STUDY ON THE GENOME OF CAULIFLOWER MOSAIC VIRUS (XINJIANG ISOLATE)——RESTRICTION MAPPING OF VIRION AND CLONED VIRAL DNA

The genomie DNA of CaMV (Xinjiang isolate) was mapped on the virion DNA with anumber of restriction endonueleases by double digestions and partial digestions of one-end~(32)P-labelled fragments. It contained the unique sites of BglI, SalI and XhoI. BamHI andHhaI each cut it at two sites, EeoRI at five sites (three of which were located), Hpa Ⅱ atseven sites, BglⅡ at eight sites, and HaeⅢ at ten sites. The sites of all the enzymes usedabove, as well as HindⅢ which cut at ton sites were also mapped on the cloned CaMVDNA. Virion DNA and cloned viral DNA gave the same results in the number and locationof cleavage sites of the restriction enzymes tested on both DNAs. Like most of the otherisolates, CaMV-Xinjiang isolate has three single-stranded discontinuities. From the diges-tion patterns of SalI, XhoI, EcoRI and HhaI, it is concluded that the restriction map ofCaMV-XJ genome is closely related to that of BKMV isolate from East Germany.     

花椰菜花叶病毒(新疆分离物)基因组的研究——病毒粒体DNA和克隆的病毒DNA的限制性内切酶图的测定

本文用混合酶解和一端以~(32)P标记的DNA的部分酶解测定了花椰菜花叶病毒新疆分离物(CaMV-XJ)基因组DNA的限制性内切酶图.它含有BglⅠ,SalⅠ,XhoⅠ的单一切点.BamHⅠ和HhaⅠ各有2个切点,EcoRⅠ有5个切点,HpaⅡ有7个切点,BglⅡ有8个切点,HaeⅢ有10个切点,HindⅢ有10个切点.在病毒粒体DNA和克隆的病毒DNA上这些酶的切点数目和位置是一样的.同其他大多数CaMV分离物一样,CaMV-XJ DNA上也有3个单链不连续区.从SalⅠ,XhoⅠ,EcoRⅠ,HhaⅠ的酶切情况看,CaMⅤ-XJ基因组的内切酶图谱与东德的CaMⅤ分离物BkMⅤ比较接近。     

感染烟草花叶病毒的烟叶无细胞提取物离体合成的RNA产物的性质

从感染TMV的普通烟叶中分离到含有TMV-RNA复制酶的无细胞提取物(20,000×g沉淀),它能在四种三磷酸核苷(其中三磷酸尿苷用氚标记)、镁离子和放线菌素D的存在下离体合成双链RNA,经酚-SDS提取、Serva纤维素柱层析获得的³H-双链RNA,用聚丙烯酰胺-琼脂糖凝胶电泳、核糖核酸酶处理、热变性、自退火等手段,证实³H-双链RNA中含有TMV-RNA的复制型(RF)和复制中间体(RI),分子量分别为4.0×10⁶和5.0×10⁶,分子杂交竞争试验表明³H-双链RNA中新生链的60-70％为TMV-RNA正链。