定量薄层色谱法的现状与展望

今天,薄层色谱法是指仪器化高效薄层色谱法。它已成为公认的、应用广泛的通用分析方法。1989年创办了国际性薄层色谱杂志(J.Planar Chromatogr.—Modern TLC)。在涉及分离分析内容的出版物中,几乎都能找到有关薄层色谱法的章节     

药物分析

本文对国内药物分析在１９９２－１９９３年间的主要进展作了评述。内容集中于分光光度法、高效液相色谱法、气相色谱法、薄层色谱法、电化学分析法及其它分析方法等。共引用文献９３５篇。     

气相色谱法快速测定食品中山梨酸和苯甲酸

苯甲酸、山梨酸作为食品添加剂广泛应用于食品、饮料等。其使用限量各国对于不同的食品有不同的要求。有关食品中苯甲酸、山梨酸的测定方法报道较多,有分光光度法、液相色谱法、薄层色谱法及气相色谱法。由于气相色谱法较为普及且应用较为方便,通常都采用气相色谱法进行日常分析。我们在应     

离子色谱法在中药化学成分分析中的应用进展

离子色谱作为一种新型高效液相色谱技术, 最初主要应用于测定样品中的阴阳离子含量, 如今发展为应用于有机酸、生物胺、糖类等化合物的组成及含量测定. 离子色谱法具有操作简便、灵敏度高以及选择性好等优点, 因此, 目前离子色谱法已在能源、环境、地质、食品、药物等领域得到了广泛应用. 总结了近年来离子色谱在中药金属离子、无机阴离子、有机酸、糖类成分的研究进展, 为离子色谱法分析中药化学成分提供了参考.     

翻译后修饰蛋白质组学分离方法的研究进展

蛋白质翻译后修饰（PTMs）是调节细胞内生理活动的重要途径。该文总结了近年来PTMs蛋白质组学相关的分离方法,包括反相（RP）色谱法、离子交换（IEX）色谱法、亲水相互作用色谱（HILIC）法、多孔石墨化碳（PGC）色谱法、毛细管电泳（CE）法及分子筛色谱（SEC）法等。这些新方法为磷酸化、乙酰化、糖基化等PTM肽段或蛋白质的鉴定提供了更高的分离度和灵敏度。此外,该文也介绍了蛋白质领域其他重要分离方法的研究进展,这些方法可能被进一步应用于PTMs蛋白质组学的研究中。     

衍生化反相高效液相色谱测定葡萄酒中有机酸的研究

葡萄酒中有近百种有机酸,其中主要是乳酸、乙酸、酒石酸、苹果酸、柠檬酸和琥珀酸,它们的含量占葡萄酒中有机酸总量的95％以上。这些酸的含量决定着葡萄酒的口感、品质和稳定性等特性。葡萄酒中有机酸的主要测定方法有酸碱滴定法、纸色谱法、薄层色谱法“和气相色谱法等。近年来,离子色谱法和直接高效液相色谱法得到重视,但由于检测方法的限制和葡萄酒中其它物质的干扰,使这两种方法的检测灵敏度及定量结果受到影响。     

高效液相色谱测定盐酸金霉素及其杂质

金霉素属于四环素类抗生素,在金霉素的生产和贮存期间产生一些四环素类的副产物,有的有一定药效,有的是有害物质。目前我国对金霉素的检验采用微生物效价测定法和薄层色谱法。国外近年内有用高效液相色谱法,但至今未见公开报道。经过一段时间的摸索,我们建立了高效液相色谱法测定金霉素及其杂质的方法,并发现了以前未知的杂质。     

金属离子对部分阴离子色谱测定的干扰及消除

本文对 F~-、柠檬酸的离子色谱法测定中 Al~(3+)、Fe~(3+)的干扰,和三乙醇胺、柠檬酸、磺基水杨酸对离子色谱法测 F~-时 Al~(3+)的干扰掩蔽及 EDTA 和磺基水杨酸对离子色谱法测柠檬酸时 Fe~(3+)、Al~(3+)干扰的掩蔽进行了讨论。     

气相色谱法测定汽油烃类组成分析技术的应用现状与发展

对气相色谱法测定汽油馏分烃类组成分析技术的应用现状和发展进行了系统评述。分别对高分辨毛细管气相色谱法、多维气相色谱法和溴加成结合气相色谱-原子发射光谱检测法测定汽油烃类组成的特点和存在的问题进行了讨论,并提出了一些解决方案。     

银杏磷脂复合物复合率的测定

为制定银杏磷脂复合物的制备工艺的评价标准．以高效液相色谱法测定复合物中黄酮醇苷的含量,计算复合率,以此为评估标准,用正交设计试验优化了制备工艺．确定了高效液相色谱法测定复合物复合率的方法,并对复合物中的银杏内酯进行了鉴别。     

色谱专家系统的应用和发展

随着科学技术的发展,色谱专家系统作为一种人工智能方法亦在不断发展,并在实际工作中发挥着越来越重要的作用。该文综述了作者的研究小组在色谱专家系统研究方面的进展,重点介绍从研究气相色谱专家系统开始,到应用色谱专家系统的思想,运用气相色谱、液相色谱和毛细管电泳新技术解决石化、环境、疾病诊断、基因分析和药物分析等领域实际问题的工作。引用相关文献64篇。     

A review of liquid chromatography in environmental pesticide analysis

Summary The use of liquid chromatography in environental pesticide analysis is reviewed. In addition to classical UV and electrochemical detectors, detection systems such as mass spectromytry and gas chromatographytype detectors are discussed. The applicability of supercritical fluid chromatography to pesticide analysis is also reviewed.Presented at the 18th International Symposium on Environmental Analytical Chemistry, Barcelona 5–8 September, 1988.     

