Structural studies on archaeal phytanyl-ether lipids isolated from membranes of extreme halophiles by linear ion-trap multiple-stage tandem mass spectrometry with electrospray ionization

The structures of archaeal glycerophospholipids and glycolipids are unique in that they consist of phytanyl substituents ether linked to the glycerol backbone, imparting stability to the molecules. In this contribution, we described multiple-stage linear ion-trap combined with high resolution mass spectrometry toward structural characterization of this lipid family desorbed as lithiated adduct ions or as the [M−H]⁻ and [M−2H]²⁻ ions by ESI. MSⁿ on various forms of the lithiated adduct ions yielded rich structurally informative ions leading to complete structure identification of this lipid family, including the location of the methyl branches of the phytanyl chain. By contrast, structural information deriving from MSⁿ on the [M−H]⁻ and [M−2H]²⁻ ions is not complete. The fragmentation pathways in an ion-trap, including unusual internal loss of glycerol moiety and internal loss of hexose found for this lipid family were proposed. This mass spectrometric approach provides a simple tool to facilitate confident characterization of this unique lipid family.     

Screening and Identifying of Nephrotoxic Compounds in Lithospermum erythrorhizon Using Live-cell Fluorescence Imaging and Liquid Chromatography Coupled with Tandem Mass Spectrometry

In order to identify the potential nephrotoxic compounds in traditional Chinese medicine Lithospermum erythrorhizon,it was separated into serial fractions according to their polarities.An in vitro method was utilized to determine the nephrotoxicity of these fractions with the help of fluorescence image analysis.As a result,the primary fraction A05 and its secondary fractions C06 C09 and C12 C14 were found to have significant toxicity to LLC-PK1 cell line,as determined by the survive rate less than 20% after...     

Implementing remote controlled-NOT gates and entanglement swapping via geometric phase gates in ion-trap systems

We propose a scheme for the implementation of remote controlled-NOT gates and entanglement swapping via geometric phase gates in ion-trap systems. The proposed scheme uses the two ground states of the $\Lambda$-type ions as memory instead of the vibrational mode. And the system is robust against the spontaneous radiation and the dephasing.     

EASC荧光标记和LC-APCI-MS检测环境水样中游离脂肪胺

采用新型荧光标记试剂10-乙基吖啶酮-2-磺酰氯(EASC)作为柱前衍生试剂, 在Hypersil BDS C18反相色谱柱上(4.6 mm×200 mm, 10 μm i.d.), 采用梯度洗脱在20 min内实现了12种EASC-脂肪胺衍生物的快速基线分离. 最佳检测波长为λex/λem=270 nm/430 nm. EASC与常用的Dansyl-Cl相比具有更强的光致发光特性(紫外和荧光): 紫外吸收强度之比为3.2∶1, 相对荧光强度之比为30.0∶1~105.4∶1, 荧光量子效率之比为43.0∶1. 通过荧光检测及离子阱大气压化学电离源的正离子模式获得了胺类组分的准确定量和相应的柱后质谱鉴定. 质谱灵敏度之比为1.6∶1~6.2∶1. 建立的方法对环境水样中脂肪胺类化合物的测定具有快速、准确和重现性良好等优点, 回归系数大于0.9995, 检出限为4.0~12.7 fmol.     

Study of the Fragmentation of D-Glucose and Alkylmonoglycosides in the Presence of Sodium Ions in an Ion-Trap Mass Spectrometer

Abstract

Using electrospray ion-trap mass spectrometry, the fragmentation of D-glucose and alkylmonoglycopyranosides (alkyl-GPs) was studied. In the presence of Na⁺, B₁ and ^0,2A fragmentations were observed. The alkyl-GPs also showed a ^2,5Afragmentation. A cluster containing no carbon atoms and adducts of this cluster with neutral molecules were observed. Standards of alkylmonoglycofuranosides (alkyl-GFs) were not available; however, their fragmentation was studied by high-performace liquid chromatography–mass spectrometry (HPLC-MS) and HPLC-MS² using an industrial mixture of alkylpolyglycosides. The cluster and its adducts were more easily formed by the alkyl-GPs than by the alkyl-GFs, but the ^0,2A cross-ring cleavage was more easily produced by the alkyl-GFs.     

