The Use of Microporous Divinyl Benzene Copolymer for Yeast Cell Immobilization and Ethanol Production in Packed-Bed Reactor

Microporous divinyl benzene copolymer (MDBP) was used for the first time as immobilization material for Saccharomyces cerevisiae ATCC 26602 cells in a bed reactor and ethanol production from glucose was studied as a model system. A very homogenous thick layer of yeast cells were seen from the scanning electron micrographs on the outer walls of biopolymer. The dried weight of the cells was found to be approximately 2 g per gram of cell supporting material. Hydrophobic nature of polymer is an important factor increasing cell adhesion on polymer pieces. The dynamic flow conditions through the biomaterial due to its microporous architecture prevented exopolysaccharide matrix formation around cells and continuous washing out of toxic metabolites and dead and degraded cells from the reactor provided less diffusional limitations and dynamic living environment to the cells. In order to see the ethanol production performance of immobilized yeast cells, a large initial concentration range of glucose between 6.7 and 300 g/l was studied at 1 ml/min in continuous packed-bed reactor. The inhibition effect of glucose with increasing initial concentration was observed at above 150 g/l, a relatively high substrate concentration. The continuous fluid flow around the microenvironment of the attached cells and mass transferring ability of cell immobilized on MDBP can help in decreasing the inhibition effect of ethanol accumulation and high substrate concentration in the vicinity of the cells.     

Styryl Dyes as Two-Photon Excited Fluorescent Probes for DNA Detection and Two-Photon Laser Scanning Fluorescence Microscopy of Living Cells

Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2–3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids- containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.     

Iterated egalitarian compromise solution to bargaining problems and midpoint domination

We introduce a new solution for two-person bargaining problems: the iterated egalitarian compromise solution. It is defined by using two prominent bargaining solutions, the egalitarian solution (Kalai, 1977) and the equal-loss solution (Chun, 1988), in an iterative fashion. While neither of these two solutions satisfy midpoint domination – an appealing normative property – we show that the iterated egalitarian compromise solution does so.     

Synthesis,Characterization and Bactericidal Properties of Poly(N-vinyl-2-pyrrolidone-co-Maleic anhydride-co-N-Isopropyl acrylamide)

Summary: Radical-initiated terpolymerization of maleic anhydride (MA), N-vinyl-2-pyrrolidone (NVP) with N-isopyropyl acrylamide (NIPA) has been prepared as a way to obtain new water-soluble polymers. Structure, composition and thermal behaviour of synthesized terpolymers were determined by FTIR, UV-vis, ¹H NMR spectroscopy, elemental analysis (N content), differential scanning calorimetry, thermogravimetric and differential thermal analysis, gel permeation chromatography analysis (GPC), scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD). The terpolymer composition-structure-property relationship indicates that the semicrystalline structure of terpolymers with different reaction times, degrees of crystallinity, and thermal behaviour depends on the content of carboxyl and amide-containing monomer linkage. The antimicrobial activities the terpolymers were evaluated against pathogen bacteria: Staphylococcus aureus, Salmonella enteridis, Streptococcucs faecalis, Eschericia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The antimicrobial activity was explored by the well-diffusion technique. All the studied polymers, containing biologically active moieties in the form of ionized cyclic amide and carboxylic acid groups, were more effective against Gram-positive than Gram-negative bacteria. The bactericidal effect of terpolymers against Gram negative bacteria increased with the increasing reaction times.     

Fluorescence of Styryl Dyes-DNA Complexes Induced by Single- and Two-Photon Excitation

The series of novel monomer and homodimer styryl dyes based on (p-dimethylaminostyryl) benzothiazolium residues were synthesized and studied as possible fluorescent probes for nucleic acids detection. Spectral-luminescent and spectral-photometric properties of obtained dyes in the unbound state and in DNA presence were studied. Fluorescence emission induced by two-photon excitation of dye-DNA complexes in aqueous buffer solution was registered. Two-photon absorption cross section values of the studied dyes in DNA presence were evaluated.     

Synthesis of new polyhedral oligomeric silsesquioxane derivatives as some possible antimicrobial agents

1,3-Dipolar cycloaddition reactions were studied to synthesize Polyhedral oligomeric silsesquioxane (POSS)-based norbornyl imide derivatives containing izoxazoline groups in good yields. And also 1,3-dipolar cycloaddition reactions of azomethine ylides with POSS-based norbornene dipolarophiles for a synthesis of the novel POSS-based norbornane-fused spiro-1,3-indandionolylpyrrolidines are reported. All newly synthesized POSS compounds were structurally characterized by FTIR, ¹H, ¹³C NMR, HRMS and GC/MS analyses.     

Producing of qualified oil and carbon black from waste tyres and pet bottles in a newly designed pyrolysis reactor

Journal of Thermal Analysis and Calorimetry - Waste tyre and waste polyethylene terephthalate (PET) bottles are major environmental problems. In this study, by using newly designed simple and...     

Synthesis,photophysical and antioxidant properties of pyrrolo[3,2-c]carbazole and dipyrrolo[3,2-c:2′,3′-g]carbazole compounds

Research on Chemical Intermediates - The synthesis of (6-ethyl-1,6-dihydropyrrolo[3,2-c]carbazol-2-yl)methanol 5 and...     

An Integrated Inventory-Routing System for Multi-item Joint Replenishment with Limited Vehicle Capacity

In this paper, we develop a mathematical programming approach for coordinating inventory and transportation decisions in an inbound commodity collection system. In particular, we consider a system that consists of a set of geographically dispersed suppliers that manufacture one or more non-identical items, and a central warehouse that stocks these items. The warehouse faces a constant and deterministic demand for the items from outside retailers. The items are collected by a fleet of vehicles that are dispatched from the central warehouse. The vehicles are capacitated, and must also satisfy a frequency constraint. Adopting a policy in which each vehicle always collects the same set of items, we formulate the inventory-routing problem of minimizing the long-run average inventory and transportation costs as a set partitioning problem. We employ a column generation approach to determine a lower bound on the total costs, and develop a branch-and-price algorithm that finds the optimal assignment of items to vehicles. We also propose greedy constructive heuristics, and develop a very large-scale neighborhood (VLSN) search algorithm to find near-optimal solutions for the problem. Computational tests are performed on a set of randomly generated problem instances.The work of this author was supported by a scholarship of the Faculty of Engineering of Ubonratchathani University, Ubonratchathani, Thailand., The work of this author was supported in part by the National Science Foundation under Grant No. DMI-0085682.     

Model for the transition to the radiatively improved mode in a tokamak

A model for the transition to the radiatively improved (RI) mode triggered in tokamaks by seeding of impurities is proposed. This model takes into account that with increasing plasma effective charge the growth rate of the toroidal ion temperature gradient (ITG) instability, considered nowadays as the dominant source of anomalous energy losses in low-confinement (L) mode, decreases. As a result the plasma density profile peaks due to an inward convection generated by trapped electron turbulence. This completely quenches ITG induced transport and a bifurcation to the RI mode occurs. Conditions necessary for the L-RI transition are investigated.