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21.
在钣金零件视觉测量中,大尺寸零件超出图像采集摄像机的视场范围,不能一次性获得该零件的完整图像,需要将存在一定重叠区域的图像进行图像拼接。针对特征稀少、灰度信息少、轮廓对称的钣金零件图像不易拼接问题进行了分析和研究,提出了一种在待拼接图像中选择包含重叠部分的等大小区域作为待配准区域,并对其提取轮廓,然后对轮廓图利用相位相关法求待配准区域的配准参数,再根据待配准区域图像与待拼接图像的坐标关系转换求待拼接图像的配准参数并对图像进行配准,实现钣金零件图像拼接的方法。通过实验表明,该拼接方法对特征稀少、灰度信息少和轮廓对称的钣金零件图像具有速度快、较好的准确性和抗干扰能力,能够满足工业应用的要求。 相似文献
22.
以烟草花叶病毒(TMV)为基本构建单元,研究了其在毛细管、载玻片-载玻片和柱透镜-硅片3种不同受限空间下的自组装行为。 结果表明,由于接触线有规律、重复性的粘滑运动,在3种受限体系下TMV均可组装得到高度规整的条带结构。 在毛细管内组装可得到垂直于管的长轴方向的有序条带。 随接触线深入管中心,条带跨距(l)与带宽(w)均逐渐增大。 在2个平行载玻片所构成的可控空间内所得到的条带以2条互相垂直的中心线为轴对称排列,并沿从载玻片边缘至轴向中心方向条带跨距和带宽梯度增大。 同样,利用柱透镜 硅片所组成的体系可得到类似平行线状条带,其跨距、带宽及条带高度沿从外部边缘至柱透镜/硅片接触中心方向逐渐减小。 在3种体系下,棒状TMV粒子均平行于接触线的方向取向。 TMV浓度对组装结构具有显著影响,随着浓度的增加,条带跨距和带宽均增加,直至条带结构消失形成连续膜。 相似文献
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By using a chiral cinchona alkaloid-squaramide catalyst, a series of both enantiomers of novel amino-pyrimidine derivatives can be obtained in an enantioselective three-component one-pot Mannich reaction with high yields and excellent enantioselectivities. In addition, these chiral derivatives were found to exhibit higher antiviral activities against tobacco mosaic virus (TMV) in vivo than the commercial agent ningnanmycin. In particular, chiral compounds (R)-4b and (R)-4e showed excellent antiviral activity against TMV at a concentration of 500?μg/mL, with a curative activity of 56.8% and 55.2%, respectively, a protection activity of 69.1% and 67.1%, respectively, and an inactivation activity of 91.5% and 94.3%, respectively. These values are superior to those of the agent ningnanmycin (which has curative, protective, and inactivation activities of 52.9%, 62.8%, and 90.4%, respectively). The antiviral mechanisms and enhanced antiviral activities of these chiral derivatives are interesting subjects for future investigation. 相似文献
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Yan-Jiao Wang Da-Gui Zhou Fang-Cheng He Ji-Xiang Chen Yong-Zhong Chen Xiu-Hai Gan De-Yu Hu Bao-An Song 《中国化学快报》2018,29(1):127-130
A series of novel chalcone derivatives containing purine group was synthesized and evaluated for their antiviral activities against cucumber mosaic virus and tobacco mosaic virus. Compound 3o exhibited remarkable antiviral activities and strong combining capacity to tobacco mosaic virus coat protein. 相似文献
27.
A sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) for the determination of cucumber mosaic virus (CMV) was proposed in this paper. The activity of labeled enzyme, horseradish peroxidase, was measured with electrochemical methods using o-phenylenediamine as substrate. The enzymatic reaction product is 2,3-diaminophenazine, which can be easily reduced on the dropping mercury electrode with improved sensitivity. Coupled with the plate trapped antigen indirect ELISA format using polyclonal rabbit antibody of CMV, the electrochemical detection was performed for CMV with the detection limit of 0.5 ng ml−1, which is ten times more sensitive than the colorimetric ELISA method. The conditions for enzymatic reaction and immunoassay were carefully optimized. 相似文献
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An intermediate expressing vector carrying the tobacco mosaic virus (TMV, Chinese common strain) coat protein (CP) gene was constructed by recombinant DNA techniques. The TMV-CP gene was transferred into the tobacco genome via Ti plasmid and a large number of regenerated plants, including both systemic and local lesion hosts for TMV, were obtained. Southern blot analysis revealed that 1-5 copies of the CP gene were integrated into the tobacco genome. RNA and protein analysis demonstrated that the TMV-CP gene was correctly expressed in the transgenic plants. The abundance of TMV-CP mRNA in total leaf RNA accounted for 0.005-0.01%, while the amount of coat proteins reached 0.05-0.2% of the total leaf soluble proteins. Virus challenge experiments showed that the symptom development of virus infection was markedly delayed and the replication as well as the spread of the virus was significantly inhibited in the transgenic plants expressing the TMV-CP gene. Three of these plants were completely protected afte 相似文献
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An approach based on depth-sensitive skew-angle x-ray diffraction (SAXRD) is presented for approximately evalu- ating the depth-dependent mosaic tilt and twist in wurtzite c-plane GaN epilayers. It is found that (103) plane and (101) plane, among the lattice planes not perpendicular to the sample surface, are the best choices to measure the depth profiles of tilt and twist for a GaN epilayer with a thickness of less than 2 μm according to the diffraction geometry of SAXRD. As an illustration, the depth-sensitive (103)/(101) ω-scans of a 1.4-μm GaN film grown by metal-organic chemical vapor deposition on sapphire substrate are measured and analyzed to show the feasibility of this approach. 相似文献