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排序方式: 共有106条查询结果,搜索用时 15 毫秒
1.
Membrane transporters are expressed in various bodily tissues and play essential roles in the homeostasis of endogenous substances and the absortion, distribution and/or excretion of xenobiotics. For transporter assays, radioisotope‐labeled compounds have been mainly used. However, commercially available radioisotope‐labeled compounds are limited in number and relatively expensive. Chromatographic analyses such as high‐performance liquid chromatography with ultraviolet absorptiometry and liquid chromatography with tandem mass spectrometry have also been applied for transport assays. To elucidate the transport properties of endogenous substrates, although there is no difficulty in performing assays using radioisotope‐labeled probes, the endogenous background and the metabolism of the compound after its translocation across cell membranes must be considered when the intact compound is assayed. In this review, the current state of knowledge about the transport of endogenous substrates via membrane transporters as determined by chromatographic techniques is summarized. Chromatographic techniques have contributed to our understanding of the transport of endogenous substances including amino acids, catecholamines, bile acids, prostanoids and uremic toxins via membrane transporters. 相似文献
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基于内源性致香物质和化学计量学的烟草感官评价研究 总被引:1,自引:0,他引:1
采用主成分分析法结合遗传算法和神经网络,建立了基于烟草内源性致香物质的感官质量评价预测模型。利用气相色谱-质谱(GC-MS)技术对超临界萃取-分子蒸馏所得烟草精油中的内源性致香组分进行定性定量分析,汇总各类致香指标后,对其进行主成分分析;以提取所得5个主成分的得分作为输入变量,感官评吸分数作为输出变量,分别使用标准BP神经网络和遗传算法(GA)优化的BP神经网络建立预测模型。对比实验结果表明,GA优化后的模型预测效果更优,其预测值与实验值间的相关系数为0.96,预测均方根误差为1.81,说明GA-BP模型具有更好的拟合能力和预测能力,该模型能有效地预测烟草精油的感官品质。 相似文献
3.
Lili Li Shuangshuang Ma Daijie Wang Long Chen Xiao Wang 《Biomedical chromatography : BMC》2020,34(3):e4773
Lonicerae Japonicae Flos (LJF) is a typical herbal medicine and is used as a functional food. LJF, which has complex chemical compounds, has various biological effects. The global metabolomics, focusing on both the endogenous and exogenous metabolites, have not yet been investigated for LJF in normal healthy rats using LC–MS. In this study, plasma metabolomics was analyzed after the administration of LJF at different time intervals, and the exogenous metabolites were identified. Partial least squares discriminant analysis showed significant differences in chemical content in the dosed rats. Cholic acid, indoleacrylic acid, indolelactic acid, hippuric acid, N-acetyl-phenylalanine, and N-acetyl-serotonin significantly accumulated in the dosed rats. Lysophosphatidylethanolamine and lysophosphatidylcholine content, including plasmalogen, increased. There were 25 components of LJF, including 15 prototypes and 10 metabolites, that were identified. The 15 prototypes included phenolic acids, flavonoids, and iridoids, and their contents decreased with an increase in the administration time. Glucuronidation and sulfation of polyphenols were found for LJF. The exogenous glucuronide and sulfate metabolites—including dihydrocoumaric acid-sulfate, dihydrocaffeic acid-sulfate, dihydroferulic acid-sulfate, apigenin-glucuronide, apigenin-glucuronide-sulfate, isorhamnetin-glucuronide-sulfate, and others—were identified with a neutral loss of 176 and 80, respectively. The metabolic differences found in the study may serve as biomarkers of LJF consumption and promote the understanding of the mechanism of action of LJF. 相似文献
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Dr. Jonathan A. Robson Dr. Markéta Kubánková Tamzin Bond Rian A. Hendley Dr. Andrew J. P. White Prof. Dr. Marina K. Kuimova Prof. Dr. James D. E. T. Wilton-Ely 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(48):21615-21619
A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells. 相似文献
6.
本文构建了一个扩展的南北贸易模型,将北方创新、南方模仿和进口产品隐性技术作为内生变量,分析进口国如何选择模仿策略以最大程度地获得进口产品的内生技术溢出效应.结果表明,进口产品中隐性技术含量会影响南方厂商的模仿策略:进口产品隐性技术含量低时,原始模仿是南方厂商的理性选择;进口产品隐性技术产量较高时,南方厂商的模仿策略取决于模仿的预期边际收益与单位模仿成本的对比关系:模仿的预期边际收益大于单位模仿成本时,内生模仿是南方厂商的最优选择,反之原始模仿. 相似文献
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8.
Guoxing Yin Tingting Niu Ting Yu Yabing Gan Xinyu Sun Peng Yin Haimin Chen Youyu Zhang Haitao Li Shouzhuo Yao 《Angewandte Chemie (International ed. in English)》2019,58(14):4557-4561
Studying numerous biologically important species simultaneously is crucial to understanding cellular functions and the root causes of related diseases. Direct visualization of endogenous biothiols in biological systems is of great value to understanding their biological roles. Herein, a novel multi‐signal fluorescent probe was rationally designed and exploited for the simultaneous sensing of homocysteine (Hcy), cysteine (Cys), and glutathione (GSH) using different emission channels. This probe was successfully applied to the simultaneous discrimination between and visualization of endogenous Hcy, Cys, GSH, and their transformation in living cells. 相似文献
9.
高效液相色谱法分离和测定小麦中的5种内源激素 总被引:3,自引:0,他引:3
建立了高效液相色谱法(HPLC)用于分离和测定小麦中的吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA3)、玉米素(ZT)和水杨酸(SA)5种植物内源激素。经过条件优化,选用甲醇作为样品提取溶剂。然后,经石油醚和乙酸乙酯萃取,经Sep-Pak C18小柱纯化。液相色谱的分离采用Eclipse XDB-C18 (250 mm×4.6 mm, 5 μm)反相色谱柱;流速为1 mL/min;进样量10 μL。检测器波长设置为254 nm; 14.5 min时切换到240 nm; SA洗脱后即18 min时切换回254 nm。流动相A为甲醇,B为乙酸溶液(pH 3.6)。梯度条件为0~7 min, 20%A; 7~10 min, 20%A~28%A; 10~17 min, 28%A; 17~19 min, 28%A~40%A; 19~35 min, 40%A。结果表明,小麦中各激素的分离效果理想,加标回收率达96.9%~98%,相对标准偏差在1.54%~2.29%之间。因此,该方法的建立为快速、准确地分离和测定小麦内源激素提供了可靠的方法。 相似文献
10.
考察内生网络环境下局中人之间的局部策略互动, 网络中的局中人只与直接邻居进行协同对策. 网络生成的过程中, 建立连接的费用是异质的~(具有两种水平), 与采取有效行动的局中人建立连接时执行高水平费用, 与采取风险占优行动的局中人建立连接时执行低水平费用. 在异质连接费用的情形下, 首次较为完整地给出了均衡网络的结构特性和局中人的行动选择, 并分析了费用参数对均衡结果的影响. 相似文献