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超声波对固定化酶活性的影响   总被引:11,自引:0,他引:11       下载免费PDF全文
本文介绍了超声波对固定化酶的影响,同时对作用过程中的影响因素进行了阐述,并探讨了超声波影响固定化酶的可能机理。  相似文献
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一种用于化学分析的新方法——荧光毛细分析法   总被引:6,自引:2,他引:4  
提出了一种新方法--荧光毛细分析法(fluorescence capillary analysis,FCA).FCA法用毛细管做为荧光反应和测定的载体,实现了微量样品的微量分析.本方法特点是操作简便,试样、试剂用量少(<10μL);毛细管及毛细生物反应器可循环使用,测定成本极低,能大大减少环境污染.该研究基于FCA法测定了核黄素、还原型辅酶Ⅰ,基于固定化酶FCA法测定了酒中乙醇,基于固定化核苷酸探针基因FCA法测定了靶核苷酸,都初步得到了满意的结果.FCA法将可以应用到临床医学、药学、生化、染料工业、发酵食品、环境检测等各个领域中.  相似文献
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By the method of IR spectroscopy it is established that the process of sorption of celiase, trypsin, chymotrypsin, streptase, plasminogen, and plasmin by monocarboxylcellulose (the content of COOH groups is 15 wt.%) is mainly identical. The determining role in the mechanism of binding of monocarboxylcellulose with the considered medicinal enzymes belongs to electrostatic interactions with the formation of ionic bonds between the COO groups of the matrix and charged amine groups of protein molecules. It is established that the process of interaction of plasmin with oxidized cellulose takes a more active course than with other investigated enzymes. It is shown that the activity of interaction of the enzymes with monocarboxylcellulose can be evaluated by a change in the relative intensity of the band of stretching vibrations of C=O groups. Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 66, No. 6, pp. 771–774, November–December, 1999.  相似文献
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Summary The electrical properties of rat brain lipids impregnated filtermembranes exposed to the lipophilic ion tetraphenylborate (TPhB) have been studied, using electrical relaxation techniques. The state of TPhB transport system depends on the applied voltage. It was also found that the initial membrane conductance depends on the applied voltage and changes with the concentration of TPhB. Our results are presented in comparison with the electrical properties of BLM exposed to TPhB. To speed up publication, the authors of this paper have agreed to not receive the proofs for correction.  相似文献
5.
Summary The influence of cholesterol on TΦB transport through rat brain lipid model membranes has been studied by performing conductance studies. As a model system was used a rat brain lipid filter supported membrane. The weight percent of cholesterol as referred to total lipids was varied in a wide range. Cholesterol produced an increase of the conductance of membranes induced by the lipophilic anion tetraphenylborate. Moroever, an increase in the cholesterol mole fraction had a strong influence on steady-state I–V characteristics of rat brain lipid model membranes. To speed up publication, the authors of this paper have agreed to not receive the proofs for correction.  相似文献
6.
Laccase catalyzing the oxidation of p-diphenols has been applied in many industrial and biotechnology areas. Immobilized form of laccase has overcome the problem with contamination of the final product. Nevertheless sensitive enzymes immobilized to the matrix can be inactivated by the environmental conditions. The aim of this research was to prepare carrier with improved activity and responsible stability even under extreme reaction conditions. Laccase immobilized through carbohydrate moieties on magnetic hydrazide bead cellulose with a final activity of 0.63 I.U./1 ml of settled carrier confirmed that carriers with oriented immobilized enzyme might be useful in routine biocatalytic applications.  相似文献
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