Advances in the thin-layer chromatographic forensic analysis of inks

Advances in the use of thin layer and high-performance thin-layer chromatography for analysis of inks and ink writings in forensic investigations are reviewed. Experimental materials and techniques such as sample preparation, layers, sample application, development, detection, documentation, and interpretation of results are described and selected applications for several ink types are given. Some additional analytical techniques that complement thin-layer chromatography are also mentioned. Citations to important literature, from 2008 to 2016, are included.     

氢气的分析方法研究

介绍了低纯度氢气和高纯度氢气的不同分析检测方法。对于纯度小于99.99%的氢气,其分析方法主要有爆炸法、吸收法、膜分离法和气相色谱法;对于纯度大于99.99%的氢气,其分析方法主要有变温浓缩的热导池气相色谱法、配有光电离子化检测器(DID)的气相色谱法和色谱-质谱联用法。     

食品中有机酸的高效液相色谱分析法

对离子交换色谱法、离子排斥色谱法和反相高效液相色谱法分析食品中有机酸的特色和近几年的研究与应用状况作一综述。     

Analysis of enhanced oil recovery formulations

Summary Methods for the determination of low levels of surfactants in produced oil field brines have been developed. Alkylbenzene sulfonates (ABS) and alkylether sulfates can be quantified at very low levels by high-performance liquid chromatography (HPLC) and mobile-phase ion chromatography (MPIC), respectively. These methods proved to be rapid, selective, and gave results in excellent agreement with traditional low-phase titration techniques. Although this paper records the use of these methods for analysis of oil field produced fluids, they should be easily adaptable to other situations wherein the analysis of these two classes of surfactants is required.     

因子分析在色谱研究中的应用

本文综述了近几年来化学因子分析法在色谱研究中的进展。文章略论了因子分析与色谱的关系，对因子分析应用于色谱理论研究及色谱组分分析作了较多的评述，并展望了因子分析在色谱中的应用前景。     

Determination of chlorogenic acid,polyphenols and antioxidants in green coffee by thin‐layer chromatography,effect‐directed analysis and dot blot – comparison to HPLC and spectrophotometry methods

The great prevalence of thin‐layer chromatography over high‐performance liquid chromatography is connected with the possibility of analyzing many samples in parallel. Therefore, the method is often used in screening and/or effect directed analysis to compare composition and chemical/biological properties of many samples in one run. It was already proved, that high performance thin‐layer chromatography, in many cases, can replace high‐performance liquid chromatography for quantitative analysis. The main aim of the paper is to show that simple thin‐layer chromatography can also be used as a quantitative or at least as a semi‐quantitative method, even when it concerns effect directed analysis e.g. direct bioautography. Chlorogenic acid content was measured in four methanol extracts of various green coffees and in one extract of black coffee using thin‐layer chromatography with ultraviolet detection and thin‐layer chromatography with effect directed detection. High‐performance liquid chromatography was used as a reference method. Additionally, total contents of polyphenols and antioxidants were estimated using thin‐layer chromatography or dot‐blot on chromatography plates. These results were compared to spectrophotometric methods. It was proved that thin‐layer chromatography can be used as a quantitative (using densitometry) or semi‐quantitative method (using other detection methods including effect directed detection) as well as for estimating total antioxidants or polyphenols content.     

Characteristics and enantiomeric analysis of chiral pyrethroids

Pyrethroids are synthetic pesticides that originated from the modification of natural pyrethrins to improve their biological activity and stability. They are a family of chiral pesticides with a large number of stereoisomers. Enantiomers of synthetic pyretroids present different insecticidal activity, toxicity against aquatic invertebrates and persistence in the environment so the development of rapid and sensitive chiral methods for the determination of different enantiomers is necessary. Several techniques have been employed for this purpose including gas chromatography, high performance liquid chromatography or more recently capillary electrophoresis and sub or supercritical fluid chromatography. A general view on the different chiral separation methods applied to the analysis of pyrethroids and the most important information about these pesticides is provided in this review.     

Gas chromatography vacuum ultraviolet spectroscopy: A review

Accelerated technological progress and increased complexity of interrogated matrices imposes a demand for fast, powerful, and resolutive analysis techniques. Gas chromatography has been for a long time a ‘go‐to’ technique for the analysis of mixtures of volatile and semi‐volatile compounds. Coupling of the several dimensions of gas chromatography separation has allowed to access a realm of improved separations in the terms of increased separation power and detection sensitivity. Especially comprehensive separations offer an insight into detailed sample composition for complex samples. Combining these advanced separation techniques with an informative detection system such as vacuum ultraviolet spectroscopy is therefore of great interest. Almost all molecules absorb the vacuum ultraviolet radiation and have distinct spectral features with compound classes exhibiting spectral signature similarities. Spectral information can be ‘filtered’ to extract the response in the most informative spectral ranges. Developed algorithms allow spectral mixture estimation of coeluting species. Vacuum ultraviolet detector follows Beer–Lambert law, with the possibility of calibrationless quantitation. The purpose of this article is to provide an overview of the features and specificities of gas chromatography–vacuum ultraviolet spectroscopy coupling which has gained interest since the recent introduction of a commercial vacuum ultraviolet detector. Potentials and limitations, relevant theoretical considerations, recent advances and applications are explored.