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12C6+离子对阿维链霉菌累进辐照诱变效应研究
引用本文:王曙阳,陈积红,薄永恒,王丽华,李文建,梁剑平,刘敬,马晓琪.12C6+离子对阿维链霉菌累进辐照诱变效应研究[J].原子核物理评论,2011,28(4):485-488.
作者姓名:王曙阳  陈积红  薄永恒  王丽华  李文建  梁剑平  刘敬  马晓琪
作者单位:中国科学院近代物理研究所, 甘肃 兰州 730000;
摘    要:选用12C6+ 离子束对阿维链霉菌诱变选育高产菌株与原始菌株进行辐照诱变, 研究其累进辐照效应。实验结果表明,在辐照剂量为10 Gy时, 原始菌株比诱变高产菌株存活率高, 抗辐射能力强;辐照剂量高于30 Gy时,诱变高产菌株比原始菌株存活率高, 抗辐射能力强。原始菌株正突变率最高的辐照剂量为50 Gy, 致死率99.43%,正突变率最高, 达34.2%;对诱变高产菌株辐照剂量为30 Gy,致死率94.97%,正突变率最高, 达23.5% 。累进辐照效应降低了最佳辐照剂量。 Mutagenic effect on the mutant high producing strain ZJAV Y1 203 and the original strain ZJAV A1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant high producing Strain at dose of 10 Gy. The mutant high producing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAV Y1 203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.

关 键 词:辐照诱变    累进辐照    效应    存活率    正突变率
收稿时间:1900-01-01

Mutagenic Effect of Streptomyces Avermitilis Progressivity Irradiated by Ion Beam of 12C6+
WANG Shu-yang,CHE Ji-hong,BO Yong-heng,WANG Li-hua,LI Wen-jian,LIANG Jian-ping,LIU Jing,MA Xiao-qi.Mutagenic Effect of Streptomyces Avermitilis Progressivity Irradiated by Ion Beam of 12C6+[J].Nuclear Physics Review,2011,28(4):485-488.
Authors:WANG Shu-yang  CHE Ji-hong  BO Yong-heng  WANG Li-hua  LI Wen-jian  LIANG Jian-ping  LIU Jing  MA Xiao-qi
Institution:Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;Gansu Agriculture University, Lanzhou 730070, China;
Abstract:Mutagenic effect on the mutant high producing strain ZJAV Y1 203 and the original strain ZJAV A1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant high producing Strain at dose of 10 Gy. The mutant high producing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAV Y1 203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.
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