A Simple Frontal Analysis Equation to Determine the Adsorption Parameters of Solute Molecules on Different Adsorbents

A simple frontal analysis equation to determine the adsorption parameters of solute molecules on different adsorbents was presented. It gives the relationship between the average breakthrough time and the feed solute concentration, and by using its linear form, two important parameters, the thermodynamic equilibrium constant KSL for solute adsorption on the surface of adsorbent and the number nt of total adsorption sites distributed on the surface of adsorbent, can be simultaneously determined. The frontal analyses for some aromatic hydrocarbons on RP-C18 reversed-phase medium, and some protein molecules on RP-C18 reversed-phase, WCX-1 cation-exchange, PEG-400 hydrophobic and Chelating Sepharose Fast-flow separately chelated with Zn^2＋ or Cu^2＋ media, were separately carried out to test this equation and their adsorption parameters KSL and nt were separately obtained. The results show that all these frontal analysis data can be well described by this frontal analysis equation. For all of these frontal analysis systems, their parameters nt can separately approximately keep constant and they are independent of solute molecules used, while their parameters KSL are dependent upon both of the media packed in frontal analysis column and the solute molecules used.     

Control of microwave signals using bichromatic electromechanically induced transparency in multimode circuit electromechanical systems

We theoretically investigate the tunable delay and advancement of microwave signals based on bichromatic electromechanically induced transparency in a three-mode circuit electromechanical system, where two nanomechanical resonators with closely spaced frequencies are independently coupled to a common microwave cavity. In the presence of a strong microwave pump field, we obtain two transparency windows accompanied by steep phase dispersion in the transmitted microwave probe field. The width of the transparency window and the group delay of the probe field can be controlled effectively by the power of the pump field. It is shown that the maximum group delay of 0.12 ms and the advancement of0.27 ms can be obtained in the current experiments.     

Energy loss correction for a crystal calorimeter

Material effect of inner-detectors on the performances of the BESⅢ Electromagnetic Calorimeter (EMC) is investigated. The BESⅢ Time-Of-Flight counters (TOF) have been utilized to improve the energy resolution and detection efficiency for photons after a careful energy calibration. A matching algorithm between TOF and EMC energy deposits is developed, and the effects of beam-related background are discussed. The energy resolution is improved and the photon detection efficiency can be increased by the combined measurement of EMC and TOF detectors.     

阴极溶出伏安法测定农药厂废水和癌症患者尿中硫脲(TU)

硫脲的用途很广,但有致癌性。目前国内外采用阴极溶出法测定硫脲的报导很少。Smyth和Osteryoug采用脉冲阴极溶出法测定了农药和尿中1ng/ml的硫脲。但对于如何排除S~(2-)对硫脲的干扰文中并未提及。本文使用普通极谱仪,先后比较了不同碱性溶液中测定硫脲的灵敏度,最后选定了二乙胺为最佳底液。在该底液中硫脲的测定灵敏度较高,线性范围较宽。S~(2-)的干扰用通氮吹气法排除。本法适用于水和尿中硫脲的测定。     

利用BESⅢ的双层TOF寻找D~0-■混合的研究(英文)

在ψ(3770)处,D0→K-π+是研究D0-■混合的非常理想的衰变道．实验上,良好的K／π识别技术将对寻找D0-■混合过程起着决定性的作用．在BESⅢ实验的物理预研究中,发现利用飞行时间的信息,能够精确测定末态中含有多条带电径迹事例的起始时间,从而可以改善飞行时间计数器的时间分辨率．进一步的研究表明,应用该方法后,BESⅢ双层TOF的时间分辨率从～78ps降到～64ps．按照20fb-1的ψ(3770)数据量进行估算,在95％置信度下,D0-■混合率的上限值可以提高7％左右．     

