12C6+离子束辐照番茄种子当代效应

利用辐射能量为80 MeV/u 的12C6+重离子束辐照番茄种子, 辐照剂量分别为30, 60, 90, 120和160 Gy研究其对番茄M1代的生物学效应。结果表明, 随着辐照剂量的增大, 番茄的发芽率和成苗率降低, 且成苗率明显低于发芽率, 发现辐照损伤主要抑制了根的生长; MDA和脯氨酸含量变化的总体趋势为随着辐照剂量的增大先升后降再升高, 说明高剂量C离子辐照对生物膜造成更严重的损伤; APX活性随着辐照剂量的增大呈先升后降再升高的趋势, 表明APX在清除活性氧中起主要作用; POD和SOD活性的总体趋势是随着辐照剂量的增大而降低, 且明显低于对照组。综合分析表明, 12C6+重离子束辐照番茄种子, 对M1代具有明显的损伤效应, 高剂量辐照对番茄种子造成的损伤更大, 使酶的活性降低, 抑制植株生长。 To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by 12C6+heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to 12C6+ heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.     

12C6+束辐照引起的实验猪皮肤急性损伤反应的研究

探讨了正常皮肤对重离子辐照急性损伤反应的耐受性, 为重离子治癌临床应用提供安全性检测的实验依据。实验前10 min, 实验猪肌肉注射复方氯胺酮1.2 mg/kg进行麻醉, 然后在兰州重离子研究装置（HIRFL）辐照终端利用12C6+束照射, 辐照剂量分别为0, 12, 21和27 Gy, 辐照分3次完成, 剂量率约为1.2 Gy/min, Bragg峰区照射, 辐照后每隔7 d对照射野拍照并活检取样, 做HE组织病理学观察。不同剂量12C6+离子束辐照实验猪皮肤后, 皮肤外观反应随辐照剂量增大而加快, 表现为肿胀和色素沉积等; 皮肤组织结构的变化明显, 上皮细胞排列紊乱、 萎缩、 空泡变性; 基本恢复正常所需时间也越长, 且都存在明显的剂量效应关系。结果表明, 辐照剂量范围为0—27 Gy时, 重离子对正常皮肤的辐照是安全的。The tolerance of the normal skin to the acute radiation injury reaction induced by heavy ion beams has been studied experimentally. The experimental pigs were injected with 1.2 mg/kg ketamine in 10 min before irradiation and were irradiated with 0, 12 , 21 and 27 Gy 12C6+ ion at a dose rate of 1.2 Gy/min at the Heavy Ion Research Facility in Lanzhou（HIRFL）. The total radiation dose was finished by 3 times at Bragg Peak Region of Heavy Ion Beams.The radiation fields of skin were taken photo and performed biopsy. The contaneous tissues of radiation fields were stained by HE and examined histopatholo gical changes every seven days after irradiation. The results indicated that the cutaneous appearance reaction became more faster with radiation dosage rising and presented with swollen, melanin forming and so on after irradiated by the carbon ions at different dosage. The Pathological examination showed noticeable changes in histological and structural of experimental pigs skin, such as atrophy, vacuolation, denaturation and arranged irregularly in epithelial cells. Furthermore, the time for return to normality became longer with the increasing of radiation dosage. All indexes demonstrated correlation between the does and effects. It is concluded that the irradiation of heavy ion beams to normal skin is security when the radiation dose range is about 0—27 Gy.     

