Tagged molecule induced nanoparticle aggregation： Raman reporter-labeled immuno-Au aggregate as immuno-sensor

<正>A novel structure with high surface enhanced Raman scattering(SERS) activity and bio-specificity as a SERS-based immuno-sensor(named as Raman reporter-labeled immuno-Au aggregate) is demonstrated and employed for protein detection.In each fabrication process,the features of those aggregates are obtained and characterized by ultraviolet-visible(UV-Vis) extinction spectra,transmission electron microscopy (TEM) images,scanning electron microscopy(SEM) pictures,and SERS spectra.Experimental results indicate that proper amounts of the reporter molecules can result in the moderate aggregation morphologies of gold nanoparticles.Compared with the previously reported method using Raman reporterlabeled immuno-Au nanoparticles,more sensitive SERS-based protein detection is realized with this novel immuno-sensor.     

Biocompatible and stable core/shell drug nanocarrier with high surface-enhanced Raman scattering activity

<正>A novel drug carrier based on SiO_2-coated silver nanoparticle aggregates and antitumor drug is successfully synthesized.The surface-enhanced Raman scattering(SERS) spectra of the antitumor drug in living cells are obtained.By using silver nano-aggregates as SERS substrates instead of dispersed silver particles, a great improvement of SERS signal intensity is achieved.It is found that the chemical stability of the drug carrier can also be increased with the existence of SiO_2 shell.The adsorbing effect between antitumor drug 9-aminoacridine(9AA) and silver particles is investigated to optimize the SERS signal. The core/shell structure of the drug carrier is characterized by ultraviolet-visible(UV-Vis) spectroscopy and transmission electron microscopy(TEM) pictures.The experimental results show that the drug carrier offers biocompatibility,stability,and high SERS activity,holding the potential for realizing the intracellular drug tracing.     

光纤布拉格光栅法布里-珀罗腔纵模特性研究

分析了光纤布拉格光栅构成的法布里-珀罗（F-P）腔的透射谱特性，讨论了光栅带宽，反射系数相位因子以及腔长对F-P腔透射特性的影响，并对F-P腔随温度和应变的特性进行了分析，然后给出如何设计在光栅中心耦合波长处单模运转的法布里-珀罗腔.并提供了数值模拟结果和实验结果. 关键词： 光纤布拉格光栅 法布里-珀罗（F-P）腔 光纤激光器     

Wide-band double-pass discrete Raman amplifier with reflection of signals and pumps

A wide-band (1530-1610 nm) and high efficient double-pass discrete Raman amplifier is reported. In this Raman amplifier, by using a one-end gilded fiber as the broadband reflector, signals and multi-pump are both reflected to propagate through the gain fiber for a second time. An increase in net gain of more than 150% has been achieved compared with that in the typical co-pumped Raman amplifier. The advantages of this proposed new configuration have been experimentally studied by comparing with the recently existing Raman amplifier configurations.     

基于金纳米聚集体的核壳型SERS探针及其细胞内应用

利用金纳米粒子的聚集体作为表面增强拉曼散射（Surface enhanced Raman scattering,SERS）的增强基底,合成了一种二氧化硅包裹的核壳型SERS探针,并成功将该探针应用于活细胞的SERS光谱探测.实验中利用4-巯基苯甲酸（4-mercaptobenzoicacid,4MBA）作为拉曼标记物,...     

一种信号光与泵浦光同时被反射的宽带双程分立式拉曼放大器的特性研究

对一种高效的宽带（80nm）双程分立式拉曼放大器的特性进行了理论及实验研究。该拉曼放大器中利用一端镀有金膜的光纤作为宽带反射镜，信号光及泵浦光均被反射而第二次经历增益光纤。与传统的前向泵浦的拉曼放大器相比，该拉曼放大器的增益提高了约150％。此外，分析了影响增益的几种因素，包括泵浦间的相互拉曼作用以及光纤的长度。     

Wide-band double-pass discrete Raman amplifier with reflection of signals and pumps

A wide-band(1530-1610 nm)and high efficient double-pass discrete Raman amplifier is reported.In this Raman amplifier,by using a one-end gilded fiber as the broadband reflector,signals and multi-pump are both reflected to propagate through the gain fiber for a second time.An increase in net gain of more than 150% has been achieved compared with that in the typical co-pumped Raman amplifier.The advantages of this proposed new configuration have been experimentally studied by comparing with the recently existing Raman amplifier configurations.     

Stable single frequency Er-doped all-fiber ring laser with fiber Bragg grating Fabry-Perot filter

Using a fiber Bragg grating (FBG) and a Fabry-Perot cavity composed of two fiber Bragg gratings (FBGFP) as its frequency-selective components, a type of single frequency all-fiber ring laser permits oscillation only on one longitudinal mode of the main cavity without modehopping while the cavity length can be up to tens of meters. The salient feature is due to the single narrowband resonance of the FBGFP filter. Such a fiber ring laser is achieved experimentally, and the laser mode is limited inside the single resonance of the FBGFP.     

Interaction between antitumor drug and silver nanoparticles: combined fluorscence and surface enhanced Raman scattering study

Optical methods and MTT method are used to characterize the antiproliferation effect of antitumor drug 9-aminoacridine （9AA） with and without silver nanoparticles. Intracellular surface enhanced Raman scattering （SERS） spectra and fluorescent spectra of 9AA indicate the form of 9AA adsorbed on the surface of silver nanoparticles. Although both silver nanoparticles and antitumor drug can inhibit the growth of Hela cells, silver nanoparticles can slow down the antiproliferation effect on Hela cells at low concentration of antitumor drugs. Our experimental results suggest that silver nanoparticles may serve as slow-release drug carriers, which is important in antitumor drug delivery.     

预聚集法制备单层银纳米粒子膜及其SERS活性研究

提出一种预聚集方法来制备单层银纳米粒子膜, 获得了高活性的表面增强拉曼散射基底. 利用紫外-可见吸收光谱、TEM, SEM等表征手段分析了预聚集程度对银纳米单层膜基底SERS活性的影响. 实验发现该方法制备的银纳米粒子膜的SERS活性与预聚集程度直接相关, 在最优参数下制备的SERS基底具有银颗粒分布均匀、SERS活性均一、增强效果好等优点. 实验分别以罗丹明6G (R6G)、3-巯基丙酸(3MPA)和9-氨基吖啶盐酸盐(9AA)为探针对所制备基底的SERS活性进行了测试, 结果均获得了高信噪比的SERS信号.