The ArNe-N2O van der Waals trimer: a high resolution spectroscopic study of its rotational spectrum,structure and dynamics

A new ternary van der Waals complex of the type rare gas-rare gas'-linear molecule, ArNe-N₂O, was investigated using a pulsed molecular beam cavity Fourier transform microwave spectrometer. The rotational spectra of six isotopomers of the trimer were studied in detail. These include Ar²⁰Ne-¹⁴N¹⁴NO, Ar²²Ne-¹⁴N¹⁴NO, Ar²⁰Ne-15N¹⁴NO, Ar²²Ne-¹⁵N¹⁴N0, Ar²⁰Ne-¹⁴N¹⁵NO and Ar²²Ne-¹⁴N¹⁵NO. Nuclear quadrupole hyperfine structures of the rotational transitions that are due to the one or two ¹⁴N nuclei were resolved and analysed. The resulting spectroscopic constants were used to provide structural and dynamical information about the trimer. Based on the quartic centrifugal distortion constants, a harmonic force field analysis was performed to estimate the frequencies of the van der Waals vibrational modes. A perturbation of the electronic charge distribution at the site of the central ¹⁴N nucleus of N²0 upon complex formation was detected and discussed. Differences of structural parameters of the trimer as compared to those of the respective dimer units are indicative of the presence of significant three-body non-additive contributions to the interaction energy.     

Development and validation of an HPLC confirmatory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

A high-performance liquid chromatographic method with diode-array detection, at 351 nm, was developed and validated for the determination of five tetracyclines (TCs): minocycline, tetracycline, oxytetracycline, chlortetracycline, and doxycycline in bovine muscle. Samples were macerated with a buffer solution, centrifuged, and purified using Abselut Nexus SPE cartridges. The separation of the examined TCs was achieved on an Inertsil ODS-3 5 microm, 250 x 4 mm analytical column, at ambient temperature. A multistep gradient elution was followed using 0.05 M oxalic acid and CH3CN, at a flow rate of 1.65 mL/min. The procedure was validated according to the European Union regulation 2002/657/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. The results of the validation process demonstrate that the method can be readily applied to European Union statutory veterinary drug residue surveillance programmes. Mean recoveries of TCs from bovine muscle samples spiked at three concentrations (100, 250, and 400 ng/g) were in the range of 98.7-103.3%. Method's LOQ values achieved were 40 microg/kg for MNC, CTC, and DC and 25 microg/kg for OTC and TC. The decision limits (CCalpha) were in the range of 104.7-109.8 microg/kg, while the detection capability (CCbeta) was in the range of 108.4-116.7 microg/kg for all compounds.     

Robust chaos synchronization based on adaptive fuzzy delayed feedback ${\bf{\mathcal{H}}}_{\bf{\infty}}$ control

In this paper, we propose a new adaptive H_￥\mathcal H_\infty synchronization strategy, called an adaptive fuzzy delayed feedback H_￥\mathcal H_\infty synchronization (AFDFHS) strategy, for chaotic systems with uncertain parameters and external disturbances. Based on Lyapunov–Krasovskii theory, Takagi–Sugeno (T–S) fuzzy model and adaptive delayed feedback H_￥\mathcal H_\infty control scheme, the AFDFHS controller is presented such that the synchronization error system is asymptotically stable with a guaranteed H_￥\mathcal{H}_{\infty } performance. It is shown that the design of the AFDFHS controller with adaptive law can be achieved by solving a linear matrix inequality (LMI), which can be easily facilitated by using some standard numerical packages. An illustrative example is given to demonstrate the effectiveness of the proposed AFDFHS approach.     

Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002/657/EC

An HPLC method with diode-array detection, at 355 nm, was developed and validated for the determination of seven tetracyclines (TCs) in milk: minocycline (MNC), TC, oxytetracycline (OTC), methacycline (MTC), demeclocycline (DMC), chlortetracycline (CTC), and doxycycline (DC). Oxalate buffer (pH 4) was used with 20% TCA as a deproteinization agent for the extraction of analytes from milk followed by SPE. The separation was achieved on an Inertsil ODS-3, 5 microm, 250 x 4 mm(2 )analytical column at ambient temperature. The mobile phase, a mixture of A: 0.01 M oxalic acid and B: CH(3)CN, was delivered using a gradient program. The procedure was validated according to the European Union decision 2002/657/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. Mean recoveries of TCs from spiked milk samples (50, 100, and 200 ng/g) were 93.8-100.9% for MNC, 96.8-103.7% for OTC, 96.3-101.8% for TC, 99.4-107.2% for DMC, 99.4-102.9% for CTC, 96.3-102.7% for MTC, and 94.6-102.1% for DC. All RSD values were lower than 8.5%. The decision limits CC(a) calculated by spiking 20 blank milk samples at MRL (100 microg/kg) ranged from 101.25 to 105.84 microg/kg, while detection capability CC(b )from 103.94 to 108.88 microg/kg.