二烯丙基二硫对12C6+离子束辐照损伤小鼠的保护作用研究

研究大蒜素重要活性成分二烯丙基二硫( Diallyl disulfide, 简称DADS) 对12C6+离子束辐照损伤小鼠的保护作用。利用4 Gy 剂量12C6+离子束对不同浓度DADS 预处理的雄性昆明小鼠进行单次全身照射。随后检测骨髓细胞微核率和肝组织中丙二醛(MDA) 含量、蛋白质总羰基含量、总抗氧化能力( TAOC)及谷丙转氨酶(ALT ) 活性。结果显示,与单纯照射组相比,低浓度DADS 预处理组骨髓细胞微核率和肝组织ALT 活性均显著降低(p<0.001),而肝组织T-AOC明显增强( p < 0.05 );中浓度DADS 预处理组肝组织中MDA 含量和蛋白质总羰基含量均显著减少( p < 0.05 )。结果提示,DADS通过抑制氧化应激,有效地保护了脂质、蛋白质和遗传物质免受12C6+离子束辐照引起的损伤。The radioprotective effect of Diallyl disulfide (DADS) on 12C6+ ion irradiation was studied. Pretreated with DADS of different concentration, male Kung-Ming mice were exposed to whole body irradiation with dosage of 4 Gy 12C6+ ion. The animals were sacrificed after irradiation. Then the bone marrow cells micronucleus rate, malondialdehyde (MDA) levels, content of protein carbonylation, total antioxidant capacity ( T-AOC) and alanine aminotransferase ( ALT ) activity were measured. As compared with those in irradiated group, the ratio of micronucleus cells in marrow and the hepatic ALT activity in the pretreatment group with low dose DADS decreased significantly ( p < 0.001 ). Similarly,the content of protein carbonylation and the levels of MDA droped dramatically in the group with middle dose DADS treatment ( p < 0.05 ). On the contrary, the hepatic T-AOC increased markedly in the group of pretreatment with low dose DADS ( p < 0.05 ). The results showed that DADS protect lipoid, protein and genetic material from 12C6+ ion irradiation by right of resisting oxidative stress.     

12C6+离子对阿维链霉菌累进辐照诱变效应研究

选用12C6+ 离子束对阿维链霉菌诱变选育高产菌株与原始菌株进行辐照诱变, 研究其累进辐照效应。实验结果表明,在辐照剂量为10 Gy时, 原始菌株比诱变高产菌株存活率高, 抗辐射能力强;辐照剂量高于30 Gy时,诱变高产菌株比原始菌株存活率高, 抗辐射能力强。原始菌株正突变率最高的辐照剂量为50 Gy, 致死率99.43%,正突变率最高, 达34.2%;对诱变高产菌株辐照剂量为30 Gy,致死率94.97%,正突变率最高, 达23.5% 。累进辐照效应降低了最佳辐照剂量。 Mutagenic effect on the mutant high producing strain ZJAV Y1 203 and the original strain ZJAV A1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant high producing Strain at dose of 10 Gy. The mutant high producing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAV Y1 203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.     

不同剂量重离子辐照玉米自交系的生物学效应比较

用12C6+和 36Ar18+离子束分别辐照玉米自交系干种子和浸泡种子, 研究了M1—M3代重离子束辐照的生物学效应。 结果表明: 种子发芽势和发芽率随辐照剂量的增加而下降, 不同生理状态的种子对重离子辐照的敏感性也不同。 一般12C6+ 离子辐照干种子的适宜剂量为20—25 Gy; M1代叶型发生明显的变化, M2代植株在株高、穗位、单株穗数、雄穗花药颜色、粒质、穗行数、粒重和抗性等方面均发生了变化, 并产生了许多有益的变异,包括株高和穗位降低、同位多穗、穗行数和粒重增加、粒质由粉质变为硬粒以及抗锈病和红叶病的植株等, 有益突变的频率达7.0％—17.9％;在M3代出现能够稳定遗传的,并且光合效率增加的有益突变株。由此可见,重离子束辐照是玉米种质改良的一种高效手段。 In order to study biological effects of heavy ion irradiation on maize inbred lines, the agronomic traits and photosynthetic rates were investigated from M1 to M3 of maize seeds irradiated with 12C6+ and 36Ar18+ ions．The results showed that the germination rate and planting percent of maize seeds irradiated were decrease as dosage increasing of heavy ion irradiation. Different physiological status of seeds had disparate sensibility to heavy-ion irradiation and the suitable dosage of 12C6+ ion irradiation was 20—25 Gy for dry maize seeds. The leaf type of the plant happened visible changes in M1 generation. The plant height, spike position, spike number per plant, anther color of staminate,grain texture,spike row,grain weight and resistance had changes in M2 generation. Among them occurred some beneficial mutations that include degrading of plant height and spike position height, multi spike at same position in the plant, increasing of pike row and grain change of grain texture from powder seed to hard seed,resistance to rust disease and red leaf disease and so on. The frequency of beneficial mutation was 7.0％—17.9％. Those beneficial mutations could be stably inherited and mutant plants with high photosynthetic efficiency emerged in M3 generation. The study above showed that heavy ion irradiation is a high performance means for improvement germplasm of maize.     

