共查询到20条相似文献,搜索用时 784 毫秒
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1 引 言二氧化氯是很好的饮用水消毒杀菌剂。美国环保局建议ClO2 处理饮用水的浓度不高于 1mg l。测定水中ClO2 的方法有多种 ,但快速、简便的方法不多见。本文研究了酸性溶液中 ,ClO2 对溴甲酚紫的氧化褪色作用 ,建立了用溴甲酚紫测定水中微量ClO2 的分光光度法 ,其线性范围 0~ 2 .8mg L,检出限 0 .0 1mg L ,相关系数r=0 .995 8,用于水样分析 ,结果令人满意。2 实验部分2 1 仪器与试剂 72 2型分光光度计 (上海分析仪器厂 ) ;ClO2 标准溶液 6mg L ,避光于 0℃温度下保存 ;0 .1 1 5mmol L溴甲酚… 相似文献
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催化光度法测定钒已有报道[1~3],在0.45 mol·L-1磷酸介质及加热条件下,过氧化氢氧化溴甲酚紫褪色反应非常缓慢,而痕量钒对此反应具有较高的催化活性,且在一定浓度范围内,钒量与褪色反应程度呈线性关系,据此可建立测定痕量钒的方法。方法的检出限为1.9×10-6g·L-1,测定范围为0·003~1.8 mg·L-1。方法操作简便,重现性好,用于钢样中痕量钒的测定,结果满意。1试验部分1.1仪器与试剂721型分光光度计LB801-2型超级恒温器钒(Ⅴ)标准溶液:1.000 0 g·L-1,称取偏钒酸铵0.229 7 g溶于热水中,冷却,定容于100 mL容量瓶中。溴甲酚紫(用R表示)溶液:… 相似文献
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催化光度法测定痕量甲醛 总被引:24,自引:2,他引:24
基于磷酸介质中甲醛催化溴酸钾氧化溴甲酚紫的褪色反应 ,建立了测定痕量甲醛的方法。方法的检出限为 1.0× 10 - 5g·L- 1,线性范围为 0 .0 2 0~ 0 .4 8μg·ml- 1,方法简便 ,用于空气中测定痕量甲醛 ,结果满意。 相似文献
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痕量铈的催化动力学光度法测定 总被引:3,自引:0,他引:3
基于pH 9.0的氨水-氯化铵缓冲溶液中,痕量铈(Ⅳ)对溴酸钾氧化溴甲酚紫褪色的指示反应有明显的催化作用。据此建立了痕量铈(IV)的光度测定法,铈量在0~4.0μg·L-1间与△A值间呈线性关系,检出限为0.092μg·L-1。用于测定人发、茶叶和大米样品中痕量铈(IV),结果的相对标准偏差在3.6%~5.4%之间,回收率在96%~103%之间。 相似文献
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An electrochemical investigation on the interaction of acid chrome blue K (ACBK) with protein on the mercury electrode with different electrochemical methods such as cyclic voltammetry and linear sweep voltammetry was reported in this paper. In pH 3.0 Britton-Robinson (B-R) buffer solution, ACBK has an irreversible voltammetric reductive peak at -0.23 V (vs. SCE). The addition of human serum albumin (HSA) into the ACBK solution resulted in the decrease of reductive peak currents without the change of the peak potential and no new peaks appeared on the cyclic voltammogram. In the absence and presence of HSA, the electrochemical parameters such as the formal potential E0, the electrode reaction standard rate constant k(s) and the charge transfer coefficient alpha of the interaction system were calculated and the results showed that there were no significant changes between each other. Thus, the interaction of ACBK with protein forms an electro-inactive supramolecular bio-complex, which induces the decrease of the free concentration of ACBK in the reaction solution, and the decrease of the reductive peak current of ACBK. The binding constant and the binding ratio are calculated as 1.29 x 10(8) and 1:2, respectively, and the interaction mechanism is discussed. Based on the binding reaction, this new electrochemical method is further applied to the determination of HSA with the linear range from 3.0-20.0 mg/L and the linear regression equation as deltaIp"(nA) = 10.08 + 19.90 C (mg/L). This method was further applied to determinate the content of protein in the healthy human serum samples with the results in good agreement with the traditional Coomassie brilliant blue G-250 spectrophotometric method. 相似文献
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A new electrochemical method was proposed for the determination of trace amounts of proteins based on the cupferron (Cup) and cadmium(II) complex [Cup‐Cd(II)] as the voltammetric probe. In the selected pH 6.5 Britton–Robinson (B–R) buffer solution, Cup can interact with Cd(II) to form a stable complex of [Cup‐Cd(II)], which had a sensitive linear sweep voltammetric reductive peak at ?0.654 V (vs. SCE). The addition of human serum albumin (HSA) into [Cup‐Cd(II)] complex solution could greatly decrease the reductive peak current without the change of the reductive peak potential, which indicated that HSA could interact with [Cup‐Cd(II)] complex to form a supramolecular biocomplex. The interaction mechanism was discussed and the decrease of reductive peak current was proportional to the concentration of HSA, which could be further used for the proteins determination. The optimal conditions of the binding reaction and the electrochemical detection were carefully investigated. Under the optimal conditions a new quantitative determination method for different kinds of proteins such as HSA, bovine serum albumin (BSA) and bovine hemoglobin (BHb) etc. was developed. The proposed method was simple, practical and relatively free from the interferences of coexisting substances, and it was further applied to the samples determination with satisfactory results. The binding constant (βs) and the binding number (m) of HSA with [Cup‐Cd(II)] complex were calculated by the voltammetric data with the results as βs=1.12×106 and m=1. 相似文献
