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Poyye Dsouza Priya Swetha Jospeh Sonia Kannan Sapna K. Sudhakara Prasad 《Current Opinion in Electrochemistry》2021
Even though global health has been steadily improved, the global disease burden associated with communicable and non-communicable diseases extensively increased healthcare expenditure. The present COVID-19 pandemic scenario has again ascertained the importance of clinical diagnostics as a basis to make life-saving decisions. In this context, there is a need for developing next-generation integrated smart real-time responsive biosensors with high selectivity and sensitivity. The emergence of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas biosensing systems has shown remarkable potential for developing next-generation biosensors. CRISPR/Cas integrated electrochemical biosensors (E-CRISPR) stands out with excellent properties. In this opinionated review, we illustrate the rapidly evolving applications for E-CRISPR-integrated detection systems towards biosensing and the future scope associated with E-CRISPR based diagnostics. 相似文献
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P. P. Manzur Ali K. Sapna K. R. Rekha Mol Sarita G Bhat M. Chandrasekaran K. K Elyas 《Applied biochemistry and biotechnology》2014,173(1):167-178
Protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and food preservative applications. Fungi have been recognized as sources of protease inhibitors, although there are only few such reports on mushrooms. This work reports the purification and characterization of a trypsin inhibitor from the fruiting body of edible mushroom Pleurotus floridanus (PfTI) and its effect on the activity of microbial proteases. The protease inhibitor was purified up to 35-fold by DEAE-Sepharose ion exchange column, trypsin-Sepharose column and Sephadex G100 column. The isoelectric point of the inhibitor was 4.4, and its molecular mass was calculated as 37 kDa by SDS-PAGE and 38.3 kDa by MALDI-TOF. Inhibitory activity confirmation was by dot-blot analysis and zymographic activity staining. The specificity of the inhibitor toward trypsin was with Ki of 1.043?×?10?10 M. The inhibitor was thermostable up to 90 °C with maximal stability at 30 °C, active over a pH range of 4–10 against proteases from Aspergillus oryzae, Bacillus licheniformis, Bacillus sp. and Bacillus amyloliquefaciens. Results indicate the possibility of utilization of protease inhibitor from P. floridanus against serine proteases. 相似文献
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Trends in microRNA detection 总被引:1,自引:0,他引:1
MicroRNAs (miRNAs) are short, ~22 nucleotide length RNAs that perform gene regulation. Recently, miRNA has been shown to be
linked with the onset of cancer and other diseases based on miRNA expression levels. It is important, therefore, to understand
miRNA function as it pertains to disease onset; however, in order to fully understand miRNA’s role in a disease, it is necessary
to detect the expression levels of these small molecules. The most widely used miRNA detection method is Northern blotting,
which is considered as the standard of miRNA detection methods. This method, however, is time-consuming and has low sensitivity.
This has led to an increase in the amount of detection methods available. These detection methods are either solid phase,
occurring on a solid support, or solution phase, occurring in solution. While the solid-phase methods are adaptable to high-throughput
screening and possess higher sensitivity than Northern blotting, they lack the ability for in vivo use and are often time-consuming.
The solution-phase methods are advantageous in that they can be performed in vivo, are very sensitive, and are rapid; however,
they cannot be applied in high-throughput settings. Although there are multiple detection methods available, including microarray
technology, luminescence-based assays, electrochemical assays, etc., there is still much work to be done regarding miRNA detection.
The current gaps of miRNA detection include the ability to perform multiplex, sensitive detection of miRNA with single-nucleotide
specificity along with the standardization of these new methods. Current miRNA detection methods, gaps in these methods, miRNA
therapeutic options, and the future outlook of miRNA detection are presented here. 相似文献
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Kumar Sunil Jeon H. C. Kang T. W. Kalia Rajesh Sharma J. K. Panwar Sanjay Kalia Sapna Sharma Vandana Choubey R. K. 《Russian Journal of Physical Chemistry A, Focus on Chemistry》2017,91(13):2666-2670
Russian Journal of Physical Chemistry A - In the present work, the effect of temperature and moisture has been studied on nano-porous polycarbonate membranes of size 15, 50, and 80 nm, respectively... 相似文献
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The optimal current difference lattice hydrodynamic model is extended to investigate the traffic flow dynamics on a unidirectional single lane gradient highway. The effect of slope on uphill/downhill highway is examined through linear stability analysis and shown that the slope significantly affects the stability region on the phase diagram.Using nonlinear stability analysis, the Burgers, Korteweg-deVries(KdV) and modified Korteweg-deVries(mKdV) equations are derived in stable, metastable and unstable region, respectively. The effect of reaction coefficient is examined and concluded that it plays an important role in suppressing the traffic jams on a gradient highway. The theoretical findings have been verified through numerical simulation which confirm that the slope on a gradient highway significantly influence the traffic dynamics and traffic jam can be suppressed efficiently by considering the optimal current difference effect in the new lattice model. 相似文献
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Investigations on the phase diagrams of two bi-component mixtures of liquid crystals are presented. Whereas in one of the bi-component systems, smectic A (SmA) phase is seen to be induced, in the other case the so-called nematic “island” has been observed. The dielectric properties of magnetically oriented sample forming nematic phase and induced smectic phase in one of the mixtures, and a nematic “island” and SmA phase in the other mixture, were measured in the frequency range from 1?Hz to 10?MHz at different temperatures. The transition temperatures obtained from the polarizing microscope and differential scanning calorimeter measurements agree well with the dielectric relaxation data. 相似文献
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