原子量测定发展史与原子量基准的国际化历程

从古代思辨的原子论到近代科学的原子-分子论,原子量的测定是其从定性到定量发展的关键。原子量为化学家们研究物质提供了新的思路。原子量内涵的相对性,决定了原子量基准的重要性。原子量最终取代了测定与传播都早于它的当量,并走向了国际化,这个过程不仅可让我们认识到科学发展的曲折艰辛,也让人们理解了科学交流的重要性。     

解析电喷雾质谱法对感冒药中对乙酰氨基酚的快速检测

基于常压质谱的直接、快速、无需样品预处理检测的优势,该文拓展了其在药物质检领域的应用。采用自行搭建的解析电喷雾(DESI)装置,对常用感冒药对乙酰氨基酚片进行快速质谱检测,无需样品预处理,直接获得药片中有效成分的分子结构信息。为克服基质差异对定量分析的影响,以淀粉为基质构建对乙酰氨基酚模拟药片,在优化的基础上进行定量实验。针对实际药片中高浓度对乙酰氨基酚检测的需要,研究了其在较高浓度范围的定量关系,结果显示,方法对0.35~4.52 mg/mm^2范围内的对乙酰氨基酚具有较好的线性关系(r^2=0.9982),定量下限为1.903 ng/mm^2,检出限为0.237 ng/mm^2,加标回收率为102%~114%。对3种市售的对乙酰氨基酚片进行直接检测,与厂家提供的标准数据相比,相对标准偏差(RSD)为7.2%~12%,表明方法具有较好准确度。该工作很好地证明了DESI-MS在药品快检中的优势,从而为药品质检提供了潜在的高效、可靠的检测手段。     

硫代核苷类似物合成研究进展

4′-硫代核苷类似物是核糖环中的氧原子被硫原子取代的核苷类似物。许多硫代核苷具有良好的抗病毒和抗肿瘤活性。4′-硫代核苷具有更稳定的糖基键和针对各种病毒或细胞酶的代谢稳定性的提升等固有优点。因此,对硫代核苷类似物的设计与合成,筛选出安全有效的抗病毒试剂应受到更多药物化学家们的关注,本文综述了近年来硫代核苷的合成研究进展。     

A Germanate Compound Constructed from Dissymmetric Ge7 Chains and Metal Complexes

Abstract. A novel germanate compound, |[Ni(dien)₂]₃(H₂O)₃|[Ge₇O₁₃F₅]₂(designated JU‐85, dien = diethylenetriamine), was solvothermally synthesized. The structure of JU‐85 was determined by single‐crystal X‐ray diffraction and further characterized by powder X‐ray diffraction, inductively coupled plasma, infrared spectroscopy, elemental analysis, and thermogravimetric analysis. JU‐85 has dissymmetric chains constructed from diagonally linked Ge₇ building units and various Ni(dien)₂²⁺ complexes formed in situ during the synthesis. Compared with its structural analogue, FJ‐6, JU‐85 contains less complex cations and different host‐guest assembly. Besides the diagonal linkage in JU‐85, other dissymmetric linkages of Ge₇ building units were enumerated, which could be used as the stereogenic centers for the design of novel chiral germanate compounds.     

Shears mechanism in two-dimensional cranking relativistic mean field approach

Using the new two-dimensional cranking relativistic mean field (RMF) approach, the shears mechanism of magnetic rotation based on configuration πh121/2νh-211/2 in 142Gd is microscopically and self-consistently examined by investigating the aligning angular momenta of the valence nucleons.     

Beam dynamics studies on BEPC-Ⅱstorage rings at the commissioning stage

During the 1st and 2nd stages of the commissioning of the upgrade project of the Beijing Electron Positron Collider(BEPC Ⅱ),which started on Nov.12,2006 and Oct.24,2007,respectively,we got the luminosity one tenth of its design value,provided beams to synchrotron radiation users for about 4 months,and studied beam dynamics as well.In this paper,some beam dynamics studies on the storage rings and their preliminary results are given.     

毛细管探针法测定液体表面张力系数的进一步研究

采用较大直径的毛细管对探针法测量液体表面张力系数做了进一步研究.文中引用了Prokhorov提出的改进的瑞利公式,介绍了表面张力系数α的各相关公式的适用范围.实验中利用毛细管探针法测量了几个不同温度下蒸馏水在一直径为5.744.nm的毛细管中的上升高度,用Prokhorov公式计算α.实验测得的蒸馏水的表面张力系数与公认值的百分误差约小于0.13%.最后对实验结果的误差原因进行了分析.     

Purification and Characterization of Protein Tyrosine Phosphatase MEG1 and Preparation of Anti-PTPMEG1 Antibody

<正>PTPMEG1 is an intracellular protein tyrosine phosphatase(PTP),which contains FERM and PDZ domains. This study focuses our attention on the expression,purification and characterization of catalytic domain of PTPMEG1(△MEG1) and preparation of its polyclonal antibody.A cDNA fragment encoding△MEG1 protein(amino acid residues 643—926) was amplified by PCR and then cloned into the pT7-7 vector.Both soluble and insoluble recombinant△MEG1 proteins were observed after induction by IPTG.Soluble△MEG1 was purified via two chromatographic steps,and the purified enzyme was characterized.With para-nitrophenylphosphate(pNPP) as a substrate,△MEG1 exhibited typical enzymatic characteristics of classic PTPs and classical Michaelis-Menten kinetics.Insoluble△MEG1,which was mainly distributed in the inclusion body of E.coli cells extracts,was purified by preparative electrophoresis gel for the preparation of the polyclonal antibody.A rabbit was immunized with△MEG1 purified by preparative electrophoresis to generate anti-△MEG1 antibody.Anti-serum was collected on 28th day after initial injection and purified via affinity chromatography.The purified polyconal antibody displayed a satisfactory titer and sensitivity.     

管内插新型自旋转子的流阻及抑垢特性

提出了一种新型自旋转子,并通过数值及实验方法研究了其流动阻力及抑垢特性.实验结果表明,在流速为0.76 m/s时转子转速仍可达100r/min,内插该转子的流动阻力比光管增加2倍左右,正反旋向转子组(每组4个转子)相问布置可防止沿流向转子转速递减,使转子转速基本恒定.数值分析表明,通过减小转子的旋转轴套外径并加前端导流...     

茜素红S-铕(Ⅲ)-米诺环素体系的共振瑞利散射光谱研究及应用

在pH=4.4的NaAc-HAc缓冲溶液中,茜素红S-铕(Ⅲ)与米诺环素形成三元配合物,导致共振瑞利散射(RRS)增强,据此建立了一种测定米诺环素的RRS分析法。在λ=370nm处,米诺环素浓度在0.073～5.493μg/mL范围内与体系RRS强度呈良好的线性关系,检出限为0.021μg/mL。该方法灵敏度高,简单、快速,具有良好的选择性和重复性,对尿样进行加标回收及用于片剂、胶囊中米诺环素的测定,结果满意。