Two Feasible Schemes for Preparing Cluster States with Ion-Trap Setup

We present two schemes for preparing cluster states with atomic qubits in an ion-trap system. In the first scheme an auxiliary atomic level is needed. While in the second scheme an additional classical driven field is used, and the multi-ion cluster states can be generated by one step. Both the schemes are insensitive to thermal motion of the ions, all the facilities used in them are well within state of the art.     

Analysis of perfluorinated compounds in biota by microextraction with tetrahydrofuran and liquid chromatography/ion isolation-based ion-trap mass spectrometry

An analytical method combining both a simple, fast and efficient solvent microextraction and a sensitive and selective monitoring mode, based on ion isolation ion-trap mass spectrometry (MS), was developed for analysis of perfluorinated compounds (PFCs) in biota. The method involved the vortex-shaking of 0.2 g of tissue sample and 800 μL of tetrahydrofuran (THF):water (75:25, v/v) for 7 min, subsequent centrifugation for 13 min and direct quantitation of PFCs in the extract against solvent-based calibration curves. Selection of solvent composition was based on Hildebrand solubility parameters and their components (i.e. dispersion, dipole–dipole and hydrogen bonding forces). Recoveries in samples for PFCs with hydrocarbon chain lengths between C₄ and C₁₄ ranged from 85 to 111%, with relative standard deviations between 1 and 11%. The ion isolation monitoring mode, proposed for the first time for ion-trap-MS quantitation, proved to be effective in avoiding space-charge effects caused by co-eluting matrix components while keeping the sensitivity of full scan MS operation. Detection limits of the method were in the range 0.8−6 ng g⁻¹ for perfluoroalkyl carboxylates (PFACs) and 0.4–0.8 ng g⁻¹ for perfluoroalkyl sulfonates (PFASs) in wet weight samples. The method was validated using a reference material made up of flounder muscle and by comparison with triple quadrupole MS measurements and it was applied to the determination of PFCs in liver and muscle samples from sea birds and fishes. Only PFASs were found in samples at quantifiable levels (2.9 and 13.1 ng g⁻¹) while PFACs were below the respective quantitation limits. This method allows quick and simple microextraction of PFCs with minimal solvent consumption, while delivering accurate and precise data.     

Determination of the Alternaria mycotoxin tenuazonic acid in cereals by high-performance liquid chromatography–electrospray ionization ion-trap multistage mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine

Tenuazonic acid (TA) is a major Alternaria mycotoxin. In the present work a novel approach for the detection of TA in cereals by liquid chromatography–ion-trap multistage mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine is described. The product of the derivatization reaction and its major MS² fragments were characterised by Fourier transform-ion cyclotron resonance tandem mass spectrometry. Without preconcentration, the established method features a limit of detection of 10 μg/kg using 2 g of sample in a rapid workup procedure. Accuracy, precision and linearity were evaluated in the working range of 50–5000 μg/kg. TA was detected in 13 and quantified in 3 out of 27 cereal samples obtained from a local supermarket, the average content being 49 μg/kg (highest incidence: 851 ± 41 μg/kg).     

利用离子阱技术实现1→2和1→3的最优非对称经济型相位协变克隆的方案

我们提出利用离子阱技术来实现1→2和1→3的最优非对称经济型相位协变克隆的实验可行性方案.在这两个方案中集体振动模开始处于真空态.在第一个方案中,我们先用激光驱动第一个离子,然后把振动模的态映射到第二个离子上.在第二个方案中,先用两束不同的激光驱动两个离子,然后把振动模的态映射到第三个离子上.这两个方案都是以共振边带激发为基础的,因此用现在的离子阱技术是可以实现的.     

在离子阱体系中通过Bell态来产生簇态

我们对已存在的在离子阱体系中产生簇态的方案进行修改.本文中提出的方案是基于Bell态和控制相位门(cz门)的实现.通过Bell态和控制相位门,我们就可以产生4比特(qubit)以及6比特的簇态.