| 雌二醇的水凝胶毛细管电泳免疫分析 |
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| 引用本文： | 苏萍,王永成,张新祥,孙玲,常文保.雌二醇的水凝胶毛细管电泳免疫分析[J].高等学校化学学报,2000,21(6):870-872. |
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| 作者姓名： | 苏萍 王永成 张新祥 孙玲 常文保 |
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| 作者单位： | 北京大学化学与分子工程学院, 北京 100871 |
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| 基金项目： | 国家自然科学基金 !(批准号 :2 9775 0 0 2 ) |
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| 摘 要： | 雌二醇是雌性激素中最重要和作用最强的一种激素 .临床上 ,雌二醇的长期监测可用于研究与激素相关的致癌物作用机理、绝经期妇女的雌激素补充及不孕病人的跟踪治疗等方面 .研究发现 ,人体中雌二醇含量与某些肿瘤如乳腺癌、子宫癌和肝癌等密切相关 1] .由于雌二醇在体液中含量很低 ,难于测定 .免疫分析法的出现给雌二醇的测定带来了变革 ,提高了测定的灵敏度和特异性 .目前测定雌二醇的免疫分析方法有放射免疫分析法 ( RIA) 2 ]、酶免疫分析法 ( EIA) 3～ 7]、荧光免疫分析法 ( FIA) 8]和化学发光与生物发光免疫分析法 9,10 ] .但 R…
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| 关 键 词： | 水凝胶 毛细管电泳 激光诱导荧光 免疫分析 雌二醇 |
| 收稿时间： | 2000-01-06 |
Determination of Estrodiol by Capillary Electrophoretic Immunoassay with Thermally Reversible Hydrogel |
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| SU Ping,WANG Yong-Cheng,ZHANG Xin-Xiang,SUN Ling,CHANG Wen-Bao.Determination of Estrodiol by Capillary Electrophoretic Immunoassay with Thermally Reversible Hydrogel[J].Chemical Research In Chinese Universities,2000,21(6):870-872. |
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| Authors: | SU Ping WANG Yong-Cheng ZHANG Xin-Xiang SUN Ling CHANG Wen-Bao |
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| Institution: | College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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| Abstract: | In this paper, a kind of thermally linear polymer, poly-N-isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. Auseful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pHof buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mLand 0.1-10 ng/mL. |
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| Keywords: | Hydrogel Capillary electrophoresis Laser induced fluorescence Immunoassay Estradiol |
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