应用随机寡核苷酸文库修饰磁性颗粒提高血浆蛋白质的鉴定覆盖度

基于随机寡核苷酸与蛋白质之间的离子、亲和、疏水、氢键等相互作用力及多种空间结构作用,发展了一种基于随机寡核苷酸文库作为配基的新型血浆样品处理方法。采用寡核苷酸文库修饰的磁性颗粒(MNP@ssDNA)材料,在生理缓冲体系条件下捕获血浆样品中的蛋白质。比较了两种洗脱体系的洗脱效果,并利用nano-RPLC-ESIMS/MS对获得的蛋白质酶解液组分进行分析。结果表明,MNP@ssDNA材料处理后的血浆蛋白质鉴定数量提升了约29.5%,两种洗脱体系呈现良好的互补性(26.7%)。血浆中前10种高丰度蛋白质的谱图占有率从处理前的31.82%降低到21.31%(洗脱体系1)和26.20%(洗脱体系2)。在鉴定到的蛋白质中,丰度最低的蛋白质在血浆中的质量浓度约为0.29 ng/mL,该蛋白质仅在MNP@ssDNA材料处理后被鉴定到。结果证明MNP@ssDNA策略不仅能有效降低血浆中高丰度蛋白质的丰度,也为低丰度蛋白质的深度挖掘提供了新的思路。     

荧光相图法研究猪胰腺α-淀粉酶在脲和盐酸胍溶液中的去折叠过程

用荧光相图法分别研究了脲和盐酸胍诱导的猪胰腺α-淀粉酶的去折叠过程。实验结果表明,当脲作为变性剂时,无论变性体系中有无还原剂2-巯基乙醇存在,猪胰腺α-淀粉酶的去折叠过程均只出现一个部分折叠中间体,符合“三态模型”;当盐酸胍作为变性剂时,若变性体系中存在还原剂2-巯基乙醇,猪胰腺α-淀粉酶的去折叠过程符合“三态模型”,而若变性体系中不存在还原剂2-巯基乙醇,猪胰腺α-淀粉酶的去折叠过程会出现两个部分折叠中间态,此过程符合“四态模型”。     

从猪血中分离纯化高纯度的猪血红蛋白

为了从猪血中分离纯化高纯度的猪血红蛋白,建立了通过超滤、DEAE-Sepharose Fast Flow离子交换色谱和Sephadex G-75凝胶 排阻色谱三步法制备高纯度猪血红蛋白的方法,并通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效凝胶排阻色谱和反相高 效液相色谱方法,对纯化后的猪血红蛋白进行了鉴定。经三步分离纯化后,猪血红蛋白的纯度大于99%,含量为1.328 g/L。     

Propagation of electromagnetic wave in the three phases soil media

IntroductionItisofparamountimportancetogetgeotechnicalparameterssuchasmoisture ,compactness,andshearstrengthforgeotechnicalengineeringtransaction .Butthemethodtofetchthesoilsamplesforlaboratorytestcannotavoidbringingthedisturbance ,andmakethetestresultsinconsistenttotheactualstatusofsoil.Inrecentyears,theapplicationofEMwaveinthedetectinghiddentroublesindamsandundergroundfoundationsdevelopedrapidly .Buttherearestillsomefactorsthatrestricttheadvanceofapplication .Firstly ,thesoilconstitutesthr…     

一种从蛇毒中纯化神经生长因子的新工艺

为了能从中华眼镜蛇蛇毒中简单快速地分离纯化神经生长因子（一种治疗各种神经性损伤和神经退行疾病的药物,简称NGF）,采用不同的色谱柱联用的方式对NGF的纯化工艺进行了研究。结果表明,采用DEAE Sepharose F.F.和Sephadex G 50二步柱色谱工艺可以从蛇毒中快速分离得到神经生长因子。经十二烷基硫酸钠聚丙烯酰胺凝胶电泳和反相高效液相色谱分析, 证明所得到的NGF为单一组分,相对分子质量约为 29 000。对8　d龄鸡胚背根神经节采用体外培养法,结果证明,所得NGF具有明显的促进神经纤维