Formation of Supramolecular Nanostructures through in Situ Self-Assembly and Post-Assembly Modification of a Biocatalytically Constructed Dipeptide Hydrazide**

Aqueous self-assembly of short peptides has attracted growing attention for the construction of supramolecular materials for various bioapplications. Herein, we describe how the thermolysin-assisted biocatalytic construction of a dipeptide hydrazide from an N-protected amino acid and an amino acid hydrazide leads to the formation of thermally stable supramolecular hydrogels. In addition, we demonstrate the post-assembly modification of the supramolecular architectures constructed in situ tethering hydrazide groups as a chemical handle by means of fluorescence imaging.     

Global stability of a multistrain SIS model with superinfection and patch structure

We study the global stability of a multistrain SIS model with superinfection and patch structure. We establish an iterative procedure to obtain a sequence of threshold parameters. By a repeated application of a result by Takeuchi et al. [Nonlinear Anal Real World Appl. 2006;7:235–247], we show that these parameters completely determine the global dynamics of the system: for any number of patches and strains with different infectivities, any subset of the strains can stably coexist depending on the particular choice of the parameters.     

Glycyl-alanyl-histidine protects PC12 cells against hydrogen peroxide toxicity

Background

Peptides with cytoprotective functions, including antioxidants and anti-infectives, could be useful therapeutics. Carnosine, β-alanine-histidine, is a dipeptide with anti-oxidant properties. Tripeptides of Ala-His-Lys, Pro-His-His, or Tyr-His-Tyr are also of interest in this respect.

Results

We synthesized several histidine-containing peptides including glycine or alanine, and tested their cytoprotective effects on hydrogen peroxide toxicity for PC12 cells. Of all these peptides (Gly-His-His, Ala-His-His, Ala-His-Ala, Ala-Ala-His, Ala-Gly-His, Gly-Ala-His (GAH), Ala-His-Gly, His-Ala-Gly, His-His-His, Gly-His-Ala, and Gly-Gly-His), GAH was found to have the strongest cytoprotective activity. GAH decreased lactate dehydrogenase (LDH) leakage, apoptosis, morphological changes, and nuclear membrane permeability changes against hydrogen peroxide toxicity in PC12 cells. The cytoprotective activity of GAH was superior to that of carnosine against hydrogen peroxide toxicity in PC12 cells. GAH also protected PC12 cells against damage caused by actinomycin D and staurosporine. Additionally, it was found that GAH also protected SH-SY5Y and Jurkat cells from damage caused by hydrogen peroxide, as assessed by LDH leakage.

Conclusion

Thus, a novel tripeptide, GAH, has been identified as having broad cytoprotective effects against hydrogen peroxide-induced cell damage.

     

All-Solid-State Rechargeable Air Batteries Using Dihydroxybenzoquinone and Its Polymer as the Negative Electrode

A proof-of-concept study was conducted on an all-solid-state rechargeable air battery (SSAB) using redox-active 2,5-dihydroxy-1,4-benzoquinone (DHBQ) and its polymer (PDBM) and a proton-conductive polymer (Nafion). DHBQ functioned well in the redox reaction with the solid Nafion ionomer at 0.47 and 0.57 V vs. RHE, similar to that in acid aqueous solution. The resulting air battery exhibited an open circuit voltage of 0.80 V and a discharge capacity of 29.7 mAh g_DHBQ⁻¹ at a constant current density (1 mA cm⁻²). With PDBM, the discharge capacity was much higher, 176.1 mAh g_PDBM⁻¹, because of the improved utilization of the redox-active moieties. In the rate characteristics of the SSAB-PDBM, the coulombic efficiency was 84 % at 4 C, which decreased to 66 % at 101 C. In a charge/discharge cycle test, the capacity remaining after 30 cycles was 44 %, which was able to be significantly improved, to 78 %, by tuning the Nafion composition in the negative electrode.     