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Linear sweep voltammetric determination of protein based on its interaction with Alizarin Red S 总被引:11,自引:0,他引:11
In this paper, the electrochemical behavior of the interaction of Alizarin Red S (ARS) with bovine serum albumin (BSA) was investigated on the hanging mercury drop electrode (HMDE). In the acidic solution (pH 4.2), ARS can be easily reduced on the HMDE and it has a well-defined polarographic wave at −0.29 V (SCE). On addition of BSA or human serum albumin (HAS) into the ARS solution, the reduction peak current of ARS decreases without the movement of the peak potential and the appearance of new peaks. The study shows that a new electrochemically non-active complex is formed via intercalation of ARS with BSA or HSA, which can not be reduced on the Hg electrode. The decrease of reductive peak current of ARS is proportional to BSA and HSA concentration in the range of 2.0–60 and 2.0–40 mg l−1, respectively. The detection limit of BSA and HSA is 1.0-mg l−1. The analytical results of human serum and urine samples by this method were in good agreement with the Coomassie brilliant blue G-250 assay. The binding number and the binding interaction mechanism are also discussed. 相似文献
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In this paper the interaction of chromotrope 2B (Ch2B) with proteins was studied by the electrochemical method. Ch2B is an azo dye and shows irreversible electrochemical responses on the mercury electrode in a pH 3.0 Britton‐Robinson (B‐R) buffer solution. After the addition of human serum albumin (HSA) into the Ch2B solution, an interaction took place, and a supramolecular complex was formed in the mixed solution. The electrochemical parameters of the Ch2B‐HSA interaction system were calculated and compared. The results showed that in the absence and presence of HSA in Ch2B solution, the electrochemical parameters such as the formal potential E0, the electrode reaction standard rate constant ks, etc. showed no significant changes, which indicated that an electro‐inactive supramolecular biocomplex was formed. The free concentration of Ch2B in reaction solution was decreased, and this resulted in the decrease of the peak current. The binding constant and the binding ratio were calculated as 7.85 × 109 and 1:2, respectively, and the interaction mechanism was discussed. Based on the decrease of the peak current, this new electrochemical method was proposed for the determination of HSA in the concentration range of 2.0?25.0 mg/L with the linear regression equation as ΔIp′ (nA) = 50.56C (mg/L) — 6.72 (γ = 0.995). This method was further used to determine other different kinds of proteins, such as bovine serum albumin (BSA), oval albumin, etc‥ The new method was successfully applied to detect the content of albumin in healthy human serum samples with the results in good agreement with the traditional Coomassie Brilliant Blue G‐250 spectrophotometric method. 相似文献
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In this paper, a diazo dye of arsenazo III (AAIII) was selected as a new electrochemical probe for the determination of proteins. In Britton-Robinson (B-R) buffer solution of pH 2.4, AAIII had a sensitive second order derivative linear sweep voltammetric reductive peak at ?0.39 V (vs. SCE). After the addition of human serum albumin (HSA) into AAIII solution, an interaction was taken place in the mixed solution and a biosupramolecular complex was formed, which resulted in the decreased reductive peak currents of AAIII. Based on the observed decrease in peak current, a sensitive electrochemical method was proposed for the determination of different proteins such as HSA, bovine serum albumin (BSA) and bovine hemoglobin (BHb). The optimal conditions for the interaction and the interfering effects of coexisting substances on the detection were investigated. The proposed method was successfully applied to the determination of HSA in synthetic samples with the recoveries in the range of 99.13–100.50%. The stoichiometry of HSA-AAIII biocomplex was calculated by voltammetric data with a binding number of 2 and a binding constant of 7.53 × 109. 相似文献
