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DNA was extracted from NIH 3T3 cells transformed with DNAs from human primary hepatic cancer (PHC) and Hepatoma 7402 cell line. The transformant DNA was analyzed by Southern transfer and hybridization with ~(32)P-labeled probes of various oncogenes.The EcoRI 7.2 and 9.0 kb bands characteristic of human N-ras gene were identified in transformed NIH 3T3 cells derived both from PHC and 7402 DNA. The BamHI 6.6kb band characteristic of human c-Ha-ras I was present only in 7402 transformants, but not in PHC transformants.Using ~(35)S-methionine incorporation, immunoprecipitation with anti-p21 monoclonal antibodies, SDS-PAGE and autoradiography, it was demonstrated that p21 synthesis was remarkably enhanced in 1402 cells as well as in transformed cells derived from both 7402 and PHC DNA.Taking the data together, it. strongly implies that N-ras is one of the transforming genes for human liver cancer. 相似文献
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INFLUENCE OF HIGH PRESSURE ON EMISSION SPECTRA AND CRYSTAL FIELD STRENGTH FOR Eu3+ IN GdOBr 下载免费PDF全文
The emission spectra of the Eu3+ion in GdOBr∶Eu3+ were measured in the range of pressure from 0 GPa to 12.4 GPa. The energy level schemes establis hed from the spectra show that the splittings of the 7F1,7F3 and 5D1 multiplets decrease obviously with increasing pressure. The crystal-field parameters Bkq and the crystal-field strength parameter S versusBkq were determined at different pressures. With pressurization S reduces and theJ-mixings of the state functions for the Eu3+ ion decrease, which is in agreement with the decrease of the multiplet splittings. This shows that for Eu3+ in REOX∶Eu3+ the diminution of the crystal-field strength with pressure, the authenticity of which is under suspicion for several years, is certain. 相似文献
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We have measured the synchrotron radiation photoelectron spectra of monolayer C60on Ag(100), By calculating the intensity ratios between the LUMO bands and the two deeper bands (HOMO and HOMO- 1), we estimate the amount of the charges transferred from Ag(100) to C60 within the range of 1 e to 1.8 e. The results dismay the expectation of surface superconductivity and afford a good reference for further studies of the monolayer C60/Ag(100) system. 相似文献
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We have studied the electronic structure of [6,6]-phenyl-C61-butyric-acid-methyl-ester (PCBM) using synchrotron radiation photoelectron spectroscopy (PES) measurements and first-principles calculations. The PES spectrum of the entire occupied valence band is reported, which exhibits abundant spectral features from the Fermi level to ~24 eV binding energy. All the spectral features are broadened as compared with the cases of C60. The reasons for the broadening are analysed by comparing the experimental data with the calculated energy levels and density of states. Special attention is paid to the analysis of the C60 highest occupied molecular orbital (HOMO)-1 derived states, which can play a crucial role in the bonding at the interfaces of PCBM/polymer blenders or PCBM/electrodes. Besides the well-known energy level splitting of the C60 backbone caused by the lowered symmetry, C 2p states from the side chain mix or hybridize with the molecular orbitals of parent C60. The contribution of the O 2p states can substantially modify the PES spectrum. 相似文献
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在C60单晶(111)解理面上制备出厚度约30 nm的K3C60单晶膜.利用同步辐射光源,在低温下(约150K)测量了样品法向发射的角分辨光电子谱.观察到K3C60导带和价带明显的色散.导带的光电子谱峰可清晰分辨出4个子峰,这些子峰的最大色散超过0.5eV,并且色散曲线与K3C60的一维无序晶体结构模型下的能带理论基本吻合,只是子带间隔差异较大. 相似文献
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Photoemission spectra are measured for Yb covered surface of wet-chemically-etched H-Si (111). The results reveal that the lattice structure of the H-Si (111) surface is stable against the deposition of Yb atoms. X-ray photoemission spectra indicate the formation of a polarized (dipole) surface layer, with the silicon negatively charged. Ultraviolet photoemission spectra exhibit the semiconducting property of the interface below one monolayer coverage. Work function variation during the formation of the Yb/H-Si (111) interface is measured by the secondary-electron cutoff in the ultraviolet photoemission spectral line. The largest decrease of work function is ~1.65eV. The contributions of the dipole surface layer and the band bending to the work function change are determined to be ~1.15eV and ~0.5eV, respectively. The work function of metal Yb is determined to be ~2.80±0.05eV. 相似文献
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研究了K3C60单晶薄膜在200K附近的导带结构.样品温度为190K时,同步辐射角分辨光电子谱能够观察到[111]方向有规律的能带色散.而在220K附近色散不存在.这一实验结果与K3C60在200K存在取向相变相符合.用反铁磁Ising模型对实验结果进行了分析.结果表明,K3C60在200K的相变是由低温下的一维无序取向结构转变为200K以上的双取向结构畴与无序分子(约占40
关键词:
3C60')" href="#">K3C60
取向相变机理 相似文献