Chemiluminescence Sensor for the Determination of Hydroxylamine by Electrostatically Immobilizing Luminol and Periodate

IntroductionAnalytical methods based on chemilumi-nescence( CL ) reactions have received considerableattention for theirapplication in various fields,ow-ing to their extremely high sensitivity along withother advantages such as wide linear dynamicrange,simple instrumentation,easy miniaturiza-tion and coupling to various separation tech-niques[1— 6] .CL sensors have been developed for be-ing used as the continuous and real- time monitorsof inorganic,organic,biological and pharmaceuti-cal comp…     

流动注射化学发光抑制法测定阿魏酸钠

提出了Fe2+-H2O2-亚甲基蓝化学发光新体系并用于阿魏酸钠的测定。实验发现,在酸性介质中,Fe2+-H2O2体系可与亚甲基蓝反应产生极强的化学发光,阿魏酸钠对此化学发光具有显著的抑制作用。据此,结合流动注射技术,建立了阿魏酸钠化学发光分析新方法。研究了影响化学发光强度的因素,化学发光信号的降低值(ΔI)与阿魏酸钠浓度在4.5×10-6～4.5×10-5mol/L范围内呈良好的线性关系,方法的检出限为7.0×10-7mol/L。对4.8×10-6mol/L的阿魏酸钠进行了11次平行测定,其RSD=0.8%,该法已用于片剂中阿魏酸钠含量的测定。     

流动注射化学发光植物组织传感器测定草酸盐

草酸是尿道结石主要成因之一 .统计结果表明 ,90 %以上结石均含草酸钙 .回肠病、口角性肠胃炎及脂肪吸收不良均可引起尿液中草酸的增加 .草酸盐的测定在临床上具有重要意义 .微量草酸测定的传统方法是变色酸比色法或偶氮化合物比色法[1 ] .测定草酸盐的方法还有原子吸收光谱法[2 ] 、高效液相色谱法[3] 、离子色谱法[4] 、光度法[5] 和草酸氧化酶法[6] 等 .这些方法或操作复杂、耗时 ,或灵敏度低 ,使其应用受到限制 .本文提出一种测定草酸盐的新方法 ,其原理为草酸 +Ｏ2草酸氧化酶 ＣＯ2 +Ｈ2 Ｏ2 ,Ｌｕｍｉｎｏｌ+Ｈ2 Ｏ2 ｈｖ　　将含有草…     

流动注射化学发光法快速测定尿液中左亚叶酸钙

在碱性介质中, 左亚叶酸钙对鲁米诺-K3Fe(CN)6体系有显著的增敏作用, 据此, 建立了一种简单、快速测定左亚叶酸钙的流动注射化学发光新方法. 在优化的实验条件下, 该法测定左亚叶酸钙的线性范围为5.0×10-8～1.0×10-5 g/mL; 检出限(3σ)为2.0×10-8 g/mL; 对浓度为1.0×10-6 g/mL的样品进行11次平行测定, 相对标准偏差为1.3%. 将此法用于尿液中左亚叶酸钙的测定, 同时进行回收率实验.     

反相流动注射化学发光法测定黄芩苷

在碱性介质中,K3Fe(CN)6氧化鲁米诺产生化学发光,黄芩苷对该体系化学发光具有强烈的抑制作用。利用该化学发光的抑制体系,结合反相流动注射技术,建立了测定黄酮类药物黄芩苷含量的新方法。在优化的条件下,黄芩苷浓度在1.0×10-8～1.0×10-7和3.0×10-7～4.0×10-6mol/L范围内与化学发光抑制强度ΔI呈良好的线性关系,检出限为1.0×10-9mol/L,对3.0×10-7mol/L的黄芩苷进行平行测定10次,得相对标准偏差(RSD)为1.7%。该方法可应用于银黄口服液中黄芩提取物(黄芩苷计)的含量测定。     

A Sensitive Photoinduced Chemiluminescence Method for the Determination of Riboflavin with Flow Injection Analysis

