| 变色酸与牛血清白蛋白的相互作用 |
| |
| 引用本文： | 李咏玲,杜慧玲,程芳琴.变色酸与牛血清白蛋白的相互作用[J].理化检验(化学分册),2012(4):396-399. |
| |
| 作者姓名： | 李咏玲 杜慧玲 程芳琴 |
| |
| 作者单位： | 山西农业大学文理学院;山西大学化学化工学院;山西大学资源与环境研究所 |
| |
| 基金项目： | 国家自然科学基金(21072119);山西省青年科技基金(2010021010-2) |
| |
| 摘 要： | 采用荧光光谱法和紫外-可见分光光度法研究了变色酸与牛血清白蛋白之间的相互作用。结果表明:变色酸对牛血清白蛋白有较强的荧光猝灭作用。根据Stern-Volmer方程得到了荧光猝灭常数,并判断由于与变色酸反应而导致牛血清白蛋白的荧光猝灭属于静态猝灭。采用Lang-muir单分子吸附模型计算了结合常数和结合位点数。从计算得到的热力学参数ΔH和ΔS推断了变色酸与血清白蛋白反应的作用力为氢键和范德华力。
|
| 关 键 词： | 变色酸 牛血清白蛋白 相互作用 荧光光谱法 分光光度法 |
Interaction of Chromotropic Acid and Bovine Serum Albumin |
| |
| LI Yong-ling,DU Hui-ling,CHENG Fang-qin.Interaction of Chromotropic Acid and Bovine Serum Albumin[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(4):396-399. |
| |
| Authors: | LI Yong-ling DU Hui-ling CHENG Fang-qin |
| |
| Institution: | 1.College of Arts and Science,Shanxi Agricultural University,Taigu 030801,China; 2.College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China; 3.Research Institute of Resource and Environments,Shanxi University,Taiyuan 030006,China) |
| |
| Abstract: | Mechanism of the interaction between chromotropic acid(CMT) and bovine serum albumin(BSA) was studied by fluorospectrometry and UV-Vis spectrophotometry.It was found that significant quenching of fluorescence of BSA appeared due its reaction with CMT.As judged from the fluorescence quenching constants obtained by Stern-Volmer equation,fluorescence quenching of BSA by CMT was attributed to static quenching.The binding constants and number of binding sites were found by applying the model of Langmuir monolayer adsorption.Values of thermodynamic parameters ΔH and ΔS were calculated and based on these results,the binding force of the reaction was recognized to be hydrogen bond and Vander Waals force. |
| |
| Keywords: | Chromotropic acid Bovine serum albumin Interaction Fluorospectrometry Spectrophotometry |
| 本文献已被 CNKI 等数据库收录！ |
