Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide, and between the hybridization of 5' CTT CAG GTC ATG AGC CTG AT 3' and its complementary 3' GAA GTC CAG TAC TCG GAC TA 5' oligonucleotide, by the oblique-incidence reflectivity difference (OI-RD) method. The dynamic curves of OI-RD signals, corresponding to the kinetic processes of biomolecular combination or hybridization, are acquired. In our case, the combination of epoxy groups with rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide need almost one and a half hours and about two hundred seconds, respectively; and the hybridization of the two oligonucleotides needs about five hundred seconds. The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions.     

Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method

We label-free detected the biological process of preparing a microarray that includes 400 spots of mouse immunoglobulin G （IgG） as well as the specific hybridization between mouse IgG and goat anti-mouse IgG by an oblique-incidence reflectivity difference （OI-RD） method. The detection results after each process including printing, washing, blocking, and hybridization, demonstrate that the OI-RD method can trace the preparation process of a microarray and detect the specific hybridization between antigens and antibodies. OI-RD is a promising method for label-free and high-throughput detection of biological microarrays.     

钙钛矿氧化物异质结的光电特性研究进展

光与物质相互作用可以产生各种光学现象,其中光电效应是非常重要的现象之一.文中集中回顾了文章作者在钙钛矿氧化物异质结的光电效应研究中的进展.在钙钛矿氧化物异质结中,分别观测到了传统的纵向光电效应和反常的横向光电效应,并通过对含时的漂移-扩散方程的自洽求解,从理论上分别揭示了钙钛矿氧化物异质结纵向和横向光电效应的动态过程.文章首先介绍了钙钛矿氧化物异质结纵向光电效应的研究进展,接着概述了钙钛矿氧化物异质结横向光电效应研究的进展.最后对氧化物异质结的纵向和横向光电效应的潜在应用前景进行展望.     

Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference （OI-RD） analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.     

钙钛矿氧化物异质结自旋极化输运机制研究

文章介绍了一个基于弱Hund耦合规则以及载流子漂移扩散机制所提出的关于钙钛矿氧化物p-n异质结构的自旋极化输运机制的物理模型．该理论不仅可以很好地解释由具有负磁阻效应的La0.9Sr0．1MnO3（LSMO）与非磁性的SrNb0.01Ti0.99O3（SNTO）所组成的异质结中所存在的正磁电阻效应，同时揭示了该体系中LSMO在界面区域的载流子与远离界面区域的载流子具有不同的自旋极化方向．这一结果将为理解钙钛矿氧化物异质结及多层膜的自旋极化输运机制开辟了一条新的途径．