件.     

飞秒激光脉冲的高效率放大研究

 介绍了采用多通放大的飞秒脉冲掺钛蓝宝石激光放大系统,分析讨论了提高放大效率及缩短脉冲宽度的技术方法。在仅用能量290mJ的倍频Nd:YAG激光泵浦下,得到了输出峰值功率大于1.4TW、脉宽25fs、重复频率10Hz的超短超强激光脉冲。     

锁模激光脉冲串放大过程中的整形控制

 为了获得具有特殊要求的驱动激光,设计了一套以Nd:YVO4锁模激光器为种子光,用二极管激光器泵浦的Nd:YAG放大系统进行放大的激光系统。对放大过程中锁模激光脉冲串因增益饱和造成的脉冲串包络畸变以及对包络的整形控制进行了研究。实验获得能量为24 mJ的基频光,5.7 mJ的倍频光,0.608 mJ的紫外四倍频光;通过脉冲串整形控制,实现了包络顶部平整的紫外超短脉冲串输出,满足了光阴极注入器对驱动激光的特殊需求。     

激光应用 激光生物学

Q631 2005042602 脉冲Nd:YAG激光对单层KB细胞损伤效应的研究= Effect of single pulse Nd:YAG laser irradiation on mono- layer KB cell cultures[刊,英]／陈虹霞(军事医学科学院放射医学研究所,北京(100850)),杨在富…／／激光生物学报,-2005,14(1),-12-16 观察了脉冲Nd:YAG激光照射体外单层培养KB细胞后的形态改变及损伤后KSP70,c-Fos的表达情况,初步     

Nd:YAG调Q激光器下能级寿命对激光脉冲性能的影响

在对Nd:YAG调Q激光器进行分析和设计时,研究人员通常选择忽略激光下能级寿命对脉冲波形的影响。当激光脉宽远大于激光下能级寿命时,这种近似一般不会带来太大偏差;而当脉宽达到纳秒量级时,Nd:YAG晶体约30 ns的下能级寿命对脉冲波形的影响会变得非常严重。建立了Nd:YAG下能级寿命对输出脉冲波形影响的理论分析模型,并对窄脉宽的Nd:YAG调Q激光器的输出波形进行仿真研究。研究结果表明,在窄脉宽激光输出情形下,激光下能级寿命会导致调Q脉冲在主峰后出现尾峰,尾峰能量可达主峰能量的一倍以上。同时建立了Nd:YAG声光调Q激光器实验系统,在与仿真计算近似的条件下测量调Q脉冲波形,观察到与仿真结果一致的尾峰现象,实验验证了理论模型的正确性。     

飞秒与皮秒激光脉冲的主动同步及和频产生宽带超短激光的研究

利用主动同步方式实现了飞秒钛宝石激光器与皮秒Nd:YVO₄激光器的同步，得到了时间抖动低于1ps的同步精度.在此基础上进行了两束激光的和频实验研究，产生了波长为460nm的宽带蓝光飞秒脉冲激光.这一技术不仅代表了实现不同波长、不同脉冲宽度激光同步的一般方法，而且也证明了通过和频、差频两束不同激光产生新波长超短脉冲激光的一种全新技术方案. 关键词： 主动同步 超短脉冲 和频 差频     

半导体可饱和吸收镜被动锁模侧面抽运Nd:YAG激光器研究

利用半导体可饱和吸收镜(SESAM)锁模技术实现的超快脉冲激光器具有结构简单紧凑、脉冲序列稳定等优势,在许多领域有着重要用途,自问世以来受到国内外的广泛关注.分析了SESAM被动锁模侧面抽运固体激光器的具体要求,进行了不同条件下的SESAM被动锁模侧面抽运Nd:YAG激光器实验.获得了最高平均功率9.5 W,脉冲重复频率71 MHz,单脉冲能量约141 nJ的皮秒激光脉冲.对SESAM被动锁模侧面抽运Nd:YAG激光器进行了实验和理论分析,对实现高功率连续超短脉冲激光器进行了探讨.     

