A minimal model of self propelled locomotion

Fish locomotion is a complicated problem in the context of fluid–structure interaction and it is still not understood what is linked to biology and what is linked to mechanics. Measurements performed on natural fish and artificial systems reveal that swimming at high Reynolds number is found in a narrow range of Strouhal numbers - a dimensionless combination of the swimming velocity, tail beat amplitude and frequency. With a minimal model of aquatic locomotion, we investigate how this number depends on the numerous parameters at play. We show a strong correlation with the drag coefficient, while the effect of the other parameters can be neglected at the first-order approximation.     

固相萃取-气相色谱-质谱法测定辐照鱼油中2-烷基环丁酮

提出了固相萃取-气相色谱-质谱法测定辐照鱼油中2-烷基环丁酮(2-十二烷基环丁酮和2-十四烷基环丁酮)含量的方法。0.300g辐照鱼油样品用正己烷溶解,ProElut Silica固相萃取柱净化。在气相色谱分离中用HP-5MS色谱柱为固定相,在质谱分析中采用选择离子监测模式。2种2-烷基环丁酮的质量浓度均在0.01~1.0mg·L-1范围内与其峰面积呈线性关系,方法的测定下限(10S/N)为10μg·kg-1。加标回收率在91.2%~96.0%之间,测定值的相对标准偏差(n=6)在3.7%~5.9%之间。     

Synthesis and characterization of CuO nanoparticles utilizing waste fish scale and exploitation of XRD peak profile analysis for approximating the structural parameters

In this research, we strived to utilize waste fish scale (labeo rohita) for synthesizing CuO nano-particles (CuO NPs), which gained much attention due to its distinctive properties and versatile applications. Upon the heat treatment, the collagen content of the fish scale got transformed into gelatin which in turn converted the precursor material into CuO NPs. The X-Ray diffraction (XRD) analysis confirmed the formation of CuO NPs and revealed the structure to be of monoclinic lattice. The structural parameters i.e. crystallite size, lattice parameters, microstrain, dislocation density was evaluated for the synthesized CuO NPs using the XRD data. Scherrer’s Method (SM), Scherrer Equation Average Method (SEAM), Linear Straight Line Method (LSLM), Straight Line Passing the Origin Method (SLPOM), Monshi Scherrer Method (MSM), Williamson-Hall Method (WHM), Size-Strain Plot Method (SSPM), Halder-Wagner Method (HWM) was exploited for the estimation of crystallite size. According to the calculations, the crystallite size was found to be 87 nm, 41 nm, 1980 nm, 62 nm, 66 nm, 28 nm, 13 nm, 13 nm respectively and the dislocation density was found to be 1.32 × 10^-4, 5.95 × 10^-4, 0.002 × 10^-4, 2.60 × 10^-4, 2.29 × 10^-4, 12.75 × 10^-4, 59.17 × 10^-4 and 59.17 × 10^-4 respectively. UV–Vis absorption analysis also confirmed the formation of CuO NPs based on the absorption peak at 262 nm (λ_max) and Tauc Plot method was used to calculate the optical band gap which was 3.84 eV. Functional group, especially the Cu-O bonding was confirmed by the Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) data. Field Emission Scanning Electron Microscopy (FESEM) showed three different shapes of CuO NPs which was also confirmed by Transmission Electron Microscopy (TEM). Particle size was determined based on FESEM and TEM image using imageJ software and also by the Dynamic Light Scattering (DLS) technique. Thermal analysis showed a four stage weight loss in case of Thermogravimetric (TGA) analysis and three conversion steps was observed in Differential Scanning Calorimetric (DSC) analysis. Such synthesis pathway is evidently green and facile for synthesizing CuO NPs with potentiality of various applications and also utilization of waste fish scale is a perquisite.     

Development of fish gelatin-based artificial fish baits incorporating bioattractants from seafood processing waste

The study dealt with evolving an artificial fish bait by incorporating bioattractant concentrates derived from seafood processing wastes by enzymatic hydrolysis namely fish waste concentrate (FWC), squid waste concentrate (SWC), and shrimp processing concentrate (SPWC). They were characterized based on amino acid content and presence of different functional groups using FTIR. Among them, SWC was found to have the highest amino acid content of 60.85mg/100 mg followed by FWC (42.21mg/100 mg) and SPWC (24.82mg/100 mg). The developed artificial fish baits were subjected to protein leaching, solubility in seawater besides testing for acceptability by the red snapper. The study revealed that the bait incorporated with SWC at 3% level was found be the most ideal, with the protein leaching rate of 24.82 mg/g/h, solubility rate of 36.6 mg/g/h and the attractability score of 29/30. The amino acid leaching rate was 3.11 mg/h/100 mg and it was found to contain five amino acids such as glycine, proline, glutamic acid, alanine and serine. The study revealed that the hydrolyzed squid waste concentrate can be incorporated at 3% (w/w) with fish gelatin based biomatrix during gelation to prepare artificial bait.     

