重组酶聚合酶扩增:扩增原理及性能分析

脱氧核糖核酸(DNA)放大技术对于核酸检测(NAT)至关重要. 聚合酶链式反应(PCR)虽然是核酸检测的基准扩增技术, 但其主要适用于条件较好的中心实验室. 重组酶聚合酶扩增(RPA)是一种非常有潜力的等温扩增技术, 对仪器设备依赖性小, 适用于资源贫乏地区. 因此, 该技术在核酸检测时不受实验场所限制, 非常适合即时检测(POCT). 作为一种正在快速发展的扩增技术, RPA也存在阻碍其进一步发展的缺陷. 本文对RPA的扩增原理和扩增性能进行了总结, 重点讨论了对扩增性能至关重要的引物重组和ATP动态平衡调控过程, 并概述了RPA存在的缺陷和潜在的解决方向.     

α－混合过程条件密度的估计及其性质

本文在{Xr,t∈N）是一个严平稳过程的假设下,用核估计的方法对未来状态XN＋T的条件密度进行估计．在假设{Xt,t∈N）是α-混合过程的情况下,讨论了过程有限维密度核估计的期望与方差,以及过程条件密度核估计的偏及均方误差．在一定条件下,证明了估计的弱收敛性．     

基于偶氮化反应构筑无酶无试剂型过氧化氢传感器的研究

近年来,人们在临床诊断、化学医药、环境保护等领域对过氧化氢的测定开展了广泛研究~([1]).电化学方法测定H_2O_2具有方法简便、灵敏度高等优点.发展无酶无试剂型H_2O_2传感器是重要的研究方向之一~([2,3]).利用偶氮4-氨基苯硫酚(ATP)膜具有强的导电性,偶氮基可与硫堇(Th)进行偶联反应,从而在电极表面实现Th的共价固定,建立了新型无酶无试剂H_2O_2生物传感器.     

Reaction Mechanism of the Multi-channel Decomposition Reactions of C_(10)H_(14)~+·

n-Butylbenzene cations C10H14^＋ serve as a model compound to investigate the reaction mechanisms of alkylbenzene cations. The reactions of C10H14^＋. decomposition reaction system have been studied extensively at the B3LYP/6-311＋＋G^＊＊ level with Gaussion98 package. The chain reaction of C10H14^＋ dissociation was initiated by C-H bond rupture. All reaction channels initiated by C-H rupture were fully investigated with the vibrational mode analvsis to confirm the transition states and to reveal the reaction mechanism. A theoretical investigation on the reactions of this positive ion free radical can help us fully understand the decomposition processes.     

Laser-diode-pumped zigzag slab Nd:YAG master oscillator power amplifier

A high-repetition rate master oscillator power amplifier pumped with laser diodes (LDs) is reported. An injection seeding single-frequency electro-optical Q-switched Nd:YAG laser is used as an oscillator, and a conductively cooled Nd:YAG zigzag slab with a bounce-pumped architecture is utilized as a power amplifier. Pulse energies of over 800 mJ at 1 064 nm and 400 mJ at 532 nm, corresponding to average powers of 200 and 100 W, respectively, are achieved with a 12.6-ns pulse width at 250 Hz. Output frequency fluctuations and single-frequency operation are further monitored. Experimental results reveal that the proposed system, which features a single-pass amplified configuration, is a promising design for space-based applications.     

磷酸锆类层柱催化剂的制备、表征及其催化甲醇氧化羰基化反应性能

以层状α－磷酸锆为插层主体,采用高温固相离子交换插层法制备Ｃｕ＾２＋,Ｋ柱撑的磷酸锆类层柱催化剂,并用ＸＲＤ,ＩＲ和ＴＧ等方法对其进行了表征。结果表明,金属离子柱撑的α－磷酸锆类层柱催化剂的层间距由原来的０．７６ｎｍ扩撑至０．８１ｎｍ,结晶度有所下降。     

用于激光聚变驱动器的全光纤、全固化光脉冲产生系统

报道一种全固化、全光纤的用于高功率激光驱动的惯性约束聚变驱动器的光脉冲产生系统,采用单纵模振荡器输出连续激光信号,经过相位调制器和振幅调制器,得到一个时间波形上已整形且具有一定带宽(约0.1 nm)的激光脉冲,经光纤放大器放大并经光纤分束器分束后同时输出四路激光脉冲,各路激光脉冲先通过时间同步调整单元精确控制时间同步关系后,经可编程光纤衰减器调节各路之间的功率平衡后再通过光纤放大器做进一步放大并通过150 m光纤传输输出至预放系统.该光纤系统可输出0.3—20 ns、带宽0.1 nm、能量数纳焦的几乎任意 关键词： 激光聚变驱动器 前端 光纤激光系统     

纳米ZnO和ZnO-SiO2复合薄膜的光学性质研究

通过溶胶凝胶(sol-gel)法分别在玻璃衬底上制备了ZnO纳米薄膜和ZnO-SiO2纳米复合薄膜,并利用紫外.可见光分光光度计对薄膜的光学性能进行了分析.可见光一紫外透射谱显示,随着Zn0溶胶浓度从0.7 mol/L降低到0.006 mol/L,制备的ZnO薄膜从只出现一个380 nm(对应的光学禁带宽度为3.27 eV)左右的吸收边到在380和320nm(对应的光学禁带宽度为3.76 ev)左右各出现一个吸收边,并且随着ZnO溶胶浓度的降低,在380-320 nm波段内的透过率明显提高.而ZnO-SiO2复合薄膜只在310 nm左右出现一个吸收边.SiOO2的包覆宽化了ZnO的禁带宽度,包覆后的禁带宽度可达到3.87 ev.     

用独立圈对图的一个划分

图G包含4k个点,k≥2,如果σ_2(G)≥4k,则G包含k-2个4-圈和一个8-圈,并且这k-1个圈点不相交.     

Two-Dimensional Borane with ‘Banana' Bonds and Dirac-Like Ring

Designing new two-dimensional(2 D) semiconductors with novel topological characters is highly desirable for further material innovation. We propose a theoretical design of a stable 2 D inorganic material, namely, borane,which is jointly stabilized by traditional B–B localized and unique B–H–B delocalized chemical bonds. In borane,the bonding natures along different directions are distinguishing, which lead to huge differences in mechanical strengths of 142.73 and 97.47 N/m for a and b directions, respectively. In a unit cell, each hydrogen atom binds to two boron atoms forming a three-center-two-electron(3 c-2 e) bridge bond B–H–B. This can be considered as an extension of diborane molecules from 0 D to 2 D. The collaboration of localized and delocalized chemical bonds endows borane with high structural stability, as indicated by its favorable cohesive energy, high mechanical strength, absence of imaginary modes in the phonon spectrum, and moderate melting point. Remarkably, borane has a fascinating electronic property featured with a Dirac-like ring in the electronic band structure. The unique bonding nature and electronic property in borane would attract intensive interests in both theory and experiment.