测定麦芽糖转糖苷反应体系组成的高效液相色谱法

建立了分析低聚异麦芽糖组成的高效液相色谱法，采用Spherisorb-NH2色谱柱，示差折光检测器，乙腈－水（体积比70：30）为流动相，外标法定量测定；结果显示各糖质量浓度在0．1－10g/L范围内与峰面积呈良好线性关系，相关系数为0．999 0－0．999 7；应用法跟踪了pH5．0的柠檬酸－柠檬酸钠缓冲溶液中以α－葡萄糖转苷酶为催化剂在58℃温度下的麦芽糖转糖苷反应，分析了反应体系组成随时间的变化，得到了上述反应条件下麦芽糖最大限度地转化为低聚异麦芽糖的最佳反应时间为24h；该法快捷、简单、准确，可用于低聚异麦芽糖生产的质量控制。     

用线性插值法求单摆运动周期的近似解

利用线性插值的方法,提出了一个任意摆角条件下的单摆运动周期的近似公式.并利用Mathematica软件将该公式与精确解进行了比较,还与其他近似公式进行了对比.结果表明,该公式的近似程度相当好.     

Solution Transformations and Their Effects on the Systems with Open Boundary Conditions

The solution transformations and properties of the R-matrices for two-component systems under these transformations are analyzed in details.Not all transformed R-matrices can be put into the Skalyanin‘s formalism.For those R-matrices with all required properties,the effects of solution transformations to the six-and eight-vertex systems with open boundary conditions are discussed.these effects can be one of the following types:the Hamiltonian is invariant or transposition-invariant or made in a similarity transformation,or its coupling coefficients are multiplied by an overall factor,or the spin of the system is rotated around the z axis or/and reflected with respect to some plane.In these cases,the transformed systems remain to be integrable.     

Preparation of Polyclonal Antibodies Against Testis-specific Protease 50 and Characterization of Antibody Specificity

Introduction TheTSP50genehasbeenidentifiedasoneofthe testis specificoncogenes,whichisexpressedathigh levelsinapproximately92%ofhumanbreastcancer samples,makingitanattractivemolecularmarkerand apotentialtargetfordiagnosisandtherapy[1].Itisho mologoustomany…     

Application of symplectic algorithms to QCT calculation:H+H_2 system

The basic theory of symplectic algorithm was introduced. A comparison between Runge-Kutta method and symplectic integration method was preformed in the simulation of the long time behavior of H + H2 system on BKMP potential energy surface. Our results reveal a dis-sipative behavior in the integral of ordinary differential equation by the fourth order Runge-Kutta method, which causes incorrect simulation results in QCT calculations. However, when the symplectic integration method is applied, the dissipative behavior is not found in the same system. When the initial state is the same, the energy deviation of fourth order symplectic integral method is almost one percent of that of fourth order Runge-Kutta method in a 60000-step simulation, and that of sixth order symplectic integral method is much less. These results show that the symplectic integral methods are always the better choice in the integral calculation of the long time behavior in maintaining energy conservation.     

辅酶Ⅰ在β－萘醌磺酸根修饰金带电极上的电化学还原

研制了聚毗咯／ＮＱＳ修饰金带电极，讨论了ＮＱＳ／ＰＰｙ的电聚合过程及实验条件对金电极上吡咯聚合物膜性质的影响；用成核及生长理论解释了阴离子ＮＱＳ对电聚合过程计时电流曲线的影响，优化了ＮＱＳ／ＰＰｙ／Ａｕ电极的制备条件．该修饰电极稳定性好，对辅酶Ⅰ在金电极上的还原有催化作用．在ｐＨ＝７．０时催化电流在４．０×１０￣（－５）～１．５×１０￣（－３）ｍｏｌ／Ｌ范围内与ＮＡＤ￣＋　浓度有线性关系，探讨了电催化作用的机制．     

变分过渡态理论对OCS,CO2,CS2体系的研究

本文用变分过渡态理论,首次对无鞍点的释能反应O+CS→CO+S、O+CO→CO+O和S+CS→CS+S进行了动力学研究,考察了反应动力学瓶颈区性质,计算了反应的速度常数,并将O+CS反应的速度常数值与实验值及经典轨迹计算结果进行了比较,三者基本相符.     

偶氮胂-DBS用于光度法测定牛血清白蛋白

在室温下,于pH 2.5的Britton-Robinson缓冲溶液中及非离子表面活性剂OP存在下,牛血清白蛋白(BSA)与偶氮胂-DBS的复合物的最大吸收在514 nm波长处,但显色复合物与试剂空白吸光度之差在370 nm处最大,选择在370 nm处测定,反应迅速完成。非离子表面活性剂OP可提高反应灵敏度及复合物稳定性。乙醇可提高体系灵敏度。BSA的质量范围在0~60.0 mg.L-1范围内符合比耳定律,方法表观摩尔吸光率3ε70 nm=8.58×105L.mol-1.cm-1。方法用于测定血清样品中蛋白质的总量,5次测定值的RSD为0.60%,回收率为98.0%。     

载铜5A分子筛在汽油模拟体系中脱硫性能的研究

近年,美国环保局计划将汽油中硫的质量分数从当前的300×10-6降到2006年的30×10-6,欧盟也已经通过了新的汽油硫质量分数标准为30×10-6~50×10-6,德国甚至提出计划使用无硫汽油[1]。中国汽油硫的质量分数高达800×10-6以上,与世界汽油品质距离甚远。因此汽油中硫化物的脱除成为当务之急。目前,脱硫技术主要有催化裂化脱硫、催化加氢脱硫、水蒸气脱硫、生物催化脱硫、吸附精制脱硫、氧化脱硫等[2~5]。吸附精制法具有净化度高、能耗低、易于操作等优点,高效的脱硫吸附剂制备是过程开发的关键。负载金属离子的活性纤维是脱除汽油中硫醇的一种…     

玉米素和激动素的伏安行为

玉米素和激动素在０．１ｍｏｌ／Ｌ的醋酸－醋酸钠底液里能还原，产生很好的极谱峰，检测灵敏度可达２．５×１０＾－＾８ｍｏｌ／Ｌ，峰电位分别为－１．１８Ｖ和－１．１７Ｖ（对Ａｇ／ＡｇＣｌ）。测定了电极反应决定步骤的αｎα和电化学反应的标准速率常数ｋｓ，研究了它们对Ｃｕ＾２＾＋，Ｅｕ＾３＾＋，Ｃｄ＾２＾＋，Ｚｎ＾２＾＋的电极不产生的影响。