12C6+ 离子束辐照对大鼠离体主动脉环内皮依赖性舒张功能的影响

利用兰州重离子研究装置(HIRFL) 提供的高能12C6+离子束(能量为300 MeV/u,剂量率为0.5 Gy/min) 辐照大鼠离体胸主动脉环,考察了12C6+离子束辐照对主动脉环内皮依赖性舒张功能的影响,并采用NBT 还原法测定血管环生成超氧阴离子(O2􀀀) 水平,加入外源性超氧化物歧化酶(SOD) 干预探讨了O2􀀀在内皮功能损伤中的作用。研究结果表明,2.0,4.0 和6.0 Gy 的12C6+离子束辐照大鼠胸主动脉环后,可致血管内皮依赖性舒张功能的剂量依赖性明显受损(P <0.01 vs control group),并可致血管环NBT 还原能力剂量依赖性增加(4.0 Gy 时,P <0.05;6.0 Gy 时,P <0.01 vs control group)。辐照前加入外源性SOD 对6.0 Gy 12C6+离子束辐照所致血管环NBT 还原能力升高有明显抑制作用(P <0.01),对血管环内皮依赖性舒张功能也有明显的保护作用(P<0.01),但辐照后10 min 加入外源性SOD,其保护作用明显不及前者。结论显示,12C6+ 离子束辐照大鼠胸主动脉环可致血管内皮功能受损,O2 清除剂SOD 对内皮功能受损有保护作用,说明O2􀀀介导了辐照所致内皮功能损伤。Heavy ion beam has many characteristics,and it is expected to be the most suitable radiation therapy technique for malignant tumor. It is lack of depth-understanding on the potential adverse reactions caused by using this technique, because heavy ion radiotherapy is applied to clinical for a short time. Studies have shown that the vascular injury plays a pivotal role in normal tissue damage induced in the conventional radiation therapy, but there was no research report on heavy ion beam irradiation-induced vascular injury. In the present study, the isolated aortic rings of rats were irradiated by 12C6+ ion beam (300 MeV/u, 0.5 Gy/min) delivered by HIRFL(Heavy Ion Research Facility in Lanzhou), the effects of 12C6+ ion beam irradiation on aortic rings with endothelium dependent diastolic function have been investigated.NBT reduction method was used for assaying the vascular ring formation of superoxide anion (O2􀀀) level, and the involvement of superoxide anion in endothelial function injury in rats was investigated through the intervention test of exogenous superoxide dismutase (SOD) on O2. The results showed that, the vascular endothelial dependent vasodilation was impaired significantly (P < 0:01 vs control group) by irradiation with 2.0, 4.0 and 6.0 Gy 12C6+ ion beam in a dosedependent manner, and the NBT reduction of vascular rings increased dose-dependently (P <0.05 at 4.0 Gy, P <0.01 at 6.0 Gy vs control group). Adding exogenous SOD before irradiation could significantly inhibit the increasing of NBT reduction (P<0.01), and also had protective effect on vascular endothelium dependent diastolic function (P<0.01), but 10 min after irradiation with exogenous SOD, its protective function was significantly less than before. Conclusion indicated that 12C6+ ion beam irradiation could cause endothelial function impaired, O2􀀀 scavenger SOD has a protective effect on endothelial dysfunction, suggesting that O2􀀀 mediates endothelial injury induced by heavy ion irradiation.     

12C6+离子辐照对4种农作物种子出苗率

以能量为80 MeV/u的12C6+离子为诱变源辐照油菜、 胡麻、 大葱和兵豆的干种子后, 研究了不同剂量处理对4种农作物M1和M2代种子出苗率及幼苗生长的影响。 实验结果表明: 重离子所导致的M1代生物学效应因不同的物种而表现出一定的差异, 适当剂量C离子辐照促进了油菜和胡麻M1代出苗率和幼苗的生长; 而不同剂量的C离子辐照抑制了大葱的出苗率和幼苗的生长; 兵豆3个剂量下的出苗率和对照相差很小, 但90 Gy辐照有利于其生长。 到了M2代, 4种作物辐照组的发芽率都低于各自的对照组; 30 Gy剂量下的油菜、 胡麻和兵豆长势最好; 大葱依然是对照的长势最好。 Crops of Brassica napus L., Linum usitatissmum L., Allium fistulosum L. and Lens culinaris Medic. were irradiated by 80 MeV/u 12C6+ ion beams with doses of 30, 90 and 180 Gy. The germination rates and heights of seedlings of M1 and M2 generation of these four plants were studied. The results indicated that germination rates and average heights of the B. napus and L. usitatissmum were improved by appropriate dose treatment, while great suppression was found in the irradiated groups of the A. fistulosum. As far as the L. Culinaris was concerned, little differences was observed on M1 germination rate, but the 90 Gy irradiation was favorable to growth of plant. The treatments with 30, 90 and 180 Gy were inferior to contrast one on M2 germination rate of the four species. Seedlings of M2 generation of the B. napus, L. sitatissmum and L. culinaris under 30 Gy grew better than the other groups, while the best performance of the A. fistulosum was shown by the control group.     

