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采用荧光光谱法和紫外-可见分光光度法研究了变色酸与牛血清白蛋白之间的相互作用。结果表明:变色酸对牛血清白蛋白有较强的荧光猝灭作用。根据Stern-Volmer方程得到了荧光猝灭常数,并判断由于与变色酸反应而导致牛血清白蛋白的荧光猝灭属于静态猝灭。采用Lang-muir单分子吸附模型计算了结合常数和结合位点数。从计算得到的热力学参数ΔH和ΔS推断了变色酸与血清白蛋白反应的作用力为氢键和范德华力。 相似文献
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用荧光光谱和共振光散射光谱研究甲硝唑与牛血清白蛋白的相互作用 总被引:2,自引:0,他引:2
用荧光光谱和共振光散射光谱对甲硝唑与牛血清白蛋白的作用进行了对比研究, 测定了该反应的结合常数、结合位点数. 探讨了甲硝唑对牛血清白蛋白荧光和共振光散射猝灭的机理. 利用热力学参数确定了分子间的作用力性质;根据非辐射能量转换机制, 确定了甲硝唑-牛血清白蛋白间的结合距离. 采用同步荧光技术考察了甲硝唑对牛血清白蛋白构象的影响. 相似文献
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采用多种光谱技术对喜树碱和牛血清白蛋白的相互作用进行了研究.结果表明喜树碱和牛血清白蛋白可形成基态复合物,引起牛血清白蛋白内源荧光猝灭.通过计算获得了二者在不同温度下的结合常数及结合位点数.根据喜树碱和牛血清白蛋白结合的热力学参数,确定了二者之间主要为疏水作用力.根据F(o)rster非辐射能量转移理论确定了喜树碱和牛血清白蛋白的作用距离.同步荧光光谱显示喜树碱主要与蛋白中色氨酸残基发生相互作用,改变其周围的局部构象.红外光谱提示喜树碱可引起蛋白的构象发生改变,α-螺旋二级结构减少. 相似文献
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邓凡政 《中国无机分析化学》2014,4(3):71-74
红外光谱和X射线衍射分析表明甘氨酸与镧(Ⅲ)作用形成配合物。利用同步荧光光谱和荧光光谱探究了牛血清白蛋白(BSA)和甘氨酸镧(Ⅲ)配合物之间的相互作用。结果可知甘氨酸镧(Ⅲ)配合物与牛血清白蛋白的荧光猝灭为静态猝灭,根据双对数方程处理荧光猝灭数据得到了甘氨酸镧(Ⅲ)配合物与牛血清白蛋白在不同温度下的结合常数Kb和结合位点数n。热力学数据表明配合物与BSA作用主要是疏水作用力。利用同步荧光光谱法研究了甘氨酸镧(Ⅲ)配合物对于牛血清白蛋白的构象影响。 相似文献
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反白藜芦醇与牛血清白蛋白相互作用的多光谱法研究 总被引:5,自引:3,他引:2
利用荧光技术研究了抗癌药物反白藜芦醇与牛血清白蛋白的相互作用,结果证实,反白藜芦醇对牛血清白蛋白有很强的荧光猝灭作用,猝灭类型为静态猝灭.计算了反白藜芦醇与牛血清白蛋白在300、310和320 K时的结合常数、结合位点、结合距离和热力学常数等参数.由热力学参数推断两者结合过程中,起主导作用的是范德华力或氢键.同步荧光光谱和红外光谱同时说明两者结合前后蛋白质的结构发生了变化.研究还表明,金属离子Fe3 、Cu2 和Zn2 的加入使反白藜芦醇与牛血清白蛋白之间的结合常数和结合位点减小. 相似文献
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Yu M Ding Z Jiang F Ding X Sun J Chen S Lv G 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,83(1):453-460
The interaction between bovine serum albumin (BSA) and pegylated puerarin (Pur) in aqueous solution was investigated by UV-Vis spectroscopy, fluorescence spectroscopy and circular dichroism spectra (CD), as well as dynamic light scattering (DLS). The fluorescence of BSA was strongly quenched by the binding of pegylated Pur to BSA. The binding constants and the number of binding sites of mPEG(5000)-Pur with BSA were 2.67±0.12 and 1.37±0.05 folds larger after pegylating, which were calculated from the data obtained from fluorescence quenching experiments. The enthalpy change (ΔH) and entropy change (ΔS) were calculated to be 4.09 kJ mol(-1) and 20.01 J mol(-1) K(-1), respectively, according to Van't Hoff equation, indicating that the hydrophobic force plays a main role in the binding interaction between pegylated Pur and BSA. In addition, the negative sign for Gibbs free energy change (ΔG) implies that the interaction process is spontaneous. Moreover, the results of synchronous fluorescence and CD spectra demonstrated that the microenvironment and the secondary conformation of BSA were changed. Comparing with Pur, all our data collected indicated that pegylated Pur interacted with BSA in the same way as that of Pur, but docked into the hydrophobic pocket of BSA with more accessibility and stronger binding force. DLS measurements showed monomethoxy polyethylene glycol (mPEG) have an effect on BSA conformation, and revealed that changes in BSA size might be due to increases in binding constant and the absolute values of ΔG after Pur pegylation. 相似文献
