| 基于与Ce3+相互作用共振光散射法测定三磷酸腺苷二钠 |
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| 引用本文： | 谭克俊,马璐,覃明丽.基于与Ce3+相互作用共振光散射法测定三磷酸腺苷二钠[J].应用化学,2010,27(6):737-741. |
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| 作者姓名： | 谭克俊 马璐 覃明丽 |
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| 作者单位： | (发光与实时分析教育部重点实验室，西南大学化学化工学院 重庆 400715) |
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| 基金项目： | 国家自然科学基金，重庆市科学技术委员会资助项目，西南大学基金 |
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| 摘 要： | 在Tris-HCl缓冲溶液中, 三磷酸腺苷二钠(ATP)与铈离子(Ce3+)相互作用产生很强的共振光散射(RLS)信号。实验表明，增强的散射信号与ATP的浓度在2.0~24 μmol/L范围内呈线性关系,据此建立了一种测定三磷酸腺苷的共振光散射法,检测限(S/N=3σ)为51.25 nmol/L。研究了共存物质的影响，用于临床三磷酸腺苷注射液的测定, RSD=2.29%。
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| 关 键 词： | 三磷酸腺苷二钠 铈离子 共振光散射 |
| 收稿时间： | 2009-08-24 |
| 修稿时间： | 2009-10-16 |
Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+ |
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| TAN Ke-Jun,MA Lu,QIN Ming-Li.Determination of Adenosine Disodium Triphosphate by Resonance Light Scattering Technique Based on Its Interaction with Ce3+[J].Chinese Journal of Applied Chemistry,2010,27(6):737-741. |
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| Authors: | TAN Ke-Jun MA Lu QIN Ming-Li |
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| Institution: | (Education Ministry Key Laboratory on Luminescence and Real-Time Analysis,College of ;
Chemistry and Chemical Engineering,Southwest University,Chongqing 400715) |
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| Abstract: | In Tris-HCl buffer solution, a resonance light scattering(RLS) detection method for adenosine disodium triphosphate(ATP) was developed based on RLS signal enhancement due to interactions of ATP with cerium ions in an alkalescent aqueous medium. The enhanced RLS intensity was proportional to ATP content in a range of 2.0~24 μmol/L with a limit of determination(LOD,S/N=3σ) of 51.25 nmol/L. The effects of coexisting substances were investigated. The method has been successfully applied to determining the content of ATP in injection samples with a RSD of 2.29%. |
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| Keywords: | Adenosine disodium triphosphate cerium ions resonance light scattering |
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