ABSTRACT

A sensitive chemiluminescence (CL) induced by light for the determination of riboflavin with flow injection analysis is developed. Riboflavin shows a strong enhancement effect on the CL reaction of luminol oxidized by periodate after the photochemical reaction of riboflavin in alkaline solution, and the enhancing effect of riboflavin disappears under dark conditions. Oxygen can further improve the enhancing effect of the riboflavin in this CL system, and the effects were also investigated. And based on this phenomenon, a very sensitive method of various surfactants, β-cyclodextrin and organic solvents on this CL system for the determination of riboflavin was described. The range of the linear response for riboflavin using this method is ca. 2.4×10^?8 ~ 1.0 × 10^?6 mol/L, and the detection limit (S/N = 3) is l.0×10^?8 mol/L. The applicability of the method was demonstrated by the determination of riboflavin in pharmaceutical preparation.     

流动注射化学发光法测定痕量NO_2~-的研究与应用

Based on the principle of the reaction of NO2-with I-and formation of I2 in HCl solution,and the chemiluminescence(CL) reaction between luminol and I2 in an alkaline medium.A fairly sensitive,simple and rapid flow-injection analysis-chemiluminescence method for the determina-tion of trace nitrite with the luminal-I——NO2-coupling luminescence system has been developed.Experiment conditions of flow-injection analysis are optimized.When satisfying the condition that Luminol as 4.0×10-4 mol/L,0.7 % KI,0.04 mol/...     

Determination of phenol by flow-injection with chemiluminescence detection based on the hemin-catalysed luminol-hydrogen peroxide reaction

This study established a novel flow injection (FI) methodology for the determination of phenol in aqueous samples based on luminol chemiluminescence (CL) detection. The method was based on the inhibition that phenol caused on the hemin-catalysed chemiluminescence reaction between luminol and hydrogen peroxide in alkaline solution. Optimum conditions and possible mechanisms have been investigated. The linear range was 2.0×10(-9) to 4.0×10(-7)gmL(-1) for phenol. The proposed method is sensitive with a detection limit of 4.0×10(-10)gmL(-1). The relative standard deviation for 11 measurements was 2.3% for 1.0×10(-7)gmL(-1) phenol. The method was applied for the determination of phenol in waste water samples. The results obtained compared well with those by an official method.     

流动注射后化学发光法测定盐酸阿比朵尔

研究了盐酸阿比朵尔在鲁米诺-K3Fe(CN)6化学发光反应体系中的后化学发光反应. 据此建立了测定盐酸阿比朵尔的流动注射后化学发光分析法. 方法的线性范围为6.0×10-9～1.0×10-7 g/mL, 检出限(3σ)为2×10-9 g/mL. 对4.0×10-8 g/mL的盐酸阿比朵尔溶液连续11次平行测定, RSD=1.3%. 方法已用于盐酸阿比朵尔胶囊及尿样中盐酸阿比朵尔含量的测定. 在对这一后化学发光反应的动力学性质、化学发光光谱、紫外可见吸收光谱以及一些相关问题研究的基础上, 提出了可能的反应机理.     

基于银纳米粒子催化鲁米诺-过氧化氢化学发光体系测定丹参酮ⅡA磺酸钠

碱性条件下,丹参酮ⅡA磺酸钠能够抑制鲁米诺-过氧化氢-银纳米粒子的化学发光,据此建立了测定丹参酮ⅡA磺酸钠的化学发光分析方法。通过优化发光底物浓度、反应介质及其浓度和银纳米粒子浓度等因素,确立了最佳实验条件,并探讨了体系的可能机理。在5.0×10~(-6)~0.08 mol/L浓度范围内,体系的化学发光强度与丹参酮ⅡA磺酸钠浓度的对数值呈线性关系,检出限为9.38×10~(-7)mol/L,检测时间为20 s。该方法灵敏度好,检测范围宽,可简单快速地测定注射液中丹参酮ⅡA磺酸钠的含量,检测结果与高效液相色谱法的测定结果较为一致。     

