共查询到19条相似文献,搜索用时 625 毫秒
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以白细胞(WBC)作为原料,经过固定、分散,制备尿沉渣WBC标准物质。分别采用F检验和回归曲线法评估标准物质的均匀性和稳定性,使用显微镜计数法进行定值,并对标准物质进行不确定度评定。研制的标准物质均匀性和稳定性良好,于2~8℃避光可保存180天。白细胞标准物质水平1,2,3特性值分别为(1 061.1±86.97),(548.8±55.96),(220.5±26.83) cells/μL(k=2)。该系列制备物可作为临床尿液有形成分白细胞分析仪器校准,测量过程质量控制及分析方法的确认和评价检定用标准物质。 相似文献
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《化学分析计量》2015,(4)
介绍高浓度血清雌二醇标准物质的研制。采集的全血不加任何添加剂和抗凝剂,离心分离出血清,分装。采用同位素稀释–液相色谱串联质谱法(ID–LC–MS/MS)对高浓度血清雌二醇标准物质进行定值及均匀性和稳定性检验,对定值不确定度进行了评定。结果表明,高浓度血清雌二醇标准物质定值结果为3.06 ng/g,扩展不确定度为0.08 ng/g(k=2),均匀性和稳定性符合国家标准物质技术要求。采用IFCC RELA比对样品和欧洲标准局标准物质血清17β-雌二醇标准物质对定值方法进行确认,测量结果的标准偏差在该标准物质量值的不确定度范围内。研制的标准物质量值经过日本计量院验证,测量结果为(3.03±0.1)ng/g。该标准物质的量值具有溯源性和准确性。 相似文献
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江苏省分析测试协会色谱专业委员会于 2 0 0 2年 5月 17日在南京市江苏省生产力促进中心正式成立。来自全省科研院所、高等院校、中小企业等从事食品、医药、环境、农药等行业的色谱技术人员约 10 0人参加了成立大会。大会由江苏省分析测试协会秘书长夏春阳主持 ,常务理事长姚义刚到会祝贺并发表了讲话。会议选举袁倚盛教授为主任委员 ,戴军、赵厚民、江夕夫和练鸿振为副主任委员。该色谱专业委员会成立后将为江苏省广大色谱类仪器分析测试技术人员组织及举办有关色谱 /质谱仪器的分析测试学术报告会、分析测试技术交流会和分析仪器展示会 ;… 相似文献
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McCarron P Emteborg H Nulty C Rundberget T Loader JI Teipel K Miles CO Quilliam MA Hess P 《Analytical and bioanalytical chemistry》2011,400(3):821-833
The development of multi-analyte methods for lipophilic shellfish toxins based on liquid chromatography–mass spectrometry
permits rapid screening and analysis of samples for a wide variety of toxins in a single run. Validated methods and appropriate
certified reference materials (CRMs) are required to ensure accuracy of results. CRMs are essential for accurate instrument
calibration, for assessing the complete analytical method from sample extraction to data analysis and for verifying trueness.
However, CRMs have hitherto only been available for single toxin groups. Production of a CRM containing six major toxin groups
was achieved through an international collaboration. Preparation of this material, CRM-FDMT1, drew on information from earlier
studies as well as improved methods for isolation of toxins, handling bulk tissues and production of reference materials.
Previous investigations of stabilisation techniques indicated freeze-drying to be a suitable procedure for preparation of
shellfish toxin reference materials and applicable to a wide range of toxins. CRM-FDMT1 was initially prepared as a bulk wet
tissue homogenate containing domoic acid, okadaic acid, dinophysistoxins, azaspiracids, pectenotoxin-2, yessotoxin and 13-desmethylspirolide
C. The homogenate was then freeze-dried, milled and bottled in aliquots suitable for distribution and analysis. The moisture
content and particle size distribution were measured, and determined to be appropriate. A preliminary toxin analysis of the
final material showed a comprehensive toxin profile. 相似文献
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The reliability of measurement results is essential for forming a common database of a laboratory network, because a well-maintained and consistent database is the crucial point of countrywide monitoring. Reference materials are important tools in realizing some aspects of quality assurance; they are especially useful in harmonizing work within the network. Among usual types of reference materials in radio analytics solutions, matrix reference materials and some special reference objects are used. All classes of reference materials should be used depending on the purpose of the demonstration of quality, even in-house reference materials. Interlaboratory measurement comparison and performance evaluation programs play important quality assurance role in radio analytical laboratories. Fortunately, nowadays, the main task is to determine a very low radioactivity concentration in the environment; therefore, pre-concentration is necessary. Generally, the radionuclide bearing natural materials collected from sites where there had been sufficient time for natural processes to redistribute various chemically different species of radionuclides are more reliable reference materials than spiked materials—the main difference is the chemical bounding which is crucial from the point of view of the bioavailability. The need of reference material is summarized according to the intended use, like quality control, measurement validation, and instrument calibration. 相似文献
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The production of stable reference materials with incurred residues of veterinary drugs is necessary for the validation of methods of analysis, including the determination of critical performance characteristics. A reference material for trenbolone in bovine urine was produced and the long-term stability was tested. From a pilot 16 week stability study on seven batches containing different additives it was concluded that the use of preservatives does not improve the stability of the residue. A final batch of reference material of 800 vials each containing 5 ml of urine with a target concentration of 5 micrograms l-1 was prepared. The homogeneity and long-term stability of the material were tested. The material was found to be homogeneous. Based on the results of a 52 week stability study it was concluded that the material is stable, using the current analytical methodology. For the development of reference materials, highly accurate and precise analytical methods are necessary. However, the current analytical methodology is not suitable for full evaluation and certification. Currently, a new LC-MS method is being developed. After validation of this method, the stability and homogeneity study will be repeated. 相似文献