羧甲基-β-1,3-葡聚糖对人肝癌HepG2细胞的放射增敏作用

本研究旨在探讨羧甲基-β-1,3葡聚糖（CMG）对人肝癌HepG2细胞X射线或12C6+离子束辐射敏感性的影响。首先用CCK-8法检测CMG对HepG2细胞的生长抑制情况,得到半数抑制浓度（IC50）为120.6μg/mL。用浓度为0.1×IC50的CMG预处理HepG2细胞24 h,再给予2 Gy X射线或12C6+离子束辐照（CMG+辐照组）;CMG未处理组直接接受2 Gy X射线或12C6+离子束辐照（辐照组）。对比分析辐照组和CMG+辐照组细胞的克隆存活、DNA损伤、凋亡与周期分布、细胞内活性氧（ROS）水平。发现:与X射线辐照组相比,相同剂量的12C6+离子辐照组克隆存活率更小,DNA损伤和周期阻滞更加严重,细胞凋亡率和细胞内ROS水平也更高。与单独X射线或12C6+离子束辐照组相比,CMG+辐照组克隆存活率明显降低,细胞凋亡率随辐照后CMG作用时间的延长而明显增加,CMG使辐照后细胞内ROS维持在一个较高的水平,同时CMG明显加重了单独辐照诱导的DNA损伤和周期阻滞。结果表明,与X射线相比,HepG2细胞对相同剂量的12C6+离子辐射更敏感;CMG可增加HepG2细胞对X射线或12C6+离子辐射的敏感性;CMG可能通过增加受照HepG2细胞内的ROS水平,加剧辐照诱导的DNA损伤,促进辐射诱导细胞凋亡而起到辐射增敏作用。This study aims to investigate the effect of carboxymethy-β-1, 3-glucan (CMG) on the sensitivity of human hepatoma HepG2 cells to X-rays or 12C6+ ions irradiation. First, the inhibitory effect of CMG on the growth of HepG2 cells was detected by CCK-8 assay, and the half maximal inhibitory concentration (IC50) was 120.6 μg/mL. HepG2 cells were pretreated with CMG at a concentration of 0.1×IC50 for 24 h and then irradiated with 2 Gy X-ray or 12C6+ ion beams (CMG + irradiation group). CMG untreated group was directly irradiated by 2 Gy X-rays or 12C6+ ions beam (irradiation group). The clone survival, DNA damage, cell apoptosis, cell cycle distribution, and intracellular reactive oxygen species (ROS) levels in irradiation group and CMG + irradiation group were comparatively analyzed. The results showed that the clone survival rate was lower, DNA damage and cycle arrest were more serious, and the rate of apoptosis and intracellular ROS levels were higher in 12C6+ ions irradiation group than those in the same dose of X-rays irradiation group. Compared with X-rays or 12C6+ ions irradiation group, the clone survival rate of CMG + irradiation group was significantly decreased, and the apoptosis rate significantly increased with the prolongation of CMG treatment post-irradiation; CMG maintained intracellular ROS at a higher level after irradiation, CMG also significantly aggravated radiation-induced DNA damage and cycle arrest. These results indicated that HepG2 cells were more sensitive to 12C6+ ions radiation than those at the same dose of X-rays. CMG increased the sensitivity of HepG2 cells to X-rays or 12C6+ ions irradiation by increasing intracellular ROS level, exacerbating radiation-induced DNA damage and promoting radiation-induced apoptosis in irradiated HepG2 cells.     

