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71.
72.
海藻酸钠固定化根霉脂肪酶的制备及其性质 总被引:10,自引:0,他引:10
研究了以海藻酸钠为载体,用包埋法制备固定化德氏根霉(Rhizopus delemar)脂肪酶的条件. 将酶粉和海藻酸钠溶于pH 5.0的HAc-NaAc缓冲溶液,用注射器将此混合液滴入到0.05 mol/L无菌CaCl2溶液中,静置固化45 min, 经过滤、洗涤和干燥后得到球状固定化酶. 固定化酶的活力回收约为34.1%. 酶学性质研究表明,此固定化酶的热稳定性较好. 游离酶在 60 ℃下保温1 h已完全丧失活力,而固定化酶在100 ℃下保温1 h仅损失36.2%的活力,在100 ℃下保温6 h仍可保持46.8%的酶活力. 酶经固定化后,其橄榄油水解反应的最适温度由40 ℃上升至90 ℃, Km值由13.8 mg/ml下降为8.1 mg/ml. 常见有机溶剂对固定化酶的活力影响较小. 将该固定化脂肪酶用于非水溶剂中正戊酸异戊酯的合成,重复使用6次后,固定化酶仍保持95%的酶活力. 相似文献
73.
Gaffney Alexea M. Markov Sergei A. Gunasekaran M. 《Applied biochemistry and biotechnology》2001,91(1-9):185-193
The effectiveness of photosynthetic free-living and polyurethane foam (PU) immobilized Anabaena variabilis cells for, removal of orthophosphate (P) from water in batch cultures and in a photobioreactor was studied. Immobilization
in PU foams was found to have a positive effect on P uptake by cyanobacteria in batch cultures. The efficiency of P uptake
by immobilized cells was higher than by free-living cells. A laboratory scale photobioreactor was constructed for removal
of P from water by the immobilized cyanobacteria. The photobioreactor was designed so that the growth medium (water) from
a reservoir was pumped through a photobioreactor column with immobilized cyanobacteria and back to the reservoir. This created
a closed system in which it was possible to measure P uptake. No leakage of cells into the photobioreactor medium reservoir
was observed during the operation. The immobilized cells incorporated into a photobioreactor column removed P continuously
for about 15 d. No measurable uptake was demonstrated after this period. Orthophosphate uptake efficiency of 88–92% was achieved
by the photobioreactor. 相似文献
74.
Wong Mui F. Chua Hong Lo Waihung Leung Chu K. Yu Peter H. F. 《Applied biochemistry and biotechnology》2001,91(1-9):447-457
Studies were conducted toinvestigate the removal and recovery of copper (II) ions from aqueous solutions by Micrococcus sp., which was isolated from a local activated sludge process. The equilibrium of copper biosorption followed the Langmuir
isotherm model very well with a maximum biosorption capacity (qmax) of 36.5 mg of Cu2+/gofdry cell at pH 5.0 and 52.1 mg of Cu2+/g of dry cell at pH 6.0. Cells harvested at exponential growth phase and stationary phase showed similar biosorption characteristics
for copper, Copper uptake by cells was negligible at pH 2.0 and then increased rapidly with increasing pH un til 6.0. In multim
etal systems, Micrococcus sp. exhibited a preferential biosorption order: Cu−Pb>Ni−Zn. There is virtually no interference with copper uptake by Micrococcus sp. from solutions bearing high concentrations of Cl−, SO
4
2−
, and NO3/− (0–500 mg/L). Sulfuric acid (0.05 M) was the most efficient desorption medium, recovering >90% of the initial
copper sorbed. The copper capacity of Micrococcus sp. remained unchanged after five successive sorption and desorption cycles. Immobilization of Micrococcus sp. in 2% calcium alginate and 10% polyacrylamide gel beads increased copper uptake by 61%. Biomass of Micrococcus sp. may be applicable to the development of potentially cost-effective biosorbent for removing and recovering copper from
effluents. 相似文献
75.