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The present work reported the investigations on the interaction between a triphenylmethane industrial dye—crystal violet (CV)—and bovine serum albumin (BSA) by spectroscopic methods and molecular docking calculation. The static quenching mechanism of the intrinsic fluorescence of BSA by CV was deduced by the fluorescence measurements and the ground-state complex formation was confirmed from the UV-vis spectra. The site maker competition binding experiments together with the molecular docking showed that the CV molecule specifically bound on the subdomain IIA of BSA. The obtained values of thermodynamic properties of binding suggested that the hydrophobic interaction was dominated as suggested by molecular docking results that the CV molecule was surrounded by hydrophobic amino acid residues. The conformation change of BSA in the binding process was detected by circular dichroism spectra and Fourier-transform infrared (FTIR) spectra and also reflected by the size change of BSA from the measurements by dynamic light scattering (DLS). 相似文献
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利用荧光猝灭法和动态光散射法测定尿素-水混合溶剂中牛血清白蛋白(BSA)与荧光素的结合距离和BSA的流体动力学半径, 并通过分析BSA和荧光素在BSA-尿素-水和荧光素-尿素-水三元体系以及BSA-荧光素-尿素-水四元体系中荧光光谱的变化, 探讨尿素与蛋白质分子在水溶液中相互作用的机理及其对蛋白质构象的影响. 结果显示, BSA的3个结构域在尿素-水混合溶剂中具有不同的稳定性, 其中结构域III在尿素-水混合溶剂中是不稳定的, 而结构域I和结构域II分别在尿素浓度大于3.0和4.0 mol8226;L-1的混合溶剂中发生去折叠. 试验发现, BSA结构域II在低于去折叠浓度的尿素-水混合溶剂中形成更为紧密的构象, 这一现象可以归因于尿素与BSA结合引起的“蛋白质粘稠效应” 相似文献
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Yu X Yang Y Shiyu L Yao Q Heting L Xiaofang L Pinggui Y 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,83(1):322-328
The interaction between imidazo[2,1-b]thiazole (IMTZ) and bovine serum albumin (BSA) was analyzed by fluorescence and ultraviolet spectroscopy at 302 and 310 K under simulative physiological conditions. The results show that IMTZ can effectively quench the intrinsic fluorescence of BSA via static and dynamic quenching. The binding constant, binding sites of IMTZ with BSA were calculated. According to the F?rster non-radiation energy transfer theory, the average binding distance between IMTZ and BSA was obtained. What's more, the synchronous fluorescence spectra indicated that the conformation of BSA has been changed. The results provided the information for the binding of IMTZ to BSA, and the influences of substituent group on the interaction were also discussed. 相似文献
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Hui Lin Jingfeng Lan Min Guan Fenling Sheng Haixia Zhang 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2009,73(5):936-941
The mechanism of interaction between mangiferin (MA) and bovine serum albumin (BSA) in aqueous solution was investigated by fluorescence spectra, synchronous fluorescence spectra, absorbance spectra and Fourier transform infrared (FT-IR) spectroscopy. The binding constants and binding sites of MA to BSA at different reaction times were calculated. And the distance between MA and BSA was estimated to be 5.20 nm based on Föster's theory. In addition, synchronous fluorescence and FT-IR measurements revealed that the secondary structures of the protein changed after the interaction of MA with BSA. As a conclusion, the interaction between the anti-diabetes Chinese medicine MA and BSA may provide some significant information for the mechanism of the traditional chinese medicine MA on the protein level to cure diabetes or other diseases. 相似文献
