X射线照射Hep-2细胞对其培养基吸收光谱的影响

采用分光光度计分别测量了受不同剂量X射线照射后继续培养48h时的人上皮样喉癌细胞株Hep-2的培养基RPMI1640的紫外吸收光谱,分析了该培养基中蛋白质的紫外吸收特性.结果发现:各组培养基的吸收光谱存在明显的差别,新鲜培养基RPMI1640(10%胎牛血清)233nm处的吸收峰在细胞生长过程中位移至235nm,275nm处的吸收峰位移至278nm.反映出各组培养基中芳香族氨基酸中各种氨基酸如色氨酸、酪氨酸、苯丙氨酸的含量的变化,说明癌细胞在生长过程中对这几种氨基酸的改变不是按等量比的.实验还发现细胞培养基的吸收光谱强度与各剂量组细胞数基本成正相关,进而与细胞所受X射线照射的剂量有内在的联系.这些结果将为利用光谱技术研究人喉癌最佳X射线放射剂量奠定基础.     

细胞培养基的吸收光谱研究

使用日本岛津UV 310 1分光光度计分别测量培养了宫颈癌细胞 (Hela)和鼻咽癌细胞 (CNE)的RP MI 16 4 0培养基和DMEM高糖培养基的紫外吸收光谱 ,分析了培养基中蛋白质的紫外吸收特性。RPMI16 4 0培养基的 2 2 7nm吸收峰在培养细胞生长过程中位移至 2 2 2或 2 18nm ,2 78nm的吸收峰位移至 2 80nm ;而DMEM高糖培养基 2 2 4nm吸收峰在培养细胞生长过程中位移至 2 2 1nm附近 ,2 78nm吸收峰基本没有位移。这些位移说明培养基中芳香族氨基酸中各种氨基酸如色氨酸和酪氨酸的含量有变化。也就是说 ,癌细胞在生长过程中对色氨酸和酪氨酸的消耗不是按等量比的。实验也说明Hela和CNE在生长过程中 ,对RPMI 16 4 0培养基和DMEM高糖培养基中氨基酸的消耗是不同的。实验结果为癌细胞生长过程中所需培养基中氨基酸的含量提供了依据     

曲古菌素A作用下HeLa细胞的吸收光谱分析

用紫外分光光度计采集了人宫颈癌细胞经不同剂量的曲古菌素A（TSA）作用24h和48h后的吸收光谱。结果表明：细胞在260nm附近存在特征性的吸收峰,且200nmol/L的药物剂量会使HeLa细胞在260nm处的吸收值最大。该项工作找到了细胞经药物处理后吸收光谱与用药剂量之间的关系,为药物的筛选提供了新的思路。     

紫外激光照射引起的硅酸铅玻璃结构的变化

采用紫外-可见吸收光谱和电子自旋共振谱(ESR)对硅酸铅玻璃薄膜和体材料受紫外激光照射前后的结构变化进行了研究。研究发现：266 nm的紫外激光照射硅酸铅玻璃体材料时,能使其Urbach能量增大,即玻璃结构的无序性增大。电子自旋共振谱研究表明,266 nm激光照射不会在硅酸铅玻璃中产生顺磁缺陷中心, 也不会对硅酸铅玻璃薄膜在235 nm附近的吸收峰产生影响,但用248 nm紫外激光照射则观察到了235 nm吸收峰的光漂白现象。     

UV照射大肠杆菌对其吸收光谱的影响

探讨了样品池溶液及分光光度计内置紫外光对大肠杆菌吸收光谱测定的影响。发现样品池溶液(2.8 mL双蒸水+0.1 mL生理盐水+0.1 mL LB培养基)较为合理,既给细菌提供了有利的生存环境,又对细胞的紫外吸收影响较小;研究同时发现在257 nm波段150 s内细胞吸收强度基本没有变化,说明内置的紫外光不会影响试验测定结果。在此基础上,研究了UV照射0,20,40,60,80,120 s大肠杆菌吸收光谱的变化情况。结果显示随着UV照射剂量的增加,大肠杆菌吸收光谱存在一个先降后升再降的现象。该现象反映了辐射中细胞损伤死亡和修复存活的生物学效应。该研究从光谱学角度探讨了UV对大肠杆菌细胞的损伤作用,对利用光谱技术研究细胞的辐射损伤、死亡和修复具有一定参考意义。     

