Transport Selectivity of a Diethylene Glycol Dimethacrylate- Based Thymine-imprinted Polymeric Membrane over a Cellulose Support for Nucleic Acid Bases

The binding mechanism between 9-vinyladenine and pyrimidine base thymine in methanol was studied with UV-visible spectrophotometric method. Based on this study, using thymine as a template molecule, 9-vinyladenine as a novel functional monomer and diethylene glycol dimethacrylate as a new cross-linker, a specific diethylene glycol dimethacrylate-based molecularly imprinted polymeric membrane was prepared over a cellulose support. Then, the resultantly polymeric membrane morphologies were visualized with scanning electron microscopy and its permselectivity was examined using thymine, uracil, cytosine, adenine and guanine as substrates. This result showed that the imprinting polymeric membrane prepared with diethylene glycol dimethacrylate exhibited higher transport capacity for the template molecule thymine and its optimal analog uracil than other nucleic acid bases. The membrane also took on higher permselectivity than the imprinted membrane made with ethylene glycol dimethacrylate as a cross-linker. When a mixture including five nucleic acid bases thymine, uracil, cytosine, adenine and guanine passed through the diethylene glycol dimethacrylate-based thymine-imprinted polymeric membrane, recognition of the membrane for the template molecule thymine and its optimal analog uracil was demonstrated. It was predicted that the molecularly imprinted membrane prepared with diethylene glycol dimethacrylate as cross-linker might be applicable to thymine assay of absolute hydrolysates of DNA or uracil assay of absolute hydrolysates of RNA in biological samples because of its high selectivity for the template molecule thymine and its optimal analog uracil.     

6-氯-2-氨基嘌呤核苷酸的合成、光谱性质及生化评价的研究

经过4步合成了一个嘌呤核苷酸衍生物6-氯-2-氨基嘌呤核苷三磷酸盐,使用1H NMR、13C NMR、31P NMR、IR和元素分析表征了它的化学结构。在不同pH值的溶液中研究了它的紫外和荧光光谱性质,并通过pH光度微量滴定法测定了一个质子化产物的pKa值为1.44。最后,在马铃薯三磷酸腺苷双磷酸酶的催化下研究了此化合物的水解特性,这个核苷衍生物三磷酸盐与ATP竞争马铃薯三磷酸腺苷双磷酸酶的活性位点的机理被证明是一个混合型抑制机理。     

分子印迹技术研究进展

分子印迹是制备具有分子特异识别功能聚合物的一种技术。本文从分子印迹聚合物的识别机理、分子印迹聚合制备条件和制备技术三个方面综述了分子印迹的研究进展,最后展望了分子印迹发展前景。引用文献66篇。     

二乙二醇二甲基丙烯酸酯交联的胸腺嘧啶分子印迹聚合物纤维素支撑膜对核酸碱基分子选择性研究

使用光度分析法探讨了功能单体9-乙烯腺嘌呤和模板分子胸腺嘧啶在甲醇中的结合作用。在此基础上，以二乙二醇二甲基丙烯酸酯为交联剂，纤维素膜为支持体，制备了分子印迹聚合物膜。通过扫描电镜观察并比较了分子印迹聚合物膜、非分子印迹聚合物膜和纤维素载体膜通道的差异，以胸腺嘧啶、尿嘧啶、胞嘧啶、鸟嘌呤和腺嘌呤为底物，评价了印迹聚合物膜的渗透选择性。结果表明分子印迹膜对模板分子胸腺嘧啶及结构类似物尿嘧啶呈现高的选择性，与用乙二醇二甲基丙烯酸酯为交联剂制备的分子印迹膜比较，也呈现高的扩散速率和选择性，因此该膜对生物样品DNA完全水解产物胸腺嘧啶和RNA水解产物尿嘧啶的识别展现了良好的应用前景。     

亚乙烯基嘌呤核苷衍生物的合成、荧光和生化性质的研究进展

综述了亚乙烯基核苷衍生物(1,N⁶-亚乙烯基腺苷, N²,3-亚乙烯基腺苷, 1,N²-亚乙烯基鸟苷, N²,3-亚乙烯基鸟苷, 1,N⁶-亚乙烯基脱氧腺苷, 1,N²-亚乙烯基-2'-脱氧鸟苷, N²,3-亚乙烯基-2'-脱氧鸟苷)的研究进展, 讨论了其荧光性质和生物活性的应用, 展望了新型荧光扩环核苷衍生物的合成及其在荧光探针和高效药物筛选方面的研究前景.