TGF-��-treated antigen presenting cells suppress collagen-induced arthritis through the promotion of Th2 responses

Collagen-induced arthritis (CIA) is mediated by self-reactive CD4⁺ T cells that produce inflammatory cytokines. TGF-β₂-treated tolerogenic antigen-presenting cells (Tol-APCs) are known to induce tolerance in various autoimmune diseases. In this study, we investigated whether collagen-specific Tol-APCs could induce suppression of CIA. We observed that Tol-APCs could suppress the development and severity of CIA and delay the onset of CIA. Treatment of Tol-APCs reduced the number of IFN-γ- and IL-17-producing CD4⁺ T cells and increased IL-4- and IL-5-producing CD4⁺ T cells upon collagen antigen stimulation in vitro. The suppression of CIA conferred by Tol-APCs correlated with their ability to selectively induce IL-10 production. We also observed that treatment of Tol-APCs inhibited not only cellular immune responses but also humoral immune responses in the process of CIA. Our results suggest that in vitro-generated Tol-APCs have potential therapeutic value for the treatment of rheumatoid arthritis as well as other autoimmune diseases.     

Simultaneous Separation and Determination of Benzoic Acid Compounds in the Plant Medicine by High Performance Capillary Electrophoresis

A simple and inexpensive high performance capillary electrophoresis (HPCE) was applied to separate five benzoic acid compounds simultaneously. The investigation was carried out by micellar electrokinetic capillary chromatography (MECC). To avoid a time‐consuming and tedious procedure, orthogonal experimental design OA₉ (3⁴) for separation experiments was applied to find the optimal conditions in terms of the resolution and analytical time. The best conditions for separation were obtained using a 20 mM borax and 30 mM sodium dodecyl sulfate (SDS) buffer (pH 9.8) containing 2 mM β‐CD and 4% methanol (v/v). Online UV detection was performed at 250 nm. A voltage of 16 kV was applied and the temperature was controlled at 25 °C. Injection was performed for 5 s. The method was validated for the quantification of benzoic acid, salicylic acid and ortho‐aminobenzoic acid in Radix Isatidis, a traditional plant medicine with removal of endotoxin. The separation and determination were satisfactory and quick.     

地方高校省级物理示范实验室建设的探索与实践

提出了示范实验室建设的思路与目标,阐述分层次模块化的实验教学体系,介绍了多样化的实验教学手段。经过3年的探索与实践,示范实验室建设成效显著、特色明显,取得了较为显著的成绩,有一定示范性和辐射作用。     

纳秒脉冲下聚合物电树枝引发实验方法

 借鉴直流、交流的研究经验,比较了纳秒脉冲条件下几种不同的电树枝老化实验方法。对单针-板电极和多针-板电极在纳秒脉冲下实验结果的一致性进行考察,结果表明,多针-板电极系统可以在提高实验效率的同时保证结果的准确性。采用步进法和累加法进行了不同频率下聚苯乙烯电树枝引发实验,结果表明:两种方法得到的纳秒脉冲下聚苯乙烯电树枝引发电压-频率特性基本一致,在50~500 Hz范围内,引发电压随频率的升高而降低;在500~800 Hz范围内,引发电压随频率的升高而增加。最后讨论了对于不同脉冲功率装置中绝缘材料老化试验设计的方法。     

Optical and atomic force microscopic study on step bunching in BaB2O4 crystal growth

The formation of macrostep during high-temperature phase of barium meta-borate (α-BaB 2 O 4) single crystal growth has been investigated by both optical in-situ observation system and atomic force microscopy (AFM).The insitu observation results demonstrate that the critical linear size of growing facet exceeding the size that the macrostep generates is significantly anisotropic.The critical linear sizes are around 280 μm and 620 μm for {1010} and {1010} planes,respectively.AFM study illustrates that macrostep train with a height of 150 nm～200 nm is one typical morphological feature of the as-grown crystal surface.The riser of each macrostep consists of several straight and parallel sub-steps,indicating the occurrence of step bunching.Additionally,triangular sub-steps with heights of several nanometers on the treads of the macrosteps are found to be another typical feature of surface morphology,which implies a microscopically competitive bunching of sub-steps between various crystallographic orientations.     

Co离子注入TiO2薄膜的显微结构和磁性研究

利用直流磁控溅射技术在玻璃衬底上沉积了TiO₂薄膜,并对其进行了Co离子注入,最后在真空中500 ℃退火50 min,得到系列薄膜样品. 利用剥离-分散方法制备了薄膜的透射电镜样品,并用扫描电镜(SEM)、X射线能量散射谱(EDX)和高分辨透射电镜(HRTEM)对样品做了近似原位观察,研究了薄膜样品中不同Co离子注入深度的成分分布和显微结构. 结果表明,薄膜呈锐钛矿结构,Co元素主要分布在薄膜表层,Co离子的注入使TiO₂薄膜的晶粒被部分破坏,并形成CoO,而5 关键词： 2薄膜')" href="#">Co注入TiO₂薄膜 电镜原位观察 室温铁磁性     

透射光栅的实验标定和衍射效率的理论模拟

透射光栅广泛应用于软X射线能谱测量.为了获得用于惯性约束聚变研究的透射光栅的各级衍射效率及其他参数,在北京同步辐射源上200—1600 eV能量范围内对其进行了标定,获得了透射光栅衍射效率的实验结果.扩展了透射光栅衍射效率的计算方法,提出了7边准梯形截面衍射效率计算模型.分析拟合了实验数据,理论结果与实验结果很好符合.得到了7边准梯形的透射光栅栅线截面结构. 关键词： 透射光栅 衍射效率 实验标定 光栅模型     

微尺度水平单管内流动沸腾特性实验研究

对非共沸混合工质R134a/R32(75/25)在水平微尺度管道内的流动沸腾换热实验结果进行了分析和讨论,以探究微细通道内流动沸腾换热的主导机制。对影响其换热的多种因素(热流密度、质量流量和质量干度)进行了分析,实验得出,当质量干度较低时,热流密度和质量流量共同控制着微尺度管内的换热方式,当热流密度的影响占主导地位时,管道内的换热以核态沸腾为主;当质量流量的影响占主导地位时,管道内的换热以强制对流为主。     

车辆天窗气动噪声的数值分析与实验研究

本文从汽车天窗气动噪声的机理入手,利用与实车几何尺寸为1:5的简化模型进行了空腔绕流的数值计算,分析了其流场结构及气动噪声产生的原因.在低速静音风洞中进行了不同流速下的流场和声场实验研究,研究了天窗不同位置的速度剪切层变化,以及不同流速下的声压级变化,发现了除了特征频率下的风振噪声,还存在较大频率范围的气动噪声,其随着...