一种新型的蓝光发射材料δ-Alq3的制备与光谱分析

制备了一种新型的蓝光发射材料δ-Alq3,测试了其红外吸收光谱、X射线衍射谱、紫外-可见光吸收光谱、光致发光光谱和电致发光光谱,并通过与δ-Alq3的光谱性能的比较,研究了Alq3分子空间结构与光谱性能之间的关系.结果表明:δ-Alq3分子空间结构经过真空加热发生变化,生成对称性较差的δ-Alq3;分子空间结构的改变引起Alq3分子中酚环电子云密度的降低和相邻分子间的共轭效应的减弱,导致在紫外-可见吸收光谱中δ-Alq3长波段的π-π*电子跃迁吸收峰向短波方向的移动,在光致发光光谱中δ-Alq3的最大发射峰波长为480 nm,比α-Alq3的最大发射峰波长(515 nm)蓝移35 nm,在电致发光光谱中α-Alq3和δ-Alq3的最大发射峰波长都为520 nm.     

双掺杂LiNbO3:Ce:Cu晶体的光学性能

用提拉法生长出3种不同浓度的双掺杂LiNb03∶Ce∶Cu晶体,测试双掺杂晶体的红外吸收光谱、紫外可见光吸收光谱和X射线衍射谱,掺杂相对浓度较高时,紫外吸收边向长波方向红移,X射线谱峰半峰值宽度变小.测试掺杂晶体在生长态和氧化态时的衍射效率,氧化态的衍射效率比生长态衍射效率高,但响应时间较长.     

八丁氧基-2,3-萘酞菁铜(Ⅱ)的合成与UV光谱研究

采用分子碎片法合成了5,9,14,18,23,27,32,36-八丁氧基-2,3-萘酞菁铜,利用元素分析、红外及紫外可见光谱进行了表征。配合物的电子吸收光谱表明:新配制的氯仿溶液中萘酞菁铜的Q带H聚集体和单体的吸收峰在758、854nm处,5h后单体和H聚集体的吸收峰基本消失,出现了798和909nm的错位H/J聚集体以及960nm的J聚集体吸收,12h后主要以795nm的H聚集体形式存在。     

光谱学在金黄色海水珍珠鉴定中的应用

为了有效鉴别区分金黄色海水珍珠(简称金珠)和改色金珠,通过拉曼光谱和紫外可见吸收光谱对二者进行了检测分析.结果表明,拉曼光谱中,金珠的谱线稳定杂峰少,荧光背景弱,改色金珠的谱线杂峰多,荧光背景很强.0～1000 cm-1拉曼位移内金珠的光谱基线强度在1000以下,改色金珠的光谱基线强度在2000以上;金珠在拉曼位移275 cm-1处出现明显的拉曼特征峰,改色金珠275 cm-1处的拉曼特征峰基本被荧光谱线覆盖而变弱或消失;紫外可见吸收光谱中,金珠在200 nm～400 nm的近紫外区有284 nm,357 nm两个明显的紫外吸收峰.由于颜色处理过程中对珍珠表面破坏程度的不同,导致改色金珠在近紫外区无特征吸收,吸收峰发生红移(408 nm)或消失.     

