化学发光酶免疫分析法检测水产品中残留的麻醉剂丁香酚

将丁香酚(Eul)与4-溴丁酸叔丁酯进行衍生化反应,合成了半抗原4-(4-烯丙基-2-甲氧基-苯氧基)-丁酸(Eul-4-Tbl).通过偶联载体蛋白并免疫动物后获取丁香酚多克隆抗体,建立了丁香酚的间接竞争化学发光酶免疫分析方法(ic-CLEIA).水产样品经乙腈提取,Oasis PRiME HLB固相萃取小柱净化后用于...     

高中数学专题研究的一个案例──关于沪闵路─春申路口红绿灯时间设置合理性的研究

一、专题的背景与分析 　　1. 背景 　　闵行区的沪闵路─春申路口是交通特别拥挤的交叉路口之一.家住莘庄地区的同学有一个共同的感受,在他们到校或回家路上必经的沪闵路─春申路口时常遇到塞车现象.……     

Vibrational analysis of L-serine using the density functional theory

In this paper, we present a computational study of L-serine using ab initio molecular dynamics simulation based on density functional theory (DFT) within the ultrasoft pseudopotentials and generalized-gradient approximation. Taking into account the intermolecular interactions, we can indeed simulate the features of the experimental results very well for L-serine zwitterions in its solid state. The vibrational spectrum of L-serine performed by DFT was in excellent agreement with our previous inelastic incoherent neutron scattering spectra measured at 20K for L-serine in the 10--200meV region on HET spectrometers at ISIS, Rutherford Appleton Laboratory.     

薄层色谱法在蔗糖-6-苯甲酸酯合成过程中的应用

用薄层层析法对蔗糖－６－苯甲酸酯合成过程中所涉及的反应物进行分离分析，选择 经出最佳展开剂体系为：四氢呋喃：水：乙酸＝４：６：５：０．５（ｖ／ｖ）。在该展开剂条件下，可以有效地分离反应原料、反应中间体和反应产物，较直观地指标了反应里程。     

纳米铜修饰电极对多巴胺和抗坏血酸的同时测定

用电沉积方法制备了纳米铜修饰电极并将其用于混合溶液中多巴胺(DA)和抗坏血酸的同时测定。在优化的实验条件下,修饰电极对多巴胺和抗坏血酸具有良好的电催化响应,多巴胺的峰电流与浓度在8.0×10-7mol/L~1.0×10-4mol/L范围内成很好的线性关系,抗坏血酸的氧化峰电流与其浓度在8.0×10-6mol/L~1.0×10-3mol/L的范围成良好的线性关系。该修饰电极制备简单、稳定性好,用于样品检测,效果良好。     

第二届全国激光新晶体材料学术会议简讯

由中国光学学会和中国硅酸盐学会共同组织的第二届全国激光新晶体材料学术会议于1983年11月25-29日在泉州市华侨大学陈嘉庚纪念堂举行.通过46篇学术论文的交流和讨论,反映出我国近三年来激光与非线性光学新晶体研究工作的蓬勃发展. 激光晶体的研究重点是:1.终端声子跃迁的可调谐激光晶体,主要有Cr～(3+):BeAl_2O_4和Ni2+:MgF_2。通过晶格动力学与晶格场理论相结合的分析探索新的基质晶体;2.稀土自激活小型激光晶体.积极研制跃迁通道丰富的HO～(3+),Er～(3+)激活晶体.对TbxDy_(1-N);P_5O_(14),Er-x(Gd,Y)1_(1-x)Al_3(BO_3)_4LiNd_(…     

全国激光新材料协作交流会在厦门市举行

由国家科委光学及应用光学学科组光学材料分组和中国光学学会光学材料委员会倡议组织的全国激光新材料协作交流会于1980年11月15日至20日在厦门市举行。从事激光晶体新材料研究工作的科研单位和大专院校都派代表参加了会议。与会代表们听取了8篇邀请报告后,进一步交流了在激光新材料研究方面的情况和各单位近期计划。 北京华北光电所、中国科学院物理所、上海光机所和天津硅酸盐所等单位曾用提拉法生     

Studies on Long-acting Insulin:Crystal Structure of Arg-B31 Human Insulin at 2.0 Resolution

The crystal structure of Arg-B31 human insulin(ABHI), a long-acting insulin derivative, has been determined at 2.0 resolution by using X-ray diffraction analysis. The final crystallographic R factor of the structure model after the refinement is 0.189 with the bond length r. m. s deviation of 0.018 . The refined structure of ABHI showed that the conformation of B-chain C-terminal residues was more stable than that in the native molecule. A striking structural feature of ABHI was an additional ion pair formed between ArgB31 of molecule Ⅰ and Glu-B21 of molecule Ⅱ in a dimer, and three ionic bonds between the neighbouring molecules thereby appeared on the surface of ABHI hexamer.These secondary bonds generated by the insertion of the residue Arg-B31 should make the rate of dissociation of ABHI hexamer slow down when it was injected into the body and the property of protraction should be produced by a 'depot effect'. This ought to be the main structure basis of the prolonged action of ABHI. The results o     

Highly efficient fiber-to-chip evanescent coupling based on subwavelength-diameter optical fibers

A novel, compact, and highly efficient fiber-to-chip evanescent coupling structure is proposed based on a subwavelength-diameter fiber. The coupling structure is characterized by a large misalignment tolerance and easy fabrication. The dependence of coupling efficiency on various parameters is calculated and analyzed. The simulation results show that a coupling efficiency as high as 95% can be obtained within a coupling length of <4 μm.     

紫外光谱法测定饮料中的咖啡因含量

建立了一种测定饮料中咖啡因含量的紫外光谱分析法.采用三氯甲烷为萃取剂,控制三氯甲烷与饮料的体积比为8:1,经充分振荡后离心分离,取清液在276 nm处测定紫外吸收光谱,能够满意地测定市售饮料中的咖啡因浓度.分析结果的相对标准偏差小于4%;在饮料中加入不同浓度的咖啡因标准溶液,回收率在94.0%～112.0%之间.