Modeling of the glycine tripeptide cyclization in the Ser65Gly/Tyr66Gly mutant of green fluorescent protein

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A Novel Streptavidin–luciferase Fusion Protein: Preparation,Properties and Application in Hybridization Analysis of DNA

A streptavidin–luciferase fusion protein comprising the thermostable mutant form of firefly luciferase Luciola mingrelica and minimal core streptavidin was constructed. The streptavidin–luciferase fusion was mainly produced in a tetrameric form with high luciferase and biotin‐binding activities. It was shown that fusion has the same K_m values for ATP and luciferin and the bioluminescence spectra as initial luciferase. The linear dependence of the bioluminescence signal on the content of the fusion was observed within the range of 10^?18–10^?13 mol per well. Successful application of obtained fusion in a biospecific bioluminescence assay based on biotin–streptavidin interactions was demonstrated by the example of a specific DNA hybridization analysis. A DNA hybridization analysis for Escherichia coli cells identification was developed using unique for these cells gadB fragment encoding glutamate decarboxylase. The amplified biotinylated GadB fragments were hybridized with the immobilized oligonucleotide probes; then, the biotin in the DNA duplexes was detected using the streptavidin–luciferase fusion protein. To reach the high sensitivity of the assay, we optimized the conditions of the assay. It was shown that the use of Pluronic for plate modification resulted in a significant reduction in the DNA detection limit which finally was 0.4 ng per well.     

Studying the Effect of the Spatial Frequency and Amplitude of Corrugation on the Stress–Strain State of Cylindrical Shells

An approach developed earlier to solve two-dimensional static problems for noncircular cylindrical shells is used to analyze the effect of the spatial frequency and amplitude of corrugation on the stress–strain state of shells. The results of the analysis are presented in the form of plots and tables     

Solving the Stress Problem for a Finite Cylinder by the Schwarz Method with Hybrid Approximations

An axisymmetric elastic problem is solved by a heterogeneous numerical scheme of the alternating Schwarz method based on the finite- and boundary-element methods. The Schwarz method and the modified scheme are compared. A relaxation parameter is used to accelerate the iterative process     

Free vibrations of rectangular orthotropic shallow shells with varying thickness

The paper proposes a numerical-analytic approach to studying the free vibrations of orthotropic shallow shells with double curvature and rectangular planform. The approach is based on the spline-approximation of unknown functions. Calculations are carried out for different types of boundary conditions. The influence of the mid-surface curvature and variable thickness on the behavior of dynamic characteristics is studied __________ Translated from Prikladnaya Mekhanika, Vol. 43, No. 6, pp. 102–115, June 2007.     

On One Approach to the Solution of Stress Problems for Noncircular Hollow Cylinders

An approach is proposed to solve three-dimensional stress problems for noncircular hollow cylinders. The end conditions are such that the problem can be reduced to a two-dimensional problem. This problem is reduced to a one-dimensional problem by introducing additional functions into the resolvable system of equations. These functions are determined using discrete Fourier series. The one-dimensional problem is solved by a stable numerical method. As an example, the stress state of cylinders with an elliptic cross section is analyzed depending on their thickness and degree of ellipticity.