中空Ag纳米球壳的制备及性能表征

 以改性聚苯乙烯微球为模板,采用化学镀法在聚苯乙烯微球表面包覆一层银,在四氢呋喃溶液中将聚苯乙烯微球溶解,得到中空Ag纳米球壳。采用扫描电镜、透射电镜和X射线衍射仪对样品进行了表征及分析,并用紫外可见分光光度计研究了粒子的光学性质。实验结果表明：运用此法成功地制备出中空Ag纳米球壳的内径为250 nm,壁厚约为15 nm,并且成功地使纳米粒子的紫外吸收光谱由600 nm红移至900 nm左右,实现了在可见光至近红外光区调节Ag纳米结构的吸收峰。     

Photocatalytic effect of anodic titanium oxide nanotubes on various cell culture media

The use of titanium dioxide (TiO₂) in photodynamic therapy for the treatment of cancer cells has been proposed following studies of cultured cancer cells. In this work, an ordered channel array of anodic titanium oxide (ATO) was fabricated by anodizing titanium foil. The ATO layer of nanotubes with diameters of 100 nm was made in NH₄F electrolyte by anodization. The photocatalytic effect of ATO was examined on various culture media by ultraviolet A (UV-A) (366 nm) irradiation. After UV-A irradiation of the ATO layer, redox potential of Tris-HCl buffer (pH 7.5) and dilute acrylamide solution increased instantaneously. The redox potential of the serum-containing RPMI1640 medium also increased dramatically, while that of serum-containing MEM and DMEM media increased slightly. The UVA-induced high redox potential was correlated with the greater ability to break down plasmid DNA strands. These phenomena suggest that a culture medium, such as RPMI1640, with a greater ability to produce free radical may be associated with a stronger photocatalytic effect of ATO on cultured cancer cells reported previously.     

Basic study on apoptosis induction into cancer cells U-937 and EL-4 by ultrasound exposure

Recently, the low invasive cancer treatments with small aftereffects have been considered. We are studying on the suppression methods of cancer cell proliferation with ultrasound. Cancer cells of mouse T lymphoma (EL-4) have been used in our study. The human histitocytic lymphoma cells (U-937) was used in this time. The cancer cells were cultured in a culture medium of RPMI1640. The standing wave acoustic field was formed in a water tank of our ultrasound exposure system by a vibrating plate driven with a Langevine type transducer. The U-937 and EL-4 were exposed to ultrasound in the acoustic field with spatial average acoustic intensity of 350 mW/cm(2) at 150 kHz. The viable rate of EL-4 decreased with the lapse of culture time after ultrasound exposure. U-937 did not show the remarkable decrease tendency. The proliferation of U-937 which exposed to ultrasound with 700 mW/cm(2) was suppressed. It can be thought that apoptosis was induced in the cancer cells in this condition. We observed the morphological change on the U-937 exposed to ultrasound with this condition. The morphological changes by apoptosis like the shrink of cells, formation of apoptotic bodies etc. can be observed with an optical microscope and a phase contrast microscope.     

浮游植物吸收光谱特征分析

文章选取了东海9个典型浮游植物赤潮种、优势种,在实验室2个温度(20,15 ℃)和2个光照(7 000, 1 100 lux)条件下进行培养,对不同温度、光照和生长周期条件下9种浮游植物的吸收光谱进行了研究。首先,将归一化的光谱数据组成的矩阵进行奇异值分解,采用第一特征值与所有特征值的和之比来衡量浮游植物吸收光谱的相似性,选取了25条能够表征9种浮游植物吸收光谱基本特征的代表谱。其中,等鞭金藻和柔弱角毛藻各1条,岛国大扁藻和旋链角毛藻各2条,裸甲藻3条,塔玛亚力山大藻、东海原甲藻、中肋骨条藻和聚球藻各4条。然后,进一步提取代表谱中具有较大判别能力的波长点,总共得到7个特征波长段(点),它们是340.5～420.5 nm波段、423.5～431 nm波段、440.5～525.5 nm波段和760.5,763.5,769.5,856.5 nm波长点;由此形成的特征谱,对量测光谱判别分析正确率达到80%。     

