化学发光分析新进展

对近３年来化学发光分析法的最新进展作了评述。内容包括化学发光在无机、有机及生物活性物质分析中的应用,化学发光与其它分析技术的联用等,含参考文献１１３篇。     

地质样品中金的化学发光测定法

本文使用含十二烷基苯磺酸钠，β－环糊精和丙酮的水溶液作稳定剂，解决了低含量金试液的稳定性问题。同时发现，以此稳定剂作为洗脱剂，可以定量洗脱吸附在泡塑上的金，而且得到的洗脱液可以直接用于金的化学发光测定。在此基础上，建立了一个灵敏，简便，实用的地质样品中痕量金的化学发光测定法。     

流动注射—化学发光法测定苯甲醛

依据苯甲醛增强没食子酸－过氧化氢－氢氧化钠体系化学发光的性质，建立了苯甲醛的流动注射化学发光分析法。     

化学发光分析法在环境分析中的应用

本文评述了１９９３－１９９７年化学发光分析法在环境分析中的应用,引用文献６１篇。     

中国化学发光分析进展

化学发光分析是近年来获得迅速发展的一类高灵敏度的微量及痕量分析技术,应用广泛,前景诱人,是当前分析化学研究中的一个新热点。我国的研究工作虽起步较晚,但发展速度很快,本文就我国学者在这一领域的研究进展作一全面回顾。     

化学发光联用技术在兽药残留分析中的应用进展

本文对近年来化学发光联用技术在兽药残留分析中的应用进展进行了评述,主要包括化学发光免疫分析、分子印迹-化学发光分析、微流控芯片-化学发光检测等,引用文献31篇.     

固体表面化学发光法测定微量样品中钴的研究

固体表面化学发光法测定微量样品中钴的研究章竹君，杨维平，吕九如（陕西师范大学化学系，西安，７１００６２）关键词固体表面发光，化学发光，钴，微量技术固体表面化学发光法（ＳＳＣＬ）已用于多种无机离子及生物活性物质的测定［１］，该法结合多种分离技术能有效地...     

高锰酸钾-甲醛-无机还原剂化学发光体系

在甲醛存在下，高锰酸钾与无机还原剂Fe（Ⅱ），S^2-,I^-,Cu（Ⅰ）等能够发生化学发光反应，产生很强的化学发光，据此利用流动注射技术研究了这些化学发光反应。考察了这些反应应用于铁，硫、碘、铜等元素化学发光测定的可行性，并探讨了反应机理。     

鲁米诺化学发光分析法研究进展

从化学发光反应机理和应用进展两个方面对鲁米诺-过氧化氢、鲁米诺-铁氰化钾、鲁米诺-碘化物、鲁米诺-高锰酸钾和鲁米诺-溶解氧等化学发光体系进行了综述;指出鲁米诺化学发光体系是应用最为广泛的一类化学发光体系,同时对鲁米诺化学发光分析法的发展方向进行了展望.     

固体表面化学发光分析 Ⅰ.不渗透阻挡技术的应用和研究

本文应用不渗透阻挡技术在固体基质上保持恒定的化学发光区域，实现了固体表面化学发光分析，并对固体表面化学发光特性，发光的均匀性和重视性，固体基质的选择和纯化等方面进行了研究，测定了１３种无机及生物活性物质固体表面化学发光的线性范围及检测限。由于此法克服了固体基质荧光分析和固体表面室温磷光分析的散射光背景的影响，获得了更高的信噪比和更好的检测限。     

Analytical applications of photoinduced chemiluminescence in flow systems--a review

In this review, the recent evolution and the state of the art of photochemical reactions coupled with chemiluminescence processes are presented. Different chemiluminescence systems have been considered together with suitable photochemical derivatization processes that can affect either the analyte of interest or even the chemiluminogenic reagent, producing some derivatives able to participate more efficiently in the CL reactions and enhancing the CL emission. The on-line integration of the photochemical reactions as well as the coupling of this resulting photoinduced chemiluminescence (PICL) method with dynamic analytical systems, such as flow injection analysis, liquid or gas chromatography and capillary electrophoresis, have been discussed. Important applications of PICL have been proposed in environmental, pharmaceutical and food analysis.     

化学发光分析法的发展与应用

近几年来,化学发光分析作为一种高灵敏度的痕量分析方法,发展迅速,不断深入到有机、药物、食检、环境和材料科学等各个领域.用于化学发光分析的发光体系也日益增多.介绍了很多近年来初步建立起来的新的化学发光体系,并对其应用作了较为详细的评述.     