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I. Kuselman Boris Anisimov Avinoam Shenhar Alex Lepek 《Accreditation and quality assurance》1997,2(5):234-237
The approach to validation of a computer program for an analytical instrument as a component of the analytical method (using
this instrument with the program) is discussed. This approach was used for validating a new program for atomic absorption
analysis. The validation plan derived from this approach was based on minimising the influence of all steps of the analytical
procedure on the analytical results obtained by the method. In this way significant changes in the results may be caused only
by replacement of the previous program by the new one. The positive validation conclusion was based on the comparison of the
results of the analysis of suitable reference materials obtained with the new program and with its precursor in the same conditions,
and also on comparison of their deviations from the accepted reference values for these materials, with the corresponding
uncertainties.
Received: 25 January 1997 Accepted: 14 March 1997 相似文献
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Stable isotope ratios are reported in the literature in terms of a deviation from an international standard (delta-values). The referencing procedures, however, differ from instrument to instrument and are not consistent between measurement facilities. This paper reviews an attempt to unify the strategy for referencing isotopic measurements. In particular, emphasis is given to the importance of identical treatment of sample and reference material ('IT principle'), which should guide all isotope ratio determinations and evaluations. The implementation of the principle in our laboratory, the monitoring of our measurement quality, the status of the international scales and reference materials and necessary correction procedures are discussed. 相似文献
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Yuichi Takaku Takuma Hayashi Ikuko Ota Hidenao Hasegawa Shinji Ueda 《Analytical sciences》2004,20(7):1025-1028
An analytical method for trace levels of iron in a seawater sample using isotope dilution ICP-MS was developed. Preconcentration of iron and the removal of major elements in seawater such as alkali and alkaline-earth elements can be carried out quickly using a chelating resin disk by adjusting the sample pH to 3. The collision cell option of the ICP-MS instrument method was used to improve the performance of the instrument for iron measurements since ArO and ArN interferences could be reduced using this analytical method. About 4 ml min(-1) helium, as the collision gas, were introduced into the cell. 40Ar14N and 40Ar16O which interfere with 54Fe and 56Fe in water had their amounts decreased by 5 orders of magnitude. Then, the isotope dilution method was used for iron determination below ng g(-1) level of trace iron in four environmental reference materials (river water standard sample JAC-0031 (Japan Soc. for Analytical Chemistry), estuarine standard sample SLEW-2 (NRC Canada) and seawater standard samples CASS-3 and NASS-5 (NRC Canada)) were measured. Good agreement between analytical results and certified values of reference materials was obtained, which confirmed the effectiveness of this method. 相似文献
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Capillary electrophoresis (CE) is a well-established and frequently used technique in the pharmaceutical industry. Therefore an appropriate analytical instrument qualification (AIQ) is required for quality assurance. AIQ forms the basis of a quality management followed by analytical method validation, system suitability tests (SSTs) and quality control checks. Two parts of the AIQ, namely the operational qualification (OQ) and the performance qualification (PQ) are of particular interest in the daily routine of the laboratory. A new concept for OQ and PQ was developed to assure the correct function of a CE system. The significance of each parameter, possible test methods as well as acceptance criteria will be presented and discussed in detail. Especially temperature adjustment by the cooling system and the voltage supply must be tested for accurate and precise operation. The detector noise, wavelength accuracy and detector linearity have to be checked as well. Finally, the injection linearity, accuracy and precision need to be qualified. The proposed set of qualification procedures is easy to implement and was already tested on five CE instruments from three different manufacturers. A time- and cost-saving continuous PQ was derived, using results from method-specific SSTs and some additional experiments. This holistic concept continuously surveys the most relevant parameters, hence assuring the suitability of the used instruments and decreasing their downtimes. 相似文献
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A new type closed digestion vessel was constructed for digestion of biological samples. In this the vapour pressure can be maintained on moderated level (150-300 kPa) by means of an internal cooling spiral. During the operation the reflux of the condensed acid and water vapour continuously renews the liquid phase over the sample. By this way a less expensive microwave system may be applied. The performance of this instrument is practically equal to the commercial systems. The digestion time of plant and tissue samples is 5-10 min and the analytical results for reference materials are in good agreement with the reference values. 相似文献