Catalytic Activity of Molybdenum Complexes Bearing PNP-Type Pincer Ligand toward Ammonia Formation

We newly designed and prepared a novel molybdenum complex bearing a 4-[3,5-bis(trifluoromethyl)phenyl]pyridine-based PNP-type pincer ligand, based on the bond dissociation free energies (BDFEs) of the N−H bonds in molybdenum-imide complexes bearing various substituted pyridine-based PNP-type pincer ligands. The complex worked as an excellent catalyst toward ammonia formation from the reaction of an atmospheric pressure of dinitrogen with samarium diiodide as a reductant and water as a proton source under ambient reaction conditions, where up to 3580 equivalents of ammonia were formed based on the molybdenum atom of the catalyst. The catalytic activity was significantly improved by one order of magnitude larger than that observed when using the complex before modification.     

A finite element analysis of strain localization for soft rock using a constitutive equation with strain softening

Summary It is well known that deformation and/or strain of geological materials localize when they come close to the failure state. In the present: study, we try to analyze the deformation problem using a constitutive relation with strain hardening and strain softening. The constitutive model of a soft rock and overconsolidated clay using an elasto-plastic constitutive theory with memory was originally developed by Adachi and Oka. This type of formulation is shown to be easily applied to analyze the material behavior of strain softening because there is a similarity to that in viscoplasticity. Using the proposed model, the loss of uniqueness of the solution to the initial value problem can be avoided and a special or complicated numerical technique, e.g., an arc length method, does not need to be used. When we use constitutive equations with strain softening in a finite element analysis, there is a problem of strong mesh size dependency of numerical results. To remedy the mesh size dependency, we generalize the Adachi-Oka model based on the concept of non-localization by Bazant. We apply the proposed constitutive model to the behavior of a sedimentary soft rock in the drained triaxial compression test. It is found that mesh size dependency becomes smaller using the non-localization of the constitutive model.

---

Eine Finite-Element-Analyse der lokalisierten Verformung von weichem Gestein bei einem Stoffgesetz mit Entfestigung

Übersicht Bekanntlich findet bei geologischen Stoffen in der Nähe des Versagenszustandes eine Lokalisierung der Verformung statt. Die Analyse dieses Problems wird in diesem Beitrag auf der Grundlage eines Stoffansatzes mit Ver- und Entfestigung unternommen. Entwickelt wurde das zugrunde gelegte elastischplastische Stoffmodell mit Gedächtnis von Adachi und Oka für weiches Gestein und übermäßig verdichteten Ton. Wegen der Ähnlichkeit zur Viskoplastizität läßt sich diese Formulierung des Stoffgesetzes leicht auf die Analyse des Verhaltens von entfestigendem Material anwenden, da der Eindeutigkeitsverlust der Lösung des Anfangswertproblems vermieden wird und besondere Rechenverfahren wie etwa die Bogenlängenmethode nicht benötigt werden. Bei der Benutzung von Stoffgesetzen in einer Finite-Element-Rechnung hängen die Ergebnisse stark von der Netzeinteilung ab. Um dies abzustellen, wird das Modell von Adachi und Oka auf der Grundlage von Bazant's Konzept der Nichtlokalisierung verallgemeinert. Anwendungsbeispiel ist das Verhalten von weichem Gestein im drainierten Triaxial-Test. Es zeigt sich, daß mit dem Konzept der Nichtlokalisierung im Stoffmodell der Einfluß der Netzeinteilung geringer wird.

---

---

Presented at the workshop on Numerical Methods for Localization and Bifurcation of Granular bodies, held at the Technical University of Gdask (Poland), September 25–30, 1989.     

On global stability of an HIV pathogenesis model with cure rate

In this paper, applying both Lyapunov function techniques and monotone iterative techniques, we establish new sufficient conditions under which the infected equilibrium of an HIV pathogenesis model with cure rate is globally asymptotically stable. By giving an explicit expression for eventual lower bound of the concentration of susceptible CD4⁺ T cells, we establish an affirmative partial answer to the numerical simulations investigated in the recent paper [Liu, Wang, Hu and Ma, Global stability of an HIV pathogenesis model with cure rate, Nonlinear Analysis RWA (2011) 12 : 2947–2961]. Our monotone iterative techniques are applicable for the small and large growth rate in logistic functions for the proliferation rate of healthy and infected CD4⁺ T cells. Copyright © 2014 John Wiley & Sons, Ltd.