Thermal radiation impact on magneto-hydrodynamic heat transfer micropolar fluid flow over a vertical moving porous plate: A finite difference approach

For this research, an examination on the magnetohydrodynamic flow of a micropolar fluid across a moving vertical porous plate for the presence of thermal radiation is achieved. It is necessary to translate the partial differential equations regulating the flow, heat, & mass transfer into dimensionless form employing proper non-dimensional variables, which are then cracked numerically by utilizing the Finite difference approach. Graphs are used to represent numerical values of various flow profiles; however, tables are used to represent the simulated values of rate coefficients. The velocity rises when the value of Grashof number, dimensionless viscosity ratio is raised, and the opposite effect is seen when the value of magnetic parameter, micro-gyration factor is raised. The result in skin friction coefficient improves when the values of magnetic parameter, micro-gyration factor, Prandtl number, and radiation are raised higher.     

基于联合分步APIT定位算法的改进

本文研究了无线传感网络( Wireless Sensor Network,WSNs)的节点定位问题,并针对APIT由于锚节点在低密度环境下的节点误判和节点失效等问题给出了改进,在APICT定位算法的基础提出了联合分步定位算法UNION-APICT(Union Approximate Point-In-Circumcircle Test),该算法是结合连通性的测距技术,RSSI测距技术以及质心定位和APICT等技术,来联合解决对未知节点定位问题。通过仿真实验结果表明,改进后的UNION-APICT在APICT算法的基础之上平均定位误差减少了10%-25%,定位性能有了明显的提升;随着通信半径R和最大探测距离rmax的增加,定位误差也在逐渐减小,该算法较APIT和APICT定位算法在锚节点密度、节点覆盖率和定位精度上都有所提高。     

Sonocatalytic injury of cancer cells attached on the surface of a nickel–titanium dioxide alloy plate

The present study demonstrates ultrasound-induced cell injury using a nickel–titanium dioxide (Ni–TiO₂) alloy plate as a sonocatalyst and a cell culture surface. Ultrasound irradiation of cell-free Ni–TiO₂ alloy plates with 1 MHz ultrasound at 0.5 W/cm² for 30 s led to an increased generation of hydroxyl (OH) radicals compared to nickel–titanium (Ni–Ti) control alloy plates with and without ultrasound irradiation. When human breast cancer cells (MCF-7 cells) cultured on the Ni–TiO₂ alloy plates were irradiated with 1 MHz ultrasound at 0.5 W/cm² for 30 s and then incubated for 48 h, cell density on the alloy plate was reduced to approximately 50% of the controls on the Ni–Ti alloy plates with and without ultrasound irradiation. These results indicate the injury of MCF-7 cells following sonocatalytic OH radical generation by Ni–TiO₂. Further experiments demonstrated cell shrinkage and chromatin condensation after ultrasound irradiation of MCF-7 cells attached on the Ni–TiO₂ alloy plates, indicating induction of apoptosis.     

In situ X‐ray data collection and structure phasing of protein crystals at Structural Biology Center 19‐ID

A prototype of a 96‐well plate scanner for in situ data collection has been developed at the Structural Biology Center (SBC) beamline 19‐ID, located at the Advanced Photon Source, USA. The applicability of this instrument for protein crystal diffraction screening and data collection at ambient temperature has been demonstrated. Several different protein crystals, including selenium‐labeled, were used for data collection and successful SAD phasing. Without the common procedure of crystal handling and subsequent cryo‐cooling for data collection at T = 100 K, crystals in a crystallization buffer show remarkably low mosaicity (<0.1°) until deterioration by radiation damage occurs. Data presented here show that cryo‐cooling can cause some unexpected structural changes. Based on the results of this study, the integration of the plate scanner into the 19‐ID end‐station with automated controls is being prepared. With improvement of hardware and software, in situ data collection will become available for the SBC user program including remote access.     

高超声速平板边界层流动显示的试验研究

本文在高超声速脉冲式风洞内对基于纳米示踪的平面激光散射技术(nano-based planar laser scattering, NPLS)的应用进行了探索, 并在此基础上对平板边界层流动结构的精细测量进行了研究. 试验来流Ma=7.3, 总压4.8 MPa, 总温680 K. 通过时序的分析和调试, 对各分系统实现了高精度的同步控制; 定量的粒子注入及混合, 实现了粒子的均匀撒播, 对主流获得了均匀的显示效果; 对于边界层流动, 获得了精细的瞬态流动结构图像, 显示了层流到湍流的转捩过程, 并分析了其时空演化特性.     

