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Nonlinear Dynamics - The modal truncation is usually used in the dynamic response analysis of flexible multibody systems, in which, appropriate selection of modal shapes is very critical to achieve... 相似文献
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Changliang Zhu Jingliang Zhang Liyan Li Jing Zhang Yanhui Jiang Zhaopeng Shen Huashi Guan Xiaolu Jiang 《Applied biochemistry and biotechnology》2017,182(1):216-228
A hyaluronate lyase was obtained by cultivating Arthrobacter globiformis strain A152. The enzyme was purified to homogeneity from the supernatant by ammonium sulfate fractionation, Q Sepharose Fast Flow, and Sephadex G-100 chromatography. The purification resulted in a 32.78-fold increase in hyaluronate lyase activity with specific activity of 297.2 U/mg. The molecular weight of the enzyme determined by SDS-PAGE was approximately 73.7 kDa. Using hyaluronic acid (HA) as a substrate, the maximal reaction rate (Vmax) and the Michaelis–Menten constant (Km) of hyaluronate lyase were found to be 4.76 μmol/min/ml and 0.11 mg/ml, respectively. The optimum pH and temperature values for hyaluronate lyase activity were pH 6.0 and 42 °C, respectively. This enzyme was stable at pH 4–10, 5–7, and 5–7 at 4, 37, and 42 °C, respectively. Investigation about temperature effects on hyaluronate lyase displayed that it was stable at 30–37 °C and also showed high activity at 37 °C. The enzymatic activity was enhanced by Ca2+ and was strongly inhibited by Cu2+ and SDS. These properties suggested that the hyaluronate lyase in this study could bring promising prospects in medical and industry applications. 相似文献
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酶作用机制的模糊以及影响异相体系因素的大量存在, 使得纤维素水解的酶催化过程高度复杂, 很难为之建立理论模型. 采用非理论模型人工神经网络模拟和预测了纤维素酶水解反应, 并与常用的响应面模型进行了比较. 选取加酶量 X1, 底物浓度 X2 和反应时间 X3 作为自变量, 还原糖浓度 Y1 和原料转化率 Y2 作为响应值. 结果表明, 人工神经网络模型比响应面模型更适合作为研究纤维素酶水解的动力学工具. 在模拟过程中, 除中心试验点外, 只有 1 个试验点上人工神经网络模拟值 Y2 产生的误差大于响应面模型. 在预测过程中, 人工神经网络模型的预测值都比响应面模型更接近实验值. 相似文献
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Shande Liu Lulu Dong Baitao Zhang Jingliang He Zhaowei Wang Jian Ning Ruihua Wang Xunmin Liu 《中国光学快报(英文版)》2014,12(3):31402-40
We demonstrate a high power continuous-wave (CW) and acoustic-optically (AO) Q-switched 1314-nm laser with a diode-side-pumped Nd:YLF module. A maximum CW output power of 21.6 W is obtained with a diode pump power of 180 W, corresponding to an optical-to-optical conversion efficiency of 12.0% and a slope efficiency of 16.1%. In the Q-switching operation, a highest pulse energy of 3.8 mJ is obtained at a pulse repetition rate of 1 kHz. The shortest pulse width and maximum single peak power are 101.9 ns and 37.3 kW, respectively. 相似文献
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[structure: see text]. The oxidation of selected anions (N3-, SCN-, I-, and Br-) by ceric ammonium nitrate (CAN) in the presence of substituted cyclopropyl alcohols provides a novel approach to beta-functionalized ketones. The protocol has a number of advantages including short reaction times, ease of reagent handling, and mild, neutral reaction conditions. Overall, this method provides an alternative pathway to important starting materials and intermediates in organic synthesis. 相似文献
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A stochastic prey-predator model with functional response is investigated in this paper. A complete threshold analysis of coexistence and extinction is obtained. Moreover, we point out that the stochastic predator-prey model undergoes a stochastic Hopf bifurcation from the viewpoint of numerical simulations. Some numerical simulations are carried out to support our results. 相似文献