| 1,3,5,7-四甲基-2,6-二乙酯基-8-(3’,4’-二氨苯基)-二氟化硼-二吡咯甲烷荧光光度法灵敏测定血液中亚硝基硫醇 |
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| 引用本文： | 许春萱,黄克靖,牛德军,谢宛珍.1,3,5,7-四甲基-2,6-二乙酯基-8-(3’,4’-二氨苯基)-二氟化硼-二吡咯甲烷荧光光度法灵敏测定血液中亚硝基硫醇[J].化学通报,2009,72(10). |
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| 作者姓名： | 许春萱 黄克靖 牛德军 谢宛珍 |
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| 作者单位： | 信阳师范学院化学化工学院,信阳,464000 |
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| 基金项目： | 国家自然科学基金项目，中国博士后科学基金项目，河南省科技厅自然科学基金项目，河南省教育厅自然科学基金项目 |
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| 摘 要： | S-亚硝基硫醇在生物体内具有传递信号、改变蛋白质的结构和功能、阻止有害物质对细胞的伤害等重要的生理作用.建立了一种灵敏、简单的应用荧光探针1,3,5,7-四甲基-2,6-二乙酯基-8-(3',4'-二氨苯基)-二氟化硼-二吡咯甲烷标记并测定S-亚硝基硫醇的荧光光度法:在1.0×10-4mol/L Hg2+存在下,荧光探针与S-亚硝基硫醇在30℃反应15min生成衍生物,在激发和发射波长分别为500nm和510nm处测定其荧光强度.荧光强度和S-亚硝基硫醇的浓度在(8.0～800.0)×10-9mol/L范围内成良好的线性关系.当信噪比等于3时,方法的检出限为6.0×10-10mol/L.该方法对健康人与心血管疾病患者血液中S-亚硝基硫醇的测定的加标回收率为97.46%～103.10%.
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| 关 键 词： | 荧光探针 S-亚硝基硫醇 荧光光度法 |
Sensitive Determination of S-Nitrosothiols in Blood by Spectrofluorimetry using 1,3,5,7-Tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-Difluoroboradiaza-s-indacence as Probe |
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| Xu Chunxuan,Huang Kejing,Niu Dejun,Xie Wanzhen.Sensitive Determination of S-Nitrosothiols in Blood by Spectrofluorimetry using 1,3,5,7-Tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-Difluoroboradiaza-s-indacence as Probe[J].Chemistry,2009,72(10). |
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| Authors: | Xu Chunxuan Huang Kejing Niu Dejun Xie Wanzhen |
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| Institution: | College of Chemistry and Chemical Engineering;Xinyang Normal University;Xinyang 464000 |
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| Abstract: | S-nitrosothiols (RSNO) is an extremely important and versatile messenger in the biological system. It can transport signal,change the structure and function of protein and prevent damage of deleterious substance to cells in vivo. In this work,a spectrofluorimetric method for determining RSNO is proposed. An excellent fluorescence probe 1,3,5,7-tetramethyl?-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence is used to label RSNO. The derivatization reaction performs in aqueous medium at 3... |
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| Keywords: | Fluorescence probe S-nitrosothiols Spectrofluorimetry |
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