Direct determination of amino acids by pressurized capillary electrochromatography with chemiluminescence detection

A pressurized CEC (pCEC) coupled with on-column chemiluminescence (CL) detection was developed for direct determination of amino acids, which was based on the principle of an enhanced effect of Cu(II)-amino acid complexes on the CL reaction between luminol and hydrogen peroxide in alkaline solution. The effects of some important factors on pCEC separation and CL intensity were systemically investigated. Baseline separation of amino acids including L-histidine (L-His), L-threonine (L-Thr), and L-tyrosine (L-Tyr) was achieved by using a monolithic column with a mobile phase of 5.0x10(-3) mol/L phosphate buffer at pH 8.0 that contained 25% v/v methanol and 5.0x10(-4) mol/L luminol and 1.0x10(-5) mol/L Cu(II) at an applied voltage of -5 kV. The calibration curves of the analytes by plotting the peak height against corresponding concentration were linear over the range of 3.2x10(-6)-3.2x10(-4) mol/L for L-His, 4.1x10(-6)-4.1x10(-4) mol/L for L-Thr, and 6.0x10(-7)-3.0x10(-4) mol/L for L-Tyr. The LODs for L-His, L-Thr, and L-Tyr were 6.4x10(-7), 8.4x10(-7), and 3.0x10(-7) mol/L (S/N = 2), respectively. The proposed method was applied to the analysis of amino acid injection sample with satisfactory results. Mean recoveries for three amino acids were from 84.3 to 89.6%.     

基于纳米CeO_2增敏鲁米诺化学发光法测定对乙酰氨基酚的研究

基于碱性条件下,CeO_2纳米粒子能够有效增敏鲁米诺-KMnO_4体系的化学发光,并结合流动注射技术建立了一种对乙酰氨基酚测定的新方法。实验研究了影响化学发光检测信号的多种因素,并初步探讨了可能的化学发光机理。在最佳实验条件下,对乙酰氨基酚浓度在1.0×10-7~5.0×10-5mol/L范围内与相对化学发光强度的抑制值呈良好的线性相关,相关系数(r2)为0.996 4,检出限(3σ)为3.3×10-8mol/L。对5.0×10-6mol/L的对乙酰氨基酚溶液平行测定11次,计算得相对标准偏差(RSD)为0.3%。该法用于银翘片中对乙酰氨基酚含量的测定,回收率为98.0%;对尿液的加标回收率为97.9%~98.7%,结果满意。     

Flow—injection Chemiluminescence Sensor for the Determination of Gallic Acid by Immobilizing Luminol and Periodate on Anionexchange Resin

ＩｎｔｒｏｄｕｃｔｉｏｎＧａｌｌｉｃａｃｉｄｅｘｉｓｔｓｉｎｔｈｅｌｅａｖｅｓａｎｄｆｒｕｉｔｓｏｆｍａｎｙｔｙｐｅｓｏｆｐｌａｎｔｓａｎｄｉｓｗｉｄｅｌｙｕｓｅｄｉｎｍｅｄｉｃｉｎｅｆｏｒａｎｔｉ ｏｘｉ ｄａｔｉｏｎａｎｄａｎｔｉｂａｃｔｅｒｉａｌａｃｔｉｖｉｔｙ ,ａｎｔｉｆｌａｍｍａｔｏｒｙａｃｔｉｏｎａｎｄａｎｔｉ ｃａｎｃｅｒａｃｔｉｖｉｔｙ .1 5Ｉｎｓｐｉｔｅｏｆｔｈｅｈｅａｌｔｈｉｍｐｏｒｔａｎｃｅｏｆｇａｌｌｉｃａｃｉｄ ,ｉｔｓｍｅｔａｂｏｌｉｓｍａｎｄｋｉｎｅｔｉｃｓｉｎｔｈｅｈｕ…     

