Determination of cationic surfactants in water samples by their enhanced resonance light scattering with azoviolet

A simple assay of cationic surfactants in water samples was developed based on the measurements of enhanced resonance light scattering (RLS). At pH 6.09 and ionic strength 0.03 M, the interactions of azoviolet (AV) with cationic surfactants, including zephiramine (Zeph) and cetyl trimethyl ammonium bromide (CTMAB), result in enhanced RLS signals characterized by the peaks of 470.0, 485.0 and 495.0 nm. The enhanced RLS intensity is proportional to the concentration of cationic surfactant of Zeph in the range of 0.2~6.0x10(-6) M, and to that of CTMAB in the range of 0.4~4.8x10(-6 )M. The limit of determination (3 sigma) is 2.1x10(-8) M and 3.8x10(-8) M for the two surfactants, respectively. Determinations of cationic surfactants in synthetic and tap water samples were successfully made with a recovery of 90.5~108.6%.     

Determination of serum albumin in the presence of poly(diallyldimethylammonium chloride) by resonance light scattering technique

By means of the resonance light scattering (RLS) technique, a new method was developed to determine the bovine serum albumin (BSA) and human serum albumin (HSA) by the interaction of serum albumin with poly(diallyldimethylammonium chloride) (PDDA). At Tris-NaOH buffer solution, the RLS intensity of serum albumin at the wavelength 320, 550 and 590 nm was obviously enhanced in the presence of PDDA. The influences of some experimental factors, including incubation time, addition sequence of reagents, pH value, concentration of PDDA and foreign substances, on the enhancement of the RLS intensity were examined. The optimum conditions of the experiment were selected. Under the selected experimental condition, the enhanced RLS intensities were directly proportional to the concentrations in the range of (0.0250-2.75)x10(-6) mol/L for BSA and (0.0235-1.17)x10(-6) mol/L for HSA. The detection limits (S/N=3) were 8.40x10(-9) mol/L for BSA and 7.39x10(-9) mol/L for HSA. The synthetic samples were analysed and the results obtained were satisfactory.     

Determination of bovine serum albumin using resonance light scattering technique with sodium dodecylbenzene sulphonate-cetyltrimethylammonium bromide probe

In this paper, the anionic surfactant sodium dodecylbenzene sulphonate (SDBS) and cationic surfactant cetyltrimethylammonium bromide (CTMAB) were used as resonance light scattering (RLS) probe to determine bovine serum albumin (BSA). Based on the weak RLS intensity of SDBS-CTMAB probe and the enhancement of RLS intensity of BSA in the presence of the probe, a simple assay for BSA was developed. The experimental results showed that the formation of three component complex BSA-SDBS-CTMAB is the main reason for the enhancement of RLS intensity of BSA, in which SDBS as a bridge can interact with both BSA and CTMAB. The effects of pH value, incubation time, concentrations of SDBS and CTMAB on the enhanced RLS intensity of BSA were investigated. Under the optimum conditions, the enhanced RLS intensity is proportional to the concentration of BSA in the range from 2.5 x 10(-8) to 2.0 x 10(-6)mol L(-1). The detection limit is 9.7 x 10(-9) mol L(-1) for BSA. The study of foreign substance effect on the determination of BSA indicated that most of metal ions have little effect on the determination of BSA. The results of assay for BSA in synthetic samples were satisfactory.     

Resonance light scattering technique for the determination of proteins with polymethacrylic acid (PMAA)

As a resonance light scattering (RLS) probe, the polyelectrolyte polymethacrylic acid (PMAA) was applied in this assay. The bovine serum albumin (BSA) and human serum albumin (HSA) were determined by the electrostatic interaction of PMAA and proteins. At pH 3.8 Na(2)HPO(4)-citric acid buffer solution, the RLS intensities of PMAA-BSA (HSA) system were greatly enhanced. The characteristic peaks were appeared at the wavelength 320, 546 and 594 nm. The optimization conditions of the reaction were also examined and selected. Under the selected conditions, the RLS intensities were proportional to the protein concentrations in the range of (0.0200-2.00) x 10(-6) mol/L for BSA and (0.0200-2.40) x 10(-6) mol/L for HSA. The influences of some foreign substances were also examined. The synthetic samples containing proteins and some real samples were analyzed and the results obtained were satisfactory.     