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The interactions between potassium perfluorooctanesulfonate (PFOS) and bovine serum albumin (BSA) were studied by fluorescence spectroscopy. The association constants between PFOS and BSA were obtained by fluorescence enhancing and fluorescence quenching respectively. Furthermore, fluorescence quenching was studied at different temperatures, and the binding constant was also determined by the method of fluorescence quenching. According to the thermodynamic parameters, the main binding force could be judged. The experimental results revealed that BSA and PFOS had strong interactions. The mechanism of quenching belonged to dynamic quenching and the main sort of binding force was hydrophobic force. IR-spectra proved the interaction changed the conformation of BSA. 相似文献
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Zhang LN Wu FY Liu AH 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,79(1):97-103
A new compound, 2,5-di-[2-(4-hydroxy-phenyl)ethylene]-terephthalonitrile (DHPEPN), was synthesized. The interaction between bovine serum albumin (BSA) and DHPEPN in Tris-HCl buffer solution (pH 7.4) was investigated using fluorescence and UV-vis absorption spectroscopy. The mechanism of BSA fluorescence quenched by DHPEPN is discussed according to the Stern-Volmer equation. The binding constant and the thermodynamic parameters ΔH, ΔS, ΔG at different temperatures were calculated. The results indicate that the van der Waals interaction and hydrogen bonding play major roles in the binding process. The distance between BSA and DHPEPN is estimated to be 3.59 nm based on the F?rster resonance energy transfer theory. The spectral changes of synchronous fluorescence and three-dimensional fluorescence suggest that both of the microenvironment of DHPEPN and the conformation of BSA are changed during binding between DHPEPN and BSA. 相似文献
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光谱法与分子模拟研究胡椒碱对牛血清白蛋白的键和作用 总被引:5,自引:1,他引:4
利用荧光光谱法及紫外吸收光谱法结合计算机模拟技术研究了在模拟生理条件下胡椒碱(piperine)与牛血清白蛋白(Bovine Serum Albumin, BSA)的相互作用. 根据荧光猝灭的有关方程分别求得不同温度下(298, 308和318 K)药物与蛋白相互作用的结合常数、结合位点数及键合距离. 实验所得到的热力学参数(ΔH1256;=-9.55 kJ/mol, ΔS1256;=46.75 J8226;mol-18226;K-1)表明维持药物与蛋白质的相互作用力主要是疏水作用和静电作用. 分子模拟的结果显示了胡椒碱与BSA的键合机理和键合模式, 表明维持药物与蛋白质的相互作用力主要是疏水作用和氢键(位于氨基酸残基His 242, Arg 222和Arg 218位). 此外, 基于胡椒碱的荧光猝灭效应, 首次探讨了药物-蛋白质体系的几种物理化学参数, 包括电荷密度(δ)、离解常数(Kd)及量子产率(F)的变化效应. 相似文献
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5,7-二羟基-4'-甲氧基二氢黄酮与牛血清白蛋白的相互作用研究 总被引:1,自引:0,他引:1
应用荧光光谱、紫外吸收光谱和核磁共振波谱研究了5,7-二羟基-4'-甲氧基二氢黄酮(ISO)与牛血清白蛋白(BSA)分子间的相互作用. 研究表明: ISO对BSA内源性荧光的猝灭机制属于ISO和BSA形成化合物所引起的静态猝灭; 二者的结合常数为7.41×1011 L/mol, 结合位点数为1.98. ISO与BSA作用的活性部位为其分子内的7-OH和5-OH, 且7-OH活性强于5-OH, 并且随着ISO浓度增大, BSA的构象发生了变化. 相似文献