Determination of chromium(III) and total chromium using dual channels on glass chip with chemiluminescence detection

A simple, rapid and sensitive method for the determination of chromium(III) and total chromium using the simple dual T channels on glass chip with negative pressure pumping system and chemiluminescence (CL) detection is presented. The CL reaction was based on luminol oxidation by hydrogen peroxide in basic aqueous solution catalyzed by chromium(III). Total chromium in form of chromium(III) was achieved after chromium(VI) was completely reduced by acidic sodium hydrogen sulfite. Total chromium could then be determined with the same strategy as the chromium(III). The CL reagent was composed of 1.0 × 10⁻⁴ mol/L luminol, 1.0 × 10⁻² mol/L hydrogen peroxide and 0.10 mol/L sodium bromide in 0.050 mol/L carbonate buffer (pH 11.00). The 1.0 × 10⁻² mol/L ethylenediaminetetraacetic acid was added into the sample solution in order to improve the selectivity. Chromium(III) could be detected at a notably concentration of 1.6 × 10⁻¹⁶ mol/L and a linear calibration curve was obtained from 1.0 × 10⁻¹⁵ to 1.0 × 10⁻¹³ mol/L. The sample and CL reagent consumption were only 15 and 20 μL, respectively. The analysis time was less than 1 min per sample with the precision (%R.S.D.) was 4.7%. The proposed method has been applied successfully to the analysis of river water, mineral waters, drinking waters and tap water. Its performance was verified by the analysis of certified total chromium-reference materials and by recovery measurement on spiked synthetic seawater sample.     

Quantification of dibromodimethylhydantoin disinfectants in water by chemiluminescent method.

Quantification of 1,3-dibromo-5,5-dimethylhydantoin (DBDMH) was studied by its chemiluminescence (CL) reaction with luminol in an alkaline medium. The stability of DBDMH, 1,3-dichloro-5,5-dimethylhydantoin (DCDMH) and 1-bromo-3-chloro-5,5-dimethylhydantoin (BCDMH) in water was initially assessed by its CL reaction capability. The results indicated that the hydrolysis process was critically dependent on the types of reagents and their pHs. Capillary electrophoresis (CE) separation with CL detection procedure was applied to the DBDMH solution. It was found that at least 3 species in the aqueous DBDMH solution could oxidize luminol to give luminescence: one of them was confirmed to be hypobromite and the others could be the unhydrolyzed or active oxygen produced in the hydrolysis reaction. Finally, a flow-injection chemiluminescent method was proposed for the determination of DBDMH. The concentration of the analyte showed a linear relationship with the CL intensity in the range of 1.2x10(-10) to 1.0x10(-6) mol dm-3 and the detection limit was as low as 6.2x10(-11) mol dm-3. The relative standard deviation (RSD) is 1.7% (n=9) for 2.8x10(-7) mol dm-3 DBDMH.     

KMnO4-甲醛化学发光体系测定对乙酰氨基酚

基于对乙酰氨基酚在酸性介质下对KMnO4-甲醛化学发光体系强烈的增敏作用,建立了对乙酰氨基酚的流动注射化学发光测定方法。在最佳测试条件下,方法对对乙酰氨基酚的检出限(S/N=3)为1.0×10-10mol/L,线性范围为1.0×10-9～1.0×10-5mol/L对乙酰氨基酚,相关系数R为0.9993;对1.0×10-6mol/L的对乙酰氨基酚溶液平行测定11次,其RSD为1.1%。该方法可应用于药物中对乙酰氨基酚含量的测定。     

Chemiluminescence determination of cefotaxime sodium with flow-injection analysis of cerium (IV)-rhodamine 6G system and its application to the binding study of cefotaxime sodium to protein with on-line microdialysis sampling