全氟辛烷磺酸与蛋白质体系的共振光散射光谱及其分析应用

研究了牛血清白蛋白(BSA)与全氟辛烷磺酸(PFOS)相互作用的共振光散射(RLS)光谱,建立了PFOS的共振光散射分析方法。 在pH值为4.1的BR缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的BSA通过静电引力和疏水作用形成离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于285.0 nm处,增强的散射信号强度与PFOS浓度在0.2～25.0 μmol/L范围内呈线性关系,据此建立了测定PFOS的光散射分析方法,检出限为20.0 nmol/L。 讨论了体系的最佳反应条件及外来物质的干扰,并探讨了反应机理。 建立的共振光散射法用于环境水样中PFOS的测定,RSD≤4.4%。     

硫代乙酰胺共振光散射方法检测环境水样中痕量汞

在pH=3.29的酸性介质中,硫代乙酰胺(TAA)与二价汞离子发生作用产生以379.0 nm为特征峰的共振光散射(RLS)增强光谱。 在此波长下,二价汞离子的浓度与增强共振光散射强度(ΔIRLS)呈线性关系。 研究了酸度、离子强度、温度、时间以及共存物质的影响,确定了最佳测定条件,据此建立了检测痕量汞的共振光散射分析法,线性范围为0.2~10.0 μmol/L,对汞的检测限为0.02 μmol/L。 方法成功的应用于环境水样中汞离子的检测。     

Multi-spectroscopic method study the interaction of anti-inflammatory drug ketoprofen and calf thymus DNA and its analytical application

Interactions of the anti-inflammatory drug ketoprofen with calf thymus DNA (ctDNA) in aqueous solution have been studied by multi-spectroscopic method including resonance light scattering (RLS) technique, ultraviolet spectra (UV), (1)H NMR, etc. The characteristics of RLS spectra, the effective factors and optimum conditions of the reaction have been unequivocally investigated. Mechanism investigations have shown that ketoprofen can bind to ctDNA by groove binding and form large particles, which resulted in the enhancement of RLS intensity. In Critic acid-Na(2)HPO(4) buffer (pH=6.5), ketoprofen has a maximum peak 451.5 nm and the RLS intensity is remarkably enhanced by trace amount of ctDNA due to the interaction between ketoprofen and ctDNA. The enhancement of RLS signal is directly proportional to the concentration of ctDNA in the range of 1.20×10(-6)-1.0×10(-5) mol/L, and its detection limit (3σ) is 1.33×10(-9) mol/L. The method is simple, rapid, practical and relatively free from interference generated by coexisting substance, and was applied to the determination of trace amounts of nucleic acid in synthetic samples with satisfactory results.     

Resonance light-scattering spectrometric study of interaction between enzyme and MPA-modified CdTe nanoparticles

This paper described a novel assay of enzyme based on the measurement of enhanced resonance light-scattering (RLS) signals resulting from the electrostatic and coordination interaction of functionalized CdTe nanoparticles with enzyme. The CdTe nanoparticles which were modified with 3-mercaptocarboxylic acid (MPA) have abundant carboxylic groups (COOH). So the nanoparticles are water-soluble, stable and biocompatible. At pH 8.3 phosphate buffered saline (PBS), the RLS signals of functionalized nano-CdTe are greatly enhanced by bromelain and papain in the region of 220-800 nm characterized by the peak around 318-314 nm, respectively. The optimization conditions of the reaction were also examined and selected. Under the selected conditions, the enhanced RLS intensity is linearly proportional to the concentration of bromelain and papain. The liner range is (0.09-0.9) x 10(-6)mol/L for bromelain and (0.048-0.702) x 10(-6)mol/L for papain. The influences of some foreign substances were also examined. This method can be applied to the determination of enzyme.     

Bi(Ⅲ)-茜素红-CTMAB体系共振散射光谱法测定环境水样中痕量铋

在pH=4.1的HCl溶液中,Bi(Ⅲ)与茜素红和十六烷基三甲基溴化铵（CTMAB）缔合形成粒径较大的疏水性三元离子缔合物（结合比为n(Bi(Ⅲ))∶n(AR)∶n(CTMAB)=1∶3∶3）,该反应导致共振光散射显著增强,最大散射峰位于364 nm。 研究了反应介质、pH值、共振探针浓度等对散射体系的影响,考察了该散射反应的稳定性及共存物质的影响,并对该三元离子缔合物的反应机理进行了探讨。 在2.5 mL 1.0×10-4 mol/L茜素红和2.0 mL 1.0×10-4 mol/L CTMAB最优试验条件下,Bi(Ⅲ)质量浓度与共振光散射强度ΔI364 nm在0～0.384 mg/L范围内呈良好线性关系,检测限为5.898×10-8 g/L,对含量为0.18 mg/L Bi(Ⅲ)溶液进行11次平行测定,其相对标准偏差为2.2%。 将该方法用于环境水样中痕量铋(质量浓度为0.552～0.831 μg/L)的测定,加标回收率为99.5%～100.2%,测定偏差小于2.7%。 基于Bi(Ⅲ)-AR-CTMAB三元离子缔合物的共振光散射光谱,建立了以茜素红染料为光谱探针的痕量Bi(Ⅲ)共振光散射检测新方法。     

