以meso-四(N-甲基-3-吡啶基)卟啉荧光光度测定痕量锌的研究

研究了以meso-四(N-甲基-3-吡啶基)卟啉测定锌痕量锌的荧光光度法, 其灵敏度较其它水溶性卟啉衍生物测定锌的分光光度法要高, 检出量为1ppb, 线性范围为0-1.0μg/25mL. 方法已用于测定自来水和头发中的微量锌, 结果满意.     

新荧光试剂5-(4''-卤代-2''-羧基苯偶氮)绕丹宁的合成及分析化学特性研究

合成了三种新荧光试剂,5—(4’—氯—2’—羧基苯偶氮)绕丹宁(CRACP,2)、5—(4’—溴—2’—羧基苯偶氮)绕丹宁(BRACP,3)及5—(4’—碘—2’—羧基苯偶氮)绕丹宁(IRACP,4).研究了它们的吸收光谱及荧光性质,探讨了分子结构与荧光性能的关系.建立了铜(Ⅱ)的荧光分析方法.在pH5.3时,CRACP与铜(Ⅱ)形成荧光螫合物,λ_(ex)/λ_(em)=310nm/408nm,检测限为5×10 ~(-4)μg/g.     

新荧光试剂3—对甲苯基—5—（2‘—羧基苯偶氮）绕丹宁的合成及荧光光度…

本文报道了新荧光试剂３－对甲苯基－５－（２＇－羧基苯偶氮）绕丹宁的合成。用元素分析、红外光谱、核磁共振氢谱和质谱确证了其结构，研究了其荧光性质。在ＰＨ５．６时，该试剂与Ｃｕ＾２＋形成稳定的荧光螯合物，且在λｅｘ／λｅｍ＝３０５ｎｍ／４０５ｎｍ产生强烈荧光。     

新荧光试剂5-(4'-卤代-2'-羧基苯偶氮)绕丹宁的合成及分析化学特性研究

合成了三种新荧光试剂,5-(4'-氯-2'-羧基苯偶氮)绕丹宁(CRACP.2).5-(4'-溴-2'-羧基苯偶氮)绕丹宁(BRACP,3)及5-(4'-碘-2'-羧基苯偶氮)绕丹宁(IRACP,4).研究了它们的吸收光谱及荧光性质.探讨了分子结构与荧光性能的关系.建立了铜(Ⅱ)的荧光分析方法.在pH5.3时.CRACP与铜( Ⅱ) 形成荧光螯合物, λ~ e~ x/ λ~e~m=310nm/408nm.检测限为5×10^-^4μg/g.     

1,3,5,7-四甲基-2,6-二乙酯基-8-(3’,4’-二氨苯基)-二氟化硼-二吡咯甲烷荧光光度法灵敏测定血液中亚硝基硫醇

S-亚硝基硫醇在生物体内具有传递信号、改变蛋白质的结构和功能、阻止有害物质对细胞的伤害等重要的生理作用.建立了一种灵敏、简单的应用荧光探针1,3,5,7-四甲基-2,6-二乙酯基-8-(3',4'-二氨苯基)-二氟化硼-二吡咯甲烷标记并测定S-亚硝基硫醇的荧光光度法:在1.0×10-4mol/L Hg2+存在下,荧光探针与S-亚硝基硫醇在30℃反应15min生成衍生物,在激发和发射波长分别为500nm和510nm处测定其荧光强度.荧光强度和S-亚硝基硫醇的浓度在(8.0～800.0)×10-9mol/L范围内成良好的线性关系.当信噪比等于3时,方法的检出限为6.0×10-10mol/L.该方法对健康人与心血管疾病患者血液中S-亚硝基硫醇的测定的加标回收率为97.46%～103.10%.     

荧光光度法测定头孢菌素类抗生素含量的研究进展

对近40年来利用荧光光度法测定头孢菌素类抗生素的研究进展进行了综述,包括利用药物酸性或碱性降解产物的荧光光度法、利用药物与金属离子的氧化还原和络合作用的荧光光度法和利用药物与有机荧光试剂反应的荧光光度法(引用文献30篇)。     

荧光光度法测定血清中碱性磷酸酶

合成了一种新的底物水杨酸磷酸酯 (SP),用于荧光光度法测定血清中碱性磷酸酶(ALP)活力.在37.0 ℃的Tris-HCl缓冲液(pH 9.0)条件下, 碱性磷酸酶作用于荧光底物SP,水解生成强荧光产物水杨酸(SA),生成的SA的量与参与反应的ALP 的活力成正比.据此建立了荧光光度法测定血清中碱性磷酸酶活力的新方法.测定碱性磷酸酶的线性范围是0.03～6.00 U/L,检测限为7.04 mU/L.本方法适用于血清中碱性磷酸酶的测定.测定结果与临床上常用的以对硝基苯磷酸酯为底物的分光光度法相比,无显著性差异.     

荧光光度法测定稀土元素研究进展

文章综述了近20年来国内外有关稀土元素荧光光度法的研究进展.主要从测定体系、测定波长、检出限、线性范围等方面对铈、铕、钐、镝、铽、镧、钇等稀土元素进行研究.最后对一些新的荧光技术如激光诱导时间分辨光谱、时间分辨荧光免疫分析等进行了简单讨论.文章引用文献69篇.     