A simple, sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of cefotaxime sodium based on the chemiluminescence reaction of cefotaxime sodium with ceric sulfate and rhodamine 6G in nitric acid solution. The concentration of cefotaxime sodium was proportional with the CL intensity in the range of 4×10(-8)-8×10(-6) mol L(-1). The detection limit (signal-to-noise ratio=3) was 1×10(-8) mol L(-1). Coupled to the technique of on-line microdialysis sampling, this method was successfully applied to study cefotaxime sodium-protein interaction. The drug and protein were mixed in different molar ratios in Ringer's solution, pH 7.4, and incubated at 37°C in a water bath. The microdialysis probe was utilized to sample the mixed solution at a perfusion rate of 5 μL min(-1) and the recovery of cefotaxime sodium under experimental condition was 16.2%. The data obtained by the present Microdialysis-Flow Injection Analysis-CL method was analyzed with the Scatchard analysis and Klotz plot. The estimated association constant (K) and the number of the binding sites (n) on one of BSA molecule were 5.94×10(4) M(-1) and 1.29 (Klotz equation), respectively.     

Gold Nanoparticles‐enhanced Chemiluminescence Determination of Fenfluramine

A simple and sensitive chemiluminescence method was developed for the determination of fenfluramine. The chemiluminescence signal arising from the reaction between alkaline luminol and N‐bromosuccinimide was found to be greatly enhanced by fenfluramine in the presence of gold nanoparticles. But fenfluramine alone slightly inhibited the CL signal of N‐bromosuccinimide‐luminol in the absence of gold nanoparticles. The experimental parameters that affected the chemiluminescence signal were thoroughly investigated. Under the optimum experimental conditions, the enhanced chemiluminescence intensity was proportional to the concentration of fenfluramine in the range of 0.005‐1.0 mg/L. The detection limit was 0.9 μg/L fenfluramine with a relative standard deviation of 2.5% for 0.1 mg/L fenfluramine solution (n = 11). The method was applied to the determination of fenfluramine in some weight‐reducing tonics and in spiked human urine. A possible CL reaction mechanism was proposed.     

Inhibition of superoxide dismutase, Vitamin C and glutathione on chemiluminescence produced by luminol and the mixture of sulfite and bisulfite

In a system which consisted of luminol (3-aminophthalhydrazide), cobalt sulfate (CoSO4), alkaline buffer and the mixture of NaSO3 and sodium bisulfite (NaHSO3) (sulfite and bisulfite=3:1, m/m), a strong chemiluminescence (CL) was observed using a BPCL ultra-weak luminometer. The CL signals resulted from 3-aminophthalate (the product of oxidized luminol), and were affected by the buffer pH, buffer medium and the concentrations of luminol, CoSO4 and the NaSO3-NaHSO3 mixture. The observation that the CL intensities were inhibited by superoxide dismutase (SOD), Vitamin C (Vc) and glutathione (GSH) in a dose-dependent manner suggested that superoxide radical (O2*-) was involved in the CL reaction and responsible for oxidation of luminol.     

Flow-injection chemiluminescence determination of polyphenols using luminol-NaIO4-gold nanoparticles system

It was found that gold nanoparticles with different sizes could enhance the chemiluminescence (CL) of the luminol-NaIO4 system in alkaline solution. The most intensive CL signals were obtained with gold nanoparticles in diameter of 4 nm and the CL intensity increased linearly with the concentration of gold nanoparticles. The studies of UV-vis spectra, CL spectra, effects of concentrations of luminol and periodate solution were carried out to explore the CL enhancement mechanism. Catechol, hydroquinone and resorcinol were found to inhibit the CL signals of the luminol-NaIO4 reaction catalyzed by gold nanoparticles, which made it applicable for the determination of these polyphenols. Under the selected experimental conditions, the detection limits (3sigma) were in the range of 2.1 x 10(-9) to 1.0 x 10(-10) g ml(-1), the relative standard deviation (R.S.D., n=11) were in the range of 1.7-2.9%. The method has been successfully applied to the determination of catechol in tap water and synthesized samples with satisfactory results.