Numerical study of a well boat operating at a fish farm in current

Dynamic response of a well boat operating at a fish farm in current is investigated numerically. An objective is to determine the operational conditions of the well boat. In terms of the fish farm, a realistic set-up (with single cage) is considered, including a floating collar, an elastic sinker tube, a flexible-closed net cage and a complex mooring system. A time-domain solution is used to find the steady configuration and response. Transverse viscous current loads are estimated using the cross-flow principle. The drag coefficients are obtained empirically by considering cross-sectional details, free surface and three-dimensional (3D) flow effects. The drag force is experimentally validated. The effect of the ship wake on the net loading is also assessed.The most critical scenario with the well boat placed at the weather side of the fish farm is analyzed in detail. Critical response variables for operational limits are the maximum anchor-line tensions and floater stresses. Numerical results show that the anchor loads will increase more than 40% in small current velocities and up to 90% in high current velocities due to the viscous current loads on the boat. There is also a strong increase of the floating collar deformations and stresses when the well boat is in contact with the floating collar.A sensitivity analysis has been carried out to identify the physical parameters affecting the anchor loads and the maximum stress in the floating collar. From our studies, the anchor loads are more sensitive to current direction, bottom weight system, sinker tube depth and mooring line properties (pretension load, anchor chain weight, etc.) and less sensitive to other parameters such as floating collar stiffness and cross-sectional drag coefficients of the well boat. The shading effect of the well boat on the fish-farm inflow has been examined and appeared not negligible with 4% to 10% reduction of the anchor loads for the studied current conditions. The maximum stress in the floating collar is sensitive to well-boat loads related parameters (current direction, cross-sectional drag coefficient) and pretension load in the anchor line; not so sensitive to net loading related parameters such as sinker tube depth and sinker tube weight.Lastly, the operational conditions of the well boat at the fish farm were discussed. Numerical results show that the maximum stresses in the floating collar should be of major concern. The loads in the mooring lines are moderate compared with the corresponding breaking limits.     

Simultaneous Determination of Selected Veterinary Antibiotics and their Main Metabolites in Fish and Mussel Samples by High-Performance Liquid Chromatography with Diode Array-Fluorescence (HPLC-DAD-FLD) Detection

A reversed-phase high-performance liquid chromatography (RP-HPLC) method for the quantitative determination of 11 antibiotics (drugs) and the main metabolites of five of them in fish tissue and mussel samples were developed, optimized, and validated. The analytes belong to four different classes of antibiotics (sulfonamides, tetracyclines, penicillin, and amphenicols). The analyzed compounds were sulfadiazine and its N⁴-acetylsulfadiazine metabolite; sulfamethazine and its N⁴-acetylsulfamethazine; sulfamerazine and its N⁴-acetylsulfamerazine; sulfamethoxazole; trimetroprim; amoxicillin and its main metabolite amoxicilloic acid; ampicillin and its main metabolite ampicilloic acid; chloramphenicol; thiamphenicol; oxytetracycline; and chlortetracycline. For HPLC analysis, diode array and fluorescence detectors were used. The separation of the analyzed compounds was conducted by means of a C₁₈ (150 mm × 4.6 mm I.D., particle size 5 µm) analytical column with LiChrospher® C₁₈ (4 mm × 4 mm, particle size 5 µm) guard-column. Analyzed drugs were determined within 35 minutes using formic acid 0.1% in water and acetonitrile in gradient elution mode as the mobile phase. The method was applied to the determination of the analytes in tissue of hake (Merluccius merluccius), anchovy (Engraulis encrasicolus), mussel (Mytltus sp.), and wedge sole (Solea solea). The proposed method was also evaluated by a laboratory assay consisting of the determination of the targeted analytes in samples of Cyprinus carpio that were previously administered controlled doses of the antibiotics.     

A Microwave‐assisted Headspace Solid‐phase Microextraction for Rapid Determination of Synthetic Polycyclic and Nitro‐aromatic Musks in Fish Samples

Rapid solvent‐free microwave‐assisted headspace solid‐phase microextraction (MA‐HS‐SPME) coupled with gas chromatography‐mass spectrometry (GC‐MS) was developed to determine synthetic polycyclic and nitro‐aromatic musks in fish samples. Four commonly used synthetic musks, galaxolide (HHCB), tonalide (AHTN), musk xylene (MX) and musk ketone (MK) were employed in the method development and validation. The parameters (microwave irradiation time, irradiation power, amount of water addition, pH value and addition of NaCl) affecting the extraction efficiency of analytes from fish slurry were systematically investigated and optimized. The best extraction conditions were achieved when the fish sample 2‐g mixed with 4‐mL methanol and 15‐mL deionized water (containing 4 g of NaCl, pH 2.0 in a 40‐mL sample‐vial) was microwave irradiated at 80 watt for 5 min. The limits of quantification (LOQ) were 0.4 to 1.2 ng/g in 2‐g of wet tissue. The precision for these analytes, as indicated by relative standard deviations, were less than 9% for both intra‐ and inter‐day analysis. Accuracy, expressed as the mean extraction recovery, was between 80 to 92%. A standard addition method was used to quantitate these four synthetic musks, and the total concentrations ranged from 2.1 to 23.1 ng/g in various fish samples.     

Integrated multidimensional and comprehensive 2D GC analysis of fatty acid methyl esters

Fatty acid methyl ester (FAME) profiling in complex fish oil and milk fat samples was studied using integrated comprehensive 2D GC (GC × GC) and multidimensional GC (MDGC). Using GC × GC, FAME compounds – cis‐ and trans‐isomers, and essential fatty acid isomers – ranging from C18 to C22 in fish oil and C18 in milk fat were clearly displayed in contour plot format according to structural properties and patterns, further identified based on authentic standards. Incompletely resolved regions were subjected to MDGC, with Cn (n = 18, 20) zones transferred to a ²D column. Elution behavior of C18 FAME on various ²D column phases (ionic liquids IL111, IL100, IL76, and modified PEG) was evaluated. Individual isolated Cn zones demonstrated about four‐fold increased peak capacities. The IL100 provided superior separation, good peak shape, and utilization of elution space. For milk fat‐derived FAME, the ²D chromatogram revealed at least three peaks corresponding to C18:1, more than six peaks for cis/trans‐C18:2 isomers, and two peaks for C18:3. More than 17 peaks were obtained for the C20 region of fish oil‐derived FAMEs using MDGC, compared with ten peaks using GC × GC. The MDGC strategy is useful for improved FAME isomer separation and confirmation.