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71.
In this paper, the utility of ion-exchange displacement chromatography for the concentration and enrichment of trace proteins is examined. Separations with varying displacer concentrations (1–25 mM neomycin sulfate) indicate that higher concentrations result in elevated protein concentrations, at the price of reduced yields. The results demonstrate that displacement chromatography carried out at relatively low displacer concentrations (2.5 mM) can produce significant concentration (8.5-fold) and enrichment (18-fold) of trace proteins present in the feed. Parametric simulations using the steric mass action model are carried out to investigate the concentration effects and enrichment factors observed over a wide range of feed, displacer and buffer counter-ion concentrations, and solute separation factors. The simulations confirm that trace components can be readily concentrated and enriched by displacement chromatography and that these effects will be more pronounced as the separation factor between trace and abundant components is increased. The results presented in this paper indicate the potential of displacement chromatography for improved separations where trace enrichment is critical such as proteomic applications. 相似文献
72.
Quantification of the velocity acceleration factor for colloidal transport in porous media using NMR
Sarah A. Creber Thomas R.R. Pintelon Michael L. Johns 《Journal of colloid and interface science》2009,339(1):168-174
Nuclear magnetic resonance (NMR) techniques were used to quantify the transport of colloids through porous media. This was achieved via the application of chemically-resolved pulsed field gradient (PFG) methods, hence probing the displacement (probability distribution) propagators of both the colloidal and continuous liquid phase. A dilute decane-in-water emulsion was used with flow through a random glass sphere packing being considered. The acquired propagators allowed for quantification of both colloidal entrapment and the velocities of both the continuous phase and the flowing colloids. The flowing colloids were found to experience a velocity acceleration factor (VAF) increase of 1.08 relative to the continuous phase. This was found to be independent of displacement observation time or flowrate. It was speculated to be a consequence of radial exclusion due to the finite size of the colloids. Simulations of the colloidal transport were also performed using a lattice Boltzmann platform and a Lagrangian particle-tracking algorithm which incorporated colloidal radial exclusion. Reasonable agreement was observed between the simulation and the experimental data. 相似文献
73.
Self-mixing interferometry (SMI) has been used to measure the distance and displacement. Although the principle of self-mixing interference is different conventional interference, we concluded that FFT analysis technique could also be used to detect the signal phase and increase the measurement precision of self-mixing interferometry.First the SMI signal is obtained by feeding the light from the target to cavity of laser diode, then it is pre-processed by an analog subtraction circuit to remove the overlapped output intensity due to the injection current modulation. Finally, SMI signal is analyzed by FFT phase detection method. Theoretical analysis and simulation calculations are presented. Experimentally, displacement of a PZT-driven target was measured with a precision of λ/50. 相似文献
74.
多路CCD位移传感器用于路面纵断面检测研究 总被引:8,自引:4,他引:4
本文提出了一种运用多路电荷耦合器件(CCD)位移传感器快速检测路面纵断面的测量系统,介绍了测量原理,给出了数据处理方法和实验结果.此系统设计时速最高为60km/h,路面高度分辨率为0.5mm,检测范围为±120mm.实验证明,此系统用于路面纵断面快速检测是可行的. 相似文献
75.
The possibilities of the radio-frequency amplitude modulation of a low-coherence source for distance and displacement measurements are discussed. This intensity-based method, used within a Michelson interferometer, is a novel alternative to perform dynamic deformation measurements. The theoretical background is presented, together with the experimental verification of the principle. Besides, the results of the first quasi-dynamic tests are shown and the perspectives of the technique discussed. This method is specially useful for the dynamic monitoring of civil structures, where large measurements bases are needed. Furthermore, the application of the amplitude modulation for distance measurements is demonstrated experimentally. 相似文献
76.
In this paper, a dual-beam normal incidence polarisation interferometer is proposed to measure the flying height or head–disk spacing. Using this interferometer, the head–disk spacing can be measured both in magnetic real disk condition and in glass disk condition. It has the advantage of both the currently popular intensity interferometry method and the oblique incidence polarisation interferometry method. With this polarisation interferometer, not only the flying height can be measured down to contact without losing accuracy, but the pitch and roll of the head-slider can also be detected dynamically. The optical parameters of the head-slider can also be determined. Design details and experimental study are presented. 相似文献
77.
78.
In this paper, the resonance frequency equation and expression of displacement amplitude magnifications of a full-wave barber ultrasonic horn are obtained. By discussing the relationships between the displacement amplitude magnifications and the geometrical dimensions, the optimized design of the horn for the largest magnification is proposed, which is helpful to improve the radiation power and the transfer efficiency of the acoustic energy of the ultrasonic oscillatory system. Based on the optimized design of the horn, we introduced a barbell ultrasonic transducer operated in the longitudinal full-wave vibrational model and obtained the resonance frequency equations. For comparison, the resonance frequencies of the full-wave barbell horn and the full-wave barbell transducer are also analyzed by finite element method (FEM). It is shown that the values obtained by theoretical analysis and FEM are in good agreement with experimental observations. We hope that the research of this paper is helpful for the use of the barbell horn and transducer in the applications such as ultrasonic liquid processing. 相似文献
79.
In this paper, a numerical approach for analyzing interacting multiple cracks in infinite linear elastic media is presented. By extending Bueckner’s principle suited for a crack to a general system containing multiple interacting cracks, the original problem is divided into a homogeneous problem (the one without cracks) subjected to remote loads and a multiple crack problem in an unloaded body with applied tractions on the crack surfaces. Thus, the results in terms of the stress intensity factors (SIFs) can be obtained by considering the latter problem, which is analyzed easily by means of the displacement discontinuity method with crack-tip elements proposed recently by the author. Test examples are given to illustrate that the numerical approach is very accurate for analyzing interacting multiple cracks in an infinite linear elastic media under remote uniform stresses. In addition, the displacement discontinuity method with crack-tip elements is used to analyze a multiple crack problem in a finite plate. It is found that the boundary element method is also very accurate for investigating interacting multiple cracks in a finite plate. Specially, a generalization of Bueckner’s principle and the displacement discontinuity method with crack-tip elements are used to analyze multiple circular arc crack problems in infinite plate in tension (including: Two Collinear Circular Arc Cracks, Three Collinear Circular Arc Cracks, Two Parallel Circular Arc Cracks, Three Parallel Circular Arc Cracks and Two Circular Arc Cracks) in a plane elasticity plate. Many results are given. 相似文献
80.
Following our previous study on distorted to non-distorted displacive phase transformation in the two-dimensional square lattice based on the continuous displacement cluster variation method (CDCVM) within the Bragg–Williams (BW) approximation, we performed a higher order approximation, four-body approximation, in the entropy term and compared the results obtained by the two approximations. The transformation temperature decreases with the higher order approximation, which shares the common feature with conventional CVM studies on replacive transformations. The present study predicts the first-order transformation, which is markedly different from the previous study based on the BW approximation. Furthermore, by employing the four-body approximation, we are able to reproduce the o-type distribution of displaced atoms around a Bravais lattice point by changing the atomic interaction energy, which was by no means possible by the BW approximation. 相似文献