Daniela Šmogrovičová Zoltán Dömény Juraj Švitel 《Applied biochemistry and biotechnology》2001,94(2):147-158
Immobilized beer fermentation was studied using an industrial bottom-fermenting yeast strain Saccharomyces cerevisiae. The yeast cells were immobilized in 2.5% calcium alginate gel and used for brewing in a five-vessel cascade reactor. The
fermentation was performed at 15°C at various flow rates. A nonstructured mathematical model was developed to simulate the
performance of continuous primary fermentation of lager beer. The model was based on the following variables: maltose, maltotriose,
glucose, fructose, ethanol, and cell concentration. Experimental values of these variables were determined in samples taken
at regular intervals. For experimental data fitting a nonlinear regression was used. Substrate consumption was characterized
by specific substrate consumption rate and saturation constant. The values of these two parameters were optimized for all
four substrates. Inhibition effects of substrates and product were analyzed using various inhibition patterns. Only the inhibition
effect of maltose on maltose consumption was clearly identified. A good-fitting relationship for maltose inhibition was found,
and inhibition constants were calculated. 相似文献
76.
Humic substances are natural complexed mixtures of organic compounds originated from the decomposition of plant and animal residues. These compounds are ubiquitous in soils, sediments, surface waters and groundwaters. They contain both hydrophobic and hydrophilic moieties, able to interact with hydrophobic organic contaminants and with heavy metals. These sorption interactions play a crucial role in contaminants fate and transport and their understanding and quantification are essential for modeling and predictions. However, sorption analyses frequently suffer from experimental problems. A novel idea presented in this study is to use sol-gel as an inert matrix to immobilize (entrap) specific, well defined, humic molecules which then be used in sorption studies. We developed a successful procedure for the immobilization of humic acid (HA) in a sol-gel matrix. After gelation and drying, the doped gel was crushed and washed several times, yielding a very stable product. It was then used in a series of batch experiments, studying the sorption of several polycyclic aromatic hydrocarbons (PAHs) with Aldrich HA. The sorption coefficients (K
oc) obtained with the immobilized HA were highly correlated with the values expected based on the hydrophobicity of the contaminants. We concluded that the entrapped HA retained its original properties and that it was accessible to the external contaminants through the pore network. 相似文献
77.
The unfolding of α-chymotrypsinogen covalently immobilized on silica beads has been studied by differential scanning calorimetry
(DSC). The enzyme undergoes an unfolding transition which, unlike the free protein, cannot be approximated by a single two-state
process. After immobilization, the unfolding is characterized by the presence of two partially overlapping transitions, both
of them show two-state behavior. The two processes correspond to the separate unfolding of the two domains of the α-chymotrypsinogen
molecule. The loss of cooperativity behavior is a consequence of the covalent immobilization. The two domains showed different
thermal stability as functions of pH. One of them unfolded with a transition temperature T
m2 higher than T
m of the free enzyme, implying stabilization effect of immobilization. However, below pH 4.5, its native structure is lost.
The other transition shows a remarkable pH-independent thermal stability from pH 2.5 to 7.0.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
78.
Chengming Wang Toshiyuki Nagahashi Koichi Aoki Jingyuan Chen 《Journal of Electroanalytical Chemistry》2002,530(1-2)
The conductivity, κ, in a suspension of polystyrene sulfonic latex without supporting electrolyte showed a linear dependence on the volume fraction, vf, of the latex for vf<0.03 with a finite intercept. In contrast, this deviated upward from the linear line for vf>0.03. These variations were qualitatively consistent with the dependence of the voltammetric reduction current of H+ on vf without supporting electrolyte. The current values were only a few percent of the theoretical diffusion-controlled current that could be observed in the suspension with supporting electrolyte. This fact indicates the electrostatic immobilization of the hydrogen ions by sulfonic latex particles. A plot of the current against κ at common values of vf showed that the current for vf>0.07 was smaller than the value predicted from the conductivity. This can be explained in terms of a combination of the increase in electrostatically unbounded H+ estimated by conductance measurements and electric migration in which the electrochemical depletion of [H+] also causes the depletion of the latex. 相似文献
79.
A polymeric scaffold with excellent swelling properties in organic and aqueous environments is highly desirable for the medicinal chemist. Here, we demonstrate that a cross-linked polyacrylamide hydrogel that displays large swelling properties in both organic solvent and water can serve as a scaffold for the photosensitizer hematoporphyrin. Upon exposure to light, the resulting resin efficiently generates singlet oxygen which can then react with appropriate substrates. 相似文献
80.