超短脉冲激光照射下光栅Talbot效应的实验研究

理论上研究了超短脉冲激光照射下光栅的Talbot效应,得到了超短脉冲激光照射下光栅Talbot图像的性质.实验上采用严格的实验方法(频率分辨光学开关装置测量超短脉冲激光、反射式扩束系统对超短脉冲进行扩束)很好地验证了理论分析结果.理论分析和实验结果表明,超短脉冲激光照射下光栅的Talbot图像的对比度会显著下降,而且超短脉冲激光的脉宽越短,Talbot距离越大,Talbot图像的对比度会进一步下降.     

有热配合的激光/前视红外测距机

本发明论及一个由前视红外(FLIR)系统和激光系统组成的设备,前视红外系统用于目标定位和识别,激光系统用于测定目标距离。过去,典型的测距仪系统有一个相干辐射源,如Nd:YAG激光器,它所产生的脉冲能量发射到目标上,经目标反射,然后在发射点接收。整个传输时间可用来测量目标距离。测距机一般是用热成象传感器,例如前视红外,提供的信息来对准目标。以前的装置存在的问题很多,尤其是在恶劣气侯下,Nd:YAG测距机发射的能量     

基于激光超声的微裂纹检测技术的研究

在固体中利用激光产生超声波 ,可作为超声测量和材料无损检测的一种新方法。介绍了激光超声表面波的产生机理、微裂纹的检测方法及其应用。采用Nd∶YAG脉冲激光器、扩束与聚焦透镜组、PZT探头、数据分析仪等器件设计并构建了一套基于接触式检测方法的激光超声微裂纹检测实验系统。通过对大量实验数据进行处理 ,得出了相应的各种关系曲线 ,说明了线光源产生的超声表面波非常适用于材料表面微裂纹的检测     

A novel method of DNA transfection by laser microbeam cell surgery

A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude.     

光纤光栅可调谐半导体单模超短光脉冲的研究

本文利用光纤光栅形成外腔反馈,得到量子阱半导体激光二极管单模超短光脉冲.其光谱线宽约为0.4nm,脉冲宽度约为60ps.并利用调谐装置,改变光纤光栅的反射波长,得到不同波长的超短光脉冲,其波长可长期稳定.     

二极管面阵侧泵浦Nd:YAG双板条激光器

 叙述了二极管面阵侧泵浦双板条耦合单横模 Q 开关激光器的研制。泵浦源为二极管面阵激光器,使用梯形波导板耦合系统,耦合效率达90%,谐振腔为凸凹腔,全反射镜的曲率半径为 3.0m的凹面镜,输出镜为曲率半径3.0m的可变反射率超高斯镜。激光器在 100Hz 重复频率下,输出单脉冲能量18.23mJ,脉宽为15.25ns,光束质量M²<1.5具有平顶分布的超高斯光束。     

Calculation of the Spatio-Temporal Dynamics of Q-switched Yb^3＋：YAG Laser with an Unstable Cavity and a Super-Gaussian Mirror

A numerical simulation used to compute the spatio-temporal dynamics of pulse formation of diode-pumped Q- switched Yb： YA G laser is carried out. The model takes the laser amplification and gain saturation, the properties of the laser cavity, and the diffractive effects of the laser disc into account. The numerical calculation is performed for a confocal positive-branch unstable resonator with a super Gaussian coupling mirror. The simulation results show that the laser pulse starts from a Gaussian intensity distribution and becomes rapidly non-Gaussian. The corresponding beam quality M^2 factor is seen to vary approximately from 1.5 at the beginning of the formation of pulse to more than 10 in the tail of the pulse, with a value of 11.6 at the peak of the pulse.     

A self-quenching picosecond distributed feedback dye laser pumped by a picosecond laser pulse

A tunable single short pulse laser system with a transform–limited bandwidth pumped by a picosecond Nd:YAG laser (ca. 120 ps pulse width) is demonstrated. With this configuration, the relaxation oscillations coming from a distributed feedback dye laser cavity are completely removed. Because the pumping pulsewidth is shorter than the lifetime of dye molecule on the upper laser state, the gain of the laser medium is depleted by the first pulse. The laser wavelength could be precisely tuned with the transform-limited bandwidth (for example, a linewidth of 0.02 nm). After amplification, we obtain a single short laser pulse energy up to 500 μJ at the pulsewidth of 8.2 ps.     