基于银纳米粒子的生成测定痕量甲醛

在碱性溶液中甲醛能还原Ag~+得到黄色银纳米粒子,使体系的共振光散射(RLS)强度增强,从而建立起测量环境中痕量甲醛的RLS新方法. 结果表明,新建方法测定甲醛的浓度线性范围为1.0×10~(-6)～2.0×10~(-5) mol/L,检出限为1.0×10~(-7) mol/L,样品加标测定的回收率为96.26%～103.32%. 并且不同浓度的甲醛还原Ag~+得到黄色银纳米粒子的颜色明显不同,基于此建立了一种可视化半定量测定痕量甲醛的新方法,此方法简便快速、灵敏度高. 用于环境水样、室内空气中甲醛的测定,结果满意.     

溴代十六烷基三甲基铵敏化亮绿-脱氧核糖核酸作用的共振光散射增强研究

首次报道在表面活性剂存在下阳离子染料与脱氧核糖核酸(DNA)作用所导致的共振光散射增强(RLSE)。在pH 10.4 ～11.8 和离子强度低于0.050 mol/L的条件下,亮绿(BG)与DNA作用产生398.0 和 467.2 nm的特征RLSE信号,而溴代十六烷基三甲基铵(CTMAB)进一步敏化该RLSE信号。受CTMAB敏化的RLSE信号与0～1.2 mg/L的ctDNA和fsDNA呈正比,检测限低于5 mg/L。方法成功应用于合成样中DNA的测定。     

Interactions of Janus Green B with double stranded DNA and the determination of DNA based on the measurement of enhanced resonance light scattering

A novel assay of DNA with a sensitivity at the nanogram level is proposed based on the measurement of enhanced resonance light scattering (RLS) signals resulting from the interaction of Janus Green B (JGB) with DNA. At pH 6.37 and ionic strength < 0.20, the RLS signals of JGB were greatly enhanced by DNA in the region of 300-650 nm characterized by three peaks at 416.0, 452.0 and 469.2 nm. The binding properties were examined using a Scatchard plot based on the measurement of the enhanced RLS data at 416.0 nm at a high JGB: DNA molar ratio (R > 2.22), and an aggregation mechanism of JGB in the presence of DNA at the nanogram level is proposed. Linear relationships can be established between the enhanced RLS intensity and DNA concentration in the range 0-3.5 micrograms ml-1 for both calf thymus DNA (ctDNA) and fish sperm DNA (fsDNA) if 2.0 x 10(-5) M JGB is employed. The limits of determination were 8.7 ng ml-1 for ctDNA and 9.9 ng ml-1 for fsDNA, respectively. Synthetic samples were analysed satisfactorily.     

基于与HTPB相互作用共振光散射法测定Hg2+

在酸性介质中,汞(Ⅱ)与过量溴离子反应生成四溴合汞(Ⅱ)阴离子,后者进一步与1-十六烷基三苯基溴化膦((1-Hexadecyl)triphenylphosphonium Bromide,HTPB)通过静电作用形成离子缔合体,引起体系的共振光散射信号显著增强,最大散射波长位于291.0 nm处,增强的散射信号强度与Hg2+浓度在0.04～1.5μmol/L范围内呈线性关系,检测限(3σ)为4.0 nmol/L.讨论了体系的最佳反应条件及外来物质的干扰,同时研究了体系的吸收光谱,并探讨了反应机理.建立的共振光散射法用于环境水样中Hg2+的测定,RSD≤4.42%.     