不同LET碳离子束辐照拟南芥干种子的当代损伤效应

本研究以拟南芥（Columbia野生型）干种子为材料,利用兰州重离子研究装置（HIRFL）产生的碳离子束对材料进行辐射处理,统计其存活率、根长、下胚轴长及每果荚种子数,以探讨不同传能线密度（Linear Energy Transfer,LET）的碳离子束辐照对拟南芥当代损伤效应的影响。结果表明,在相同LET辐射条件下,随着辐射剂量的增大,拟南芥的存活率、根长、下胚轴长度、每果荚种子数都呈现下降趋势。在相同剂量不同LET辐射处理情况下,随着LET的增大,存活率、根长、下胚轴长、每果荚种子数都显著下降,可见高LET辐射严重抑制了拟南芥的生长和发育。研究表明,当LET为50 keV/μm时,碳离子束辐射拟南芥干种子对应的最佳诱变剂量为200 Gy,为后续开展碳离子束辐射的诱变效率研究奠定了前期基础。Aimed to study the biological effects of carbon ion beams with different linear energy transfer (LET) values provided by Heavy Ion Research Facility in Lanzhou (HIRFL), dry seeds of Arabidopsis thaliana (Columbia-WT) were irradiated and a series of biological effects of postembryonic development, such as survival rate, primary root length, hypocotyls length and number of seeds per silique, were investigated. The results showed that, under the radiation condition of the same LET value, the survival rate, root length, hypocotyls length and number of seeds per silique were decreased with the increasing dose. In addition, under the radiation conditions with different LET values, but same dose, the extent of the decline of the survival rate, root length, hypocotyls length and number of seeds per silique were reinforced with the increasing LET. It was also found that high LET radiations inhibited the subsequent growth and development of Arabidopsis thaliana severely. In brief, it was suggested that the optimum dose of carbon ion beam with 50 keV/μm value on Arabidopsis thaliana dry seeds was 200 Gy. This research complemented the preliminary theoretical foundation for the comparative study of the highest mutation efficiency of carbon ion beam irradiations at IMP, CAS(Institute of Modern Physics, Chinese Academy of Sciences).     

重离子束对北里链霉菌的诱变效应

考察不同剂量重离子束对北里链霉菌的致死及诱变效应, 可确定最有利于筛选高产菌株的重离子束辐照剂量。 利用不同剂量的重离子束辐照北里链霉菌孢子, 统计了存活率、 致死率、 正负突变率。 结果发现, 在5 Gy重离子辐照时北里链霉菌出现较高致死率, 其后随剂量升高, 致死率变化较平缓。 各辐照剂量下正负突变率相比较, 40 Gy时正突变率最高, 负突变率相对较低, 存活率为0.92%。 因此确定40 Gy是对北里链霉菌高产菌株筛选最有利的辐照剂量。 To define the optimum dose of heavy ion beams for selecting high productive strains, we should study mortality and mutation effects of Streptomyces kitasatoensis irradiated by heavy ion beams in diffe rent doses. In this research, spores of Streptomyces kitasatoensis were irradiated by heavy ion beams with different doses. And survival rate, mortality rate, positive mutation and negative mutation were analyzed statistically. The results showed that high mortality rate appeared from 5 Gy and then the mortality rate curve became gently. Compared the positive and negative mutations in different doses, highest positive mutation was obtained in 40 Gy, while the negative mutation was lower in this dose, and the survival rate was 0.92%. So we defined that optimum dose of heavy ions radiation for Streptomyces kitasatoensis selection was 40 Gy in this experiment.     

~(12)C~(6+)离子束辐照紫苏干种子当代效应

利用兰州重离子研究装置(HIRFL)提供的12C6+离子束辐照紫苏干种子(辐照剂量为40,80和120Gy,剂量率4Gy/min),探讨了重离子束辐照对紫苏M1代的生物学效应。结果发现,经不同剂量的12C6+离子束辐照后,紫苏种子的发芽率、发芽势、存活率、株高、分枝数、单株产量和千粒重等生物学性状均发生了变化,其中发芽势、单株产量和千粒重随辐照剂量的提高而降低,且有明显的剂量效应关系,但发芽率、大田成活率、株高和分枝数却随辐照剂量的增大,呈现出明显的"抛物线"趋势;紫苏幼苗根尖细胞的微核率和染色体畸变率随辐照剂量增加呈线性增加关系。这表明:12C6+重离子束辐照紫苏种子,具有明显的当代损伤效应,在本试验剂量范围内,低剂量辐照对发芽率和成活率有促进作用。     

12C6+离子束辐照黄花蒿干种子当代生物学效应

利用12C6+重离子束对黄花蒿干种子辐照后,利用发芽率、根长、下胚轴长和株高4个指标研究不同剂量12C6+离子束辐照对黄花蒿的生物学效应。结果表明,随着辐照剂量的增加,黄花蒿的存活率和根长都呈现逐渐降低的趋势,而下胚轴长先上升后下降,株高总体呈现下降的趋势,但在60和90 Gy同时存在矮化与增高的植株。利用SRAP （sequence related amplified polymorphism）技术对辐照后M1代的黄花蒿植株进行研究,结果表明对照组和处理组之间的差异体现为特异性条带的变化。综合分析表明,12C6+的重离子辐照黄花蒿干种子可以产生显著的当代损伤效应,同时引起植物DNA的多态性变化。     