High-energy single longitudinal mode 1 ns all-solid-state 266 nm lasers

A single longitudinal mode (SLM) short pulse high energy all-solid-state ultraviolet laser is demonstrated in this paper. Through the use of a master oscillator power amplifier (MOPA) architecture, we have been able to provide high-energy outputs with the combination of short pulses, good beam quality and SLM typically produced by a 1064 nm Nd:YAG laser. The passively Q-switched SLM Nd:YAG laser in a twisted-mode cavity is operated as the seed source. After the seed is amplified by a double-pass pre-amplifier and a single-pass main-amplifier, the 100 μJ, 1064 nm, ∼1 ns seeding pulse was amplified up to 400 mJ energy in the total pulse train. Using a KTP crystal for second-harmonic generation and a CLBO for fourth-harmonic generation, we successfully obtained a short pulse, high energy ultraviolet laser of 266 nm, with the output energy of 108 mJ, pulse width 1 ns and M²<5. PACS 42.65.Ky; 42.72.Bj; 42.60.Da     

Influence of consecutive picosecond pulses at 532 nm wavelength on laser ablation of human teeth

The interaction of 40 ps pulse duration laser emitting at 532 nm wavelength with human dental tissue (enamel, dentin, and dentin–enamel junction) has been investigated. The crater profile and the surface morphology have been studied by using a confocal auto-fluorescence microscope (working in reflection mode) and a scanning electron microscope. Crater profile and crater morphology were studied after applying consecutive laser pulses and it was found that the ablation depth increases with the number of consecutive pulses, leaving the crater diameter unchanged. We found that the thermal damage is reduced by using short duration laser pulses, which implies an increased retention of restorative material. We observe carbonization of the irradiated samples, which does not imply changes in the chemical composition. Finally, the use of 40 ps pulse duration laser may become a state of art in conservative dentistry.     

采用多通腔望远镜谐振腔结构的10MHz重复频率飞秒钛宝石激光器特性研究

通过在钛宝石激光腔内引入望远镜长腔系统增加腔长,实现了重复频率低到10?MHz的飞秒激光振荡,在5?W的抽运功率下获得了平均输出功率200?mW、单脉冲能量20?nJ的稳定输出. 在此基础上分析了腔内不同色散情况下的输出脉冲光谱和脉宽特性,结果表明在腔内存在一定负色散的情况下,锁模脉宽可接近转换极限,最短脉宽可达56?fs. 而在正色散的情况下,锁模输出的脉宽较宽,并且随着腔内正色散的增多,脉宽可到大于600?fs,锁模光谱也呈马鞍形. 关键词： 钛宝石激光器 飞秒脉冲 低重复频率 望远镜腔     

Laser-based transfection with conjugated gold nanoparticles

The irradiation of ceils combined with the immunoconjugate of gold nanoparticles by the short pulse laser can make the plasma membrane be transiently permeabilized, which can be used to transfer exogenous molecules into the cells. We explore this technique as a novel gene transfection method for floating cells. Three different floating cells exposed to the laser are selectively transfected with fluorescein isothiocyanatedextran, antibody, and green fluorescent protein （GFP） coding plasmids, and the viability of cells are determined by propidium iodide. For fluorescein isothiocyanate-dextran, the best transfection efficiency of 65% is obtained; for the antibody, it is 74%; whereas for the green fluorescent protein coding plasmids, a very small transfection efficiency is gained. If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.     

光谱增感剂对AgBr微晶光电子衰减时间特性的影响

本工作利用高时间分辨的微波吸收薄膜介电谱测量技术,测量了菁染料光谱增感后的AgBr晶体在35ps超短脉冲激光曝光后产生的自由光电子和浅俘获光电子随时间衰减的光电子衰减时间分辨谱,分析了不同的染料增感条件对材料光电子时间特性的影响关系,实验验证了吸附在T-颗粒(111)晶面上的染料比吸附在立方体(100)晶面上的染料更有效、更有助于形成潜影的论据。