共振光散射法测定丁胺卡那霉素

研究了以达旦黄（TY）作为共振光散射探针测定市售药品中丁胺卡那霉素（AMK）的测定方法．该方法基于在pH=5．5的Britton—Robinson缓冲溶液中，达旦黄和丁胺卡那霉素结合后有强烈的共振光散射作用．在λ=482nm处，共振光散射强度（△IRLS）最大且光散射的强度与AMK的浓度在0．4～2．4mg·L^-1范围内成正比（相关系数r=0．9986），检出限为8．6×10^-3mg·L^-1．该方法简便、快速、灵敏，对1．0mg·L^-1的AMK溶液平行测定11次，RSD=2．57％．用于市售样品的分析测定，结果满意。     

Cetyltrimethylammonium bromide sensitized resonance light-scattering of nucleic acid--Pyronine B and its analytical application

This is the first report on the determination of nucleic acids with Pyronine B (PB) sensitized by cetyltrimethylammonium bromide (CTMAB) with resonance light-scattering (RLS) technique. Under the experimental conditions (1 x 10(-5) mol l(-1) PB, 1 x 10(-5) mol l(-1) CTMAB, pH 7.4, at room temperature, ionic strength 0.02 mol l(-1) NaCl), the interaction of PB with DNA sensitized by CTMAB results in enhanced RLS signals at 328 and 377 nm in the enhanced regions. It was found that the enhanced RLS intensity at 328 nm was proportional to the concentration of DNA in the suitable ranges. The linear range of this assay is 0.0-1.2 microg ml(-1) for calf thymus, 0.0-0.8 microg ml(-1) for fish sperm DNA (fsDNA), and 0.04-1.4 microg ml(-1) for yeast RNA, respectively. The detection limits (3 sigma) are 6.1 ng ml(-1) for calf thymus DNA (ctDNA), 11.2 ng ml(-1) for fish sperm DNA, and 8.6 ng ml(-1) for yeast RNA, respectively. Six synthetic samples were determined satisfactorily. This method is simple, rapid and the dye is inexpensive and stable.     

基于与Ce3+相互作用共振光散射法测定三磷酸腺苷二钠

在Tris-HCl缓冲溶液中, 三磷酸腺苷二钠(ATP)与铈离子(Ce³⁺)相互作用产生很强的共振光散射(RLS)信号。实验表明,增强的散射信号与ATP的浓度在2.0~24 μmol/L范围内呈线性关系,据此建立了一种测定三磷酸腺苷的共振光散射法,检测限(S/N=3σ)为51.25 nmol/L。研究了共存物质的影响,用于临床三磷酸腺苷注射液的测定, RSD=2.29%。     

盐酸奎宁与全氟辛烷磺酸体系的共振光散射光谱研究及其分析应用

研究了盐酸奎宁(Quinine dihydrochloride,简称Quinine)与全氟辛烷磺酸(perfluorooctane sulfonate,简称PFOS)相互作用的共振光散射(resonance light scattering,RLS)光谱,并建立了PFOS的共振光散射分析方法.在pH值为2.87的Britton-Robinson(BR)缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的盐酸奎宁通过静电引力和疏水作用形成2:1的离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于283nm处,增强的散射信号强度与PFOS浓度在0.10～50.0μmol/L范围内呈线性关系,据此建立了测定PFOS的散射分析方法,检测限为9.88nmol/L.讨论了体系的最佳反应条件及外来物质的干扰,同时研究了体系的吸收光谱及荧光光谱,并探讨了反应机理.本方法用于水样及人体血清样品中PFOS的测定,RSD≤4.2%.     

灿烂甲酚蓝共振光谱散射法测定脱氧核糖核酸

研究了灿烂甲酚蓝与脱氧核糖核酸（DNA）作用的共振光散射光谱,在pH＝10．8－11．5的范围内,DNA的加入导致灿烂甲酚蓝共振光散射的增强,在347nm处,存在一共振光散射增强峰,其强度与DNA的浓度呈线性关系,据此建立了一种测定DNA的共振光谱散射法。该方法的线范围为80－100μg/L,检出限为23．3μg/L.     

青霉素类药物的共振光散射光谱分析

本文研究了青霉素钠-碱性品红缔合物的共振散射光谱,建立了pH =7.5的C-L缓冲溶液中,共振光散射法测定青霉素钠的新方法.线性回归方程△IRu=1.74+5.17C,相关系数R=0.9981,检测限3.16×10-7mol/L.该方法简单快速,灵敏度高,对实际样品进行了测定,得到了令人满意的结果.     

Determination of dysprosium by resonance light scattering technique in the presence of BPMPHD

Dysprosium has been determined by resonance light scattering (RLS) method in the presence of 1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexanedione (BPMPHD) at pH 5.5. The Dy-BPMPHD system has three characteristic peaks at 358, 399 and 450 nm, especially the peak at 358 nm, which is proportional to the concentration of Dy(3+) in the range of 1.0 x 10(-10)-1.0 x 10(-5) mol l(-1). The detection limit (S/N=2) is 5.6 x 10(-12) mol l(-1). Synthetic samples are determined satisfactorily. A new sensitive method for detection of dysprosium has been proposed.