Enzyme immobilization strategies for the design of robust and efficient biocatalysts |
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Authors: | Jose M Guisan Gloria Fernandez-Lorente Javier Rocha-Martin Daniel Moreno-Gamero |
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Institution: | Instituto de Catalisis, Spanish National Research Council (CSIC), Marie Curie 2, 28049, Madrid, Spain |
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Abstract: | Different strategies for the preparation of efficient and robust immobilized biocatalysts are here reviewed. Different physico-chemical approaches are discussed.i.- The stabilization of enzyme by any kind of immobilization on pre-existing porous supports.ii.- The stabilization of enzymes by multipoint covalent attachment on support surfaces.iii.- Additional stabilization of immobilized-stabilized enzyme by physical or chemical modification with polymers.These three strategies can be easily developed when enzymes are immobilized in pre-existing porous supports. In addition to that, these immobilized-stabilized derivatives are optimal to develop enzyme reaction engineering and reactor engineering. Stabilizations ranging between 1000 and 100,000 folds regarding diluted soluble enzymes are here reported. |
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Keywords: | Immobilization-stabilization of enzymes Multipoint covalent immobilization Modification of immobilized enzyme with polymers Hydrophilic and viscous polymers as enzyme stabilizers |
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