~(12)C~(6+)离子束与电子束辐照对薰衣草当代诱变效应的比较

采用100 MeV的~(12)C~(6+)离子束和1.2 MeV的电子束辐照薰衣草干种子,研究了2个品种的薰衣草干种子对不同辐射源的辐照生物学效应,以期找到薰衣草干种子的最佳诱变参数。结果表明:不论是薰衣草701还是702,经~(12)C~(6+)离子辐照后,其发芽率表现为先增大后减小的趋势;经电子束辐照后,发芽率随着剂量的升高而降低,电子束辐照后的发芽率要低于~(12)C~(6+)离子束。另外,2个品种的薰衣草经~(12)C~+离子辐照后,其胚轴胚根长度以及幼苗鲜重也较电子束辐照后的大。由此可知,重离子的辐照效果优于电子束的,有利于后期筛选出新的薰衣草突变株。     

~(12)C~(6+)高能重离子辐照大葱损伤及其分子生物学效应

利用30,90,180Gy3种剂量的12C6+重离子束辐照大葱种子,研究其在细胞水平和农艺性状的诱变效应并进行RAPD分析。通过与M1代的研究结果比较后表明:经过不同剂量12C6+重离子照射后能有效地诱导大葱细胞形成微核和染色体畸变,这种诱变效应,在M2代仍然有所表现。M1代大葱结果期的株高、白长、花序直径和种子产量随辐照剂量增加产生明显差别,其中30Gy辐照组增幅最大。大葱总水溶性蛋白质和维生素C的含量在30Gy组中积累最多,在90Gy组有明显下降。与M1代一致,M2代中大葱染色体微核率及RAPD分析所得的DNA多态性比率仍然与辐照剂量呈正相关,但比率整体下降;说明高能量重离子辐照造成的DNA变异在M2代被修复和淘汰。     

姜黄素对重离子辐射损伤小鼠睾丸组织的防护作用研究

探讨姜黄素(Curcumin,简称Cur)对重离子辐射损伤小鼠睾丸组织的防护作用。小鼠灌胃不同剂量的Cur后给予4 Gy剂量~(12)C~(6+)离子束全身单次照射。24 h后对小鼠睾丸组织形态学变化进行观察,并测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性。结果显示:中、高浓度Cur预处理组对小鼠睾丸组织的形态具有较好的保护作用;低、中浓度Cur预处理组MDA含量显著降低(P0.05);与单纯照射组相比,低浓度Cur预处理组SOD活性水平和中浓度Cur预处理组CA了活性水平显著提高(P0.05)。结果表明:Cur对重离子全身辐射小鼠的抗氧化系统有一定的激活效应,对辐射损伤有一定的防护作用,其机制可能与Cur清除自由基,保护脂质和蛋白质有关。     

6MeV/u 12C6+离子对四种作物种子的生物学效应研究

在中科院近代物理研究所兰州重离子研究装置(HIRFL)上,采用46.6MeV/u~(12)C~(6+)离子对四种不同作物种子进行了生物学效应研究,结果表明:重离子的轰击和贯穿,不仅会在种子表面外貌而且也对种子内部细胞造成严重损伤,重离子对种子萌发能力和生长发育均有明显的抑制作用,对生物功能也有影响;在种子的根尖细胞中出现的染色体畸变具有多种类型,而且畸变频率明显高于对照组。重离子对多种细胞器也产生了严重损伤,受损DNA具有一定的修复能力,测定了四种作物种子的半致死剂量及失活截面,找到了四种作物之间辐射敏感性的差异,讨论了细胞器损伤与生理过程异常的关系和受损DNA的修复过程。 The biological effects of 46.6MeV/u ~(12)C~(6+) ions on four kinds of plant seeds were studiedat HIRFL of Institute of Modern Physics (IMP), Academia Sinica. The results indicate that seriousinjuries were presented on not only external features of the seeds but also internal cells due to the bombardment and penetration of the ions. The heavy ions can significantly inhibitgermination and growth of the seeds and can affect physiological functions. In root tip cells ofirradiated seeds...