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Studies on the Interaction of Beryllon Ш with Proteins by Voltammetric Technique and its Application 总被引:1,自引:0,他引:1
A new quantitative determination method of proteins using beryllon Ш by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon Ш can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon Ш has a well-defined voltammetric reduction peak at -0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum. 相似文献
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Studies on the Interaction of Beryllon Ⅲ with Proteins by Voltammetric Technique and its Application 总被引:3,自引:0,他引:3
WeiSUN KuiJIAO XueLiangWANG LuDeLU 《中国化学快报》2003,14(12):1275-1277
A new quantitative determination method of proteins using beryllon Ⅲ by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon Ⅲ can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon Ⅲ has a well-defined voltammetric reduction peak at -0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0-40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum. 相似文献
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The interaction of bromothymol blue(BB) with human serum albumin(HSA) was studied by electrochemical techniques and a sensitive method for proteins assay was developed. When BB interacted with HSA, the voltammetric peak current value of BB decreased linearly with the concentration of HSA in a range of 1.0--40.0 mg/L, and the peak potential shifted negatively. Based on the results, a sensitive assay method for proteins, such as HSA, bovine serum albumin(BSA), and egg albumin etc. was established. This method was further applied to determining the HSA in healthy human blood samples, and the results are not significantly different from those obtained by the classic Coomassie Brilliant Blue G-250 spectrophotometic method. The detecting conditions of this method were optimized and the interaction mechanism was discussed. The results show that the electrochemical parameters(formal potential E^0, standard rate constant of the electrode reaction ks, parameter of kinetic nα) of BB have no obvious changes before and after the interaction, which indicate that BB can interact with HSA, forming an electrochemical non-active complex. The equilibrium constant(βs) and the binding ratio(m) for this complex were calculated. The m is 4 and βs is 1.41 × 10^19. This method is fast, simple, highly sensitive, and has good selectivity, which can be used in clinical measurements. 相似文献
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Based on the interaction of cupferron and lead(II) complex [Cup‐Pb(II)] with double‐stranded DNA (dsDNA) a new voltammetric method for the detection of DNA was described in this paper. In pH 4.0 HAc‐hexamine buffer solution, [Cup‐Pb(II)] complex showed a sensitive second order derivative polarographic reductive peak at ‐0.554 V (vs. SCE). After the addition of dsDNA into [Cup‐Pb(II)] mixture solution the reductive peak current decreased with the positive shift of reductive peak potential, which was the typical characteristic of intercalation mode. Under the optimum conditions, the decrease of reductive peak current was directly proportional to the dsDNA concentration in the range from 1.0 to 25.0 mg/L with the linear regression equation as ΔIp″ (nA) = 129.30 + 62.51 C (mg/L) (n = 13, γ = 0.991). The detection limit of 0.90 mg/L (3σ) and the relative standard derivation (RSD) of 2.43% for 10 parallel determinations of 10.0 mg/L dsDNA were found. The method was successfully applied to synthetic samples with good results, and the stoichiometry of dsDNA with [Cup‐Pb(II)] complex was calculated by the voltammetic data with the binding number as 2 and the binding constant as 2.82 × 109. 相似文献