CdS量子点的酿酒酵母仿生合成及光谱表征

以酿酒酵母为载体,常温下利用仿生法成功合成了CdS量子点。荧光发射光谱、紫外吸收光谱以及荧光显微镜照片证明,该方法合成的CdS量子点的荧光发射峰位置在443nm,在紫外灯下能发蓝绿色荧光。透射电子显微镜(TEM)表征结果表明,该仿生法合成的CdS量子点为六方纤锌矿结构。以荧光发射和紫外吸收光谱为性能指标,考察了酿酒酵母生长时期、Cd2+的反应浓度以及反应时间等条件对合成CdS量子点的影响。当酿酒酵母处于生长稳定期初期时,与浓度为0.5mmol.L-1的Cd2+共培养24h后所合成的CdS量子点荧光最强。实验中观察到,换液培养可有效提高酿酒酵母合成CdS量子点的产量。     

A study on the best irradiation dose of X-ray for Hep-2 cells by Fourier transform infrared spectroscopy

Fourier transform infrared spectroscopy (FTIR) was employed to study the human epidermis larynx carcinoma cell lines (Hep-2) which were irradiated by different doses of X-ray.The results show that (1) the irradiation of X-ray damages the structure of the CH3 groups of the thymine in DNA,which restrains the reproduction of Hep-2 cells effectively,(2) the 8 Gy dose of X-ray irradiation changes the framework and the relative contents of some proteins,lipids and the nucleic acid molecules intercellular in the greatest degree,and (3) the 8 Gy dose of X-ray irradiation is the best irradiation dose for lowering the degree of the cancerization of Hep-2 cells according to the criteria for the degree of the cancerization reported recently.Meanwhile,the apoptosis of these cells were detected by using flow cytometry (FCM) primarily.It shows that the apoptotic ratio of the Hep-2 cells depends on the irradiation dose to some extent,but is not linearly.And the apoptotic ratio of the 12 Gy dose group is the maximum (20.36%),but the apoptotic ratios of the 2 to 8 Gy dose groups change little.     

On the Feasibility of an In Vitro Noninvasive Absorbance-Based Cell Proliferation Analysis Technique,Using Cell Culture Media

The preliminary results of a study on the applicability of using spectrophotometry of the culture medium toward understanding of cells' response to different environments is reported. The cells are cultivated on polydimethylsiloxane (PDMS) substrates and physicochemically manipulated by varying the substrate stiffness and the dose of surface plasma treatment. By growing cervical cancer (HeLa) cells on PDMS with varying stiffness and plasma treatment levels, we tested the hypothesis that the influence of substrate stiffness and treatment can be detected through the spectrophotometry of a microvolume of cell culture medium. The results imply an opportunity of using the spectrophotometry of culture media with an integrated minimal sample retention system as a practical and noninvasive alternative to traditional bioassays requiring cells detachment.     

水溶胶CdSe/CdS核/壳结构纳米晶制备及光学性质的研究

以巯基乙酸为稳定剂在水溶液中合成了水溶胶CdSe/CdS核/壳结构的量子点,利用X射线粉末衍射(XRD)和X射线光电子能谱(XPS)对量子点结构进行了表征;并对化学组成和尺寸分布进行了研究。通过紫外-可见吸收光谱、激发光谱与发射光谱研究了它们的发光特性。     

余弦光栅硅薄膜太阳能电池光吸收分析

设计了一维双层余弦共形光栅结构的单晶硅薄膜太阳能电池.利用时域有限差分法模拟计算了双层余弦共形光栅结构和对照组结构的吸收光谱;利用归一化光吸收密度的概念,定量分析了300~700nm和700~1 100nm两个波段的光吸收效率.结果表明双层共形光栅结构具有更好的全波段吸收效率,且在长波段余弦光栅比矩形光栅具有更好的光捕获和吸收作用.利用光吸收增强谱和电磁场强度分布图,分析了余弦光栅在长波段的吸收增强机理.通过计算短路电流密度,发现双层余弦共形光栅结构比平板结构太阳能电池的短路电流密度提高了79.5%,余弦光栅结构比矩形光栅结构的短路电流密度提高了8.5%.