秩消因子分析—荧光光度测定某些食物中的营养元素硒

本文应用秩消因子分析－荧光光度法测定了食物中营养元素硒的含量，与文献〔１〕报道的ＤＡＮ－荧光光度法测定结果比较，具有良好的统计学一致性，从而为简化实验操作，改善痕量硒测定的准确性和灵敏度提供了新的可能。     

维生素B1-钼酸铵荧光光度法的研究与应用

1引言 维生素B1是人体维持正常代谢和机能必需的物质.用荧光光度法测定维生素B1的研究较为广泛.但因维生素B1的特殊性质,文献已报道的氧化测定体系,有的操作要求严格、繁琐;有的试剂毒性大,给环境带来污染;有的氧化时间较长,并需在沸水浴中加热.本文研究了钼酸铵与维生素B1的荧光反应,该氧化测定体系操作简便、快速,荧光强度稳定,测定线性范围宽,试剂无毒性.应用该方法测定了药品中的维生素B1,结果令人满意.     

荧光法测定食物中牛磺酸

利用荧光吸光光度法测定食物中牛磺酸含量,测定的相对标准偏差小于4.4%,标准加入回收率在92.0%-102%之间。样品经过前处理,避免了其它氨基酸的干扰。方法具有选择性好、操作简便等优点,适用于食物中牛磺酸的测定。     

Fluorometric Determination of Iron(Iii) with O-Hydroxyhydroquinonephthalein in the Presence of Brij 58

Abstract

A highly sensitive fluorescence reaction of iron(III) with o-hydroxyhydroquinonephthalein (Qnph) in the presence of various surfactants, and its application to the fluorophotometry of trace amounts of iron(III) is described. the method is based on the fluorescence quenching reaction between Qnph and iron(III) in the presence of Brij 58 at pH 3–4. the quenching calibration graph was linear over the range 0 – 300 ng per ml iron(III) by using fluorescence reaction at Em 525 nm with Ex 470 nm, and the iron(III) detection limit was 5 ng/ml. the proposed method is simple, rapid and does not involve heating or solvent extraction.     

Simultaneous Determination of Microgram Amounts of Platinum and Palladium in Presence of Base Metals by Isolation on Anion Exchange Resin-Loaded Paper and X-Ray Spectrometry

Abstract

A procedure for simultaneous determination of microgram amounts of platinum and palladium in solutions of base metals is described. The two metals are isolated on an anion exchange resin-loaded paper disk and determined by X-ray spectrometry. The method is applied to the determination of platinum and palladium in nickel matte.     

贵金属组试剂聚硫醚吸附性能和应用的研究

我们合成了类似文献报道的聚硫醚(PTE)。通过各种性能实验,确定了试剂结构为HO CH_2—S CH_2OH,对Au、Pd、Pt、Rh、Ir、Ru、Os的吸附率在98％以上,且不吸附常见的非贵金属。分离富集效果优于文献。像PTE能吸附全部8个贵金属且饱和吸附容量非常大的吸着剂,尚属少见。     

A critical review of neutron activation and tracer methods for the determination of the noble metals

Neutron activation methods for the seven noble metals are critically reviewed up to December, 1965. The methods deal with the determination of trace impurities in each noble metal as well as traces of noble metals in media such as organic material, fission product and other solutions, ores, alloys, minerals, silica and base metals. The need for confirmatory evidence in the case of such complex materials as ores is emphasized.     

Matrix separation of palladium and iridium by the cellulose anion exchanger Cellex T and the subsequent determination of palladium at theμg/g-level

The preconcentration and separation of palladium and iridium from base metals is studied with cellulose (Cellex T) and styrene-divinylbenzene (Varion AT 400) anion exchangers. In spite of lower capacity of Cellex T to chloride complexes of Pd and Ir, it allowed to get a higher preconcentration factor. Yields of 92–99% are achieved for Pd and 96–97% for Ir from the solutions containing great excess of base and heavy metals. Graphite furnace atomic absorption spectrometry is used for the final measurements. The procedure has been applied to the determination of palladium in natural samples.     

Titrimetric determination of gold by precipitation with hydroquinone

A rapid method for the volumetric determination of gold is described. Gold(III) is reduced to metal with excess of hydroquinone at room temperature and the excess is titrated with ceric sulphate. The effect of chloride ion on the reducibility of gold(III) to the metal is discussed. This method can be successfully employed for the determination of gold in presence of certain base and noble metals. The method was successfully adopted for the determination of gold in a commercial sample.     

The extraction of noble metals with n-octylaniline

The extraction of noble metals with n-octylaniline at varying concentrations of different mineral acids was studied, and optimum conditions for their separation from the base metals commonly present in platinum-bearing materials were established. The successful application of the procedure to the analysis of mattes, sludges, and flotation concentrates is shown. The lower limit of determination in such materials is 100 ppm for Pt and Ir, 40 ppm for Ru, and 10 ppm for Pd and Rh.     

罗丹明类化合物作为分析试剂的应用进展

对近年来罗丹明类化合物作为分析试剂的应用进展作出评述，主要涉及此类试剂在光度法、化学发光法、荧光光度法及共振散射光谱法等领域中的应用近况，引用文献87篇。