Conservative numerical methods for the Full von Kármán plate equations

This article is concerned with the numerical solution of the full dynamical von Kármán plate equations for geometrically nonlinear (large‐amplitude) vibration in the simple case of a rectangular plate under periodic boundary conditions. This system is composed of three equations describing the time evolution of the transverse displacement field, as well as the two longitudinal displacements. Particular emphasis is put on developing a family of numerical schemes which, when losses are absent, are exactly energy conserving. The methodology thus extends previous work on the simple von Kármán system, for which longitudinal inertia effects are neglected, resulting in a set of two equations for the transverse displacement and an Airy stress function. Both the semidiscrete (in time) and fully discrete schemes are developed. From the numerical energy conservation property, it is possible to arrive at sufficient conditions for numerical stability, under strongly nonlinear conditions. Simulation results are presented, illustrating various features of plate vibration at high amplitudes, as well as the numerical energy conservation property, using both simple finite difference as well as Fourier spectral discretizations. © 2015 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 31: 1948–1970, 2015     

TNF-α、IL-6、IL-8在Modic I型改变腰椎终板与椎间盘髓核中的表达及关系研究

目的 探讨腰椎Modic I型改变的腰椎终板与椎间盘髓核中TNF-α、IL-6、IL-8 的相关性。方法 收集30 例因下腰痛入院手术治疗并行腰椎MRI 检查确认为腰椎Modic I型改变患者的术中病灶椎间盘标本,进行免疫组化检测,并观察椎间盘组织病理变化及炎症因子的表达,IL-6、IL-8 相对表达水平以相对表达量△Ct 或（2-△Ct×104）进行分析。结果 Modic I型终板中TNF-α表达水平（9.2±0.7）高于髓核（7.2±0.5）,差异有统计学意义（P＜0.05）;髓核中△Ct IL-6、△Ct IL-8 相对表达量（6.34±1.82、10.62±1.88）高于终板（4.07±1.86、5.92±1.31）,差异均有统计学意义（均P＜0.05）。结论 Modic I型终板中TNF-α、IL-6、IL-8的表达水平明显高于髓核,其引起下腰痛的原因主要为终板的炎症改变。     

使用曲面微通道板和感应电荷位置灵敏阳极的软X射线-极紫外光子计数成像探测器研究

本项目对我国空间探测的极紫外(EUV)波段大视场相机所需求的球面光子计数成像探测器的关键技术进行了研究。首先,建立了光阴极材料次级电子产出模型,利用该模型计算了软X射线-EUV波段常用的光电阴极材料—碱卤化物的次级电子产出,分析了微通道板(MCP)的次级电子产出。建立了测量MCP量子探测效率的装置,并推导出MCP量子探测效率的计算公式,测量了MCP在软X射线-EUV波段的量子效率以及MCP量子效率随掠入射角的变化。其次,建立了球面实芯微通道板的制备装置,利用高温热成型方法制备出曲率半径为150 mm球面MCP,利用光刻技术制备出有效直径为48 mm的楔条形感应电荷位置灵敏阳极,在此基础上集成了一套使用球面MCP和感应电荷位置灵敏阳极的两维光子计数成像探测器。再次,研制出包括快速前端模拟电路与后续数字电路的成像读出电路,编制了能矫正图像畸变的图像实时采集和处理软件。最后,建立了MCP探测器空间分辨率、图像线性的检测装置,对研制出的探测器性能进行了检测,检测结果表明:探测器的各项技术指标完全满足要求。     

Influence of the molding angle on tensile properties of FDM parts with orthogonal layering

Fused deposition molding (FDM) is one of the most widely used three‐dimensional (3D) printing technologies. This paper explores the influence of the forming angle on the tensile properties of FDM specimens. Orthogonal layering details were studied through experiments, theory, and finite element simulations. The stiffness and strength of the specimens were analyzed using the classical laminated plate theory and the Tsai–Wu failure criterion. The experimental process was simulated using finite element simulations. Results show that it is feasible to predict the stiffness and strength of FDM specimens using classical laminated plate theory and the Tsai–Wu failure criterion. A molding angle of 45° leads to specimens with maximized tensile properties. Numerical simulations show that changing the molding angle changes the internal stress and deformation fields inside samples, leading to FDM samples with different mechanical properties due to the orthogonal layers at different molding angles.     

Semisynthesis of Functional Glycosylphosphatidylinositol‐Anchored Proteins

Glypiation is a common posttranslational modification of eukaryotic proteins involving the attachment of a glycosylphosphatidylinositol (GPI) glycolipid. GPIs contain a conserved phosphoglycan that is modified in a cell‐ and tissue‐specific manner. GPI complexity suggests roles in biological processes and effects on the attached protein, but the difficulties to get homogeneous material have hindered studies. We disclose a one‐pot intein‐mediated ligation (OPL) to obtain GPI‐anchored proteins. The strategy enables the glypiation of folded and denatured proteins with a natural linkage to the glycolipid. Using the strategy, glypiated eGFP, Thy1, and the Plasmodium berghei protein MSP1₁₉ were prepared. Glypiation did not alter the structure of eGFP and MSP1₁₉ proteins in solution, but it induced a strong pro‐inflammatory response in vitro. The strategy provides access to glypiated proteins to elucidate the activity of this modification and for use as vaccine candidates against parasitic infections.