Chemiluminescence detection in HPLC and CE for pharmaceutical and biomedical analysis

The present paper reviews the developments and applications of chemiluminescence detection with HPLC and CE in pharmaceutical and biomedical analysis. The chemiluminescence systems, chemiluminogenic reagents and derivatization reagents, improvements in instrumental design as well as their contributions to the practical applications, are all presented. The advantages and limitations of current detection methodology and future prospects for improvement are briefly discussed.     

Chemiluminescence assay for quinones based on generation of reactive oxygen species through the redox cycle of quinone

A sensitive and selective chemiluminescence assay for the determination of quinones was developed. The method was based on generation of reactive oxygen species through the redox reaction between quinone and dithiothreitol as reductant, and then the generated reactive oxygen was detected by luminol chemiluminescence. The chemiluminescence was intense, long-lived, and proportional to quinone concentration. It is concluded that superoxide anion was involved in the proposed chemiluminescence reaction because the chemiluminescence intensity was decreased only in the presence of superoxide dismutase. Among the tested quinones, the chemiluminescence was observed from 9,10-phenanthrenequinone, 1,2-naphthoquinone, and 1,4-naphthoquinone, whereas it was not observed from 9,10-anthraquinone and 1,4-benzoquinone. The chemiluminescence property was greatly different according to the structure of quinones. The chemiluminescence was also observed for biologically important quinones such as ubiquinone. Therefore, a simple and rapid assay for ubiquinone in pharmaceutical preparation was developed based on the proposed chemiluminescence reaction. The detection limit (blank + 3SD) of ubiquinone was 0.05 μM (9 ng/assay) with an analysis time of 30 s per sample. The developed assay allowed the direct determination of ubiquinone in pharmaceutical preparation without any purification procedure. Figure Chemiluminescence generated through the redox cycle of quinone     

Sulfur and nitrogen chemiluminescence detection in gas chromatographic analysis

Sulfur and nitrogen compounds exist in a wide variety of analytical samples, from harmful contaminants in refinery streams to important ingredients in certain beverages, spices and condiments. Accurate determination of sulfur/nitrogen concentrations in these diverse samples is very important for process monitoring, quality control, product development, as well as basic research in these different industries. Due to the complex nature of the matrices, and the fact that the sulfur/nitrogen analytes usually exist at low concentrations, an element-selective detector for sulfur/nitrogen is indispensable to the GC analysis of these samples. To meet this challenge, sulfur and nitrogen chemiluminescence detectors for GC have become one of the most powerful tools available to analytical chemists, thanks to their high sensitivity, selectivity and equimolar detector response. In this brief review, an overview of the chemiluminescence detector operating principles, as well as the detector characteristics is presented. Examples of their applications in petroleum refinery, environmental analysis and food/flavor industry are presented. The two chemiluminescence detectors have also been combined into one convenient package and an example of applications of the simultaneous sulfur/nitrogen chemiluminescence detector is also provided.     

Chemiluminescence from poly(styrene-b-ethylene-co-butylene-b-styrene) (SEBS) block copolymers

A detailed analysis of the chemiluminescence emission (CL) from poly(styrene-b-ethylene-co-butylene-b-styrene), SEBS, was carried out. A phenol-phosphite stabilization system based on Irgafos 168 and Irganox 1330, was studied. The kinetic analysis of the CL profile under nitrogen shows a first-order reaction for the decay of chemiluminescence. The activation energy shows different values as a function of temperature, showing that different reactions are involved in the thermal degradation of the SEBS. The CL decay rate correlates well with the amount of the phosphite, Irgafos 168, and confirms the activity of this stabilizer as radical chain-breaking antioxidant in these copolymers.The isothermal analysis of CL under oxygen allows evaluation of the oxidation state, as well as the efficiency of the antioxidants. Good correlations are found between the CL parameters and concentration of Irgafos 168. Several factors suggest that oxidation begins in the interfacial region. Spectral analysis of the chemiluminescence shows the presence of different types of hydroperoxides.Finally, the characterization of the SEBS copolymers by differential scanning calorimetry reveals an order-disorder transition, assigned to aggregates that behave as paracrystalline regions.     

流动注射化学发光分析研究与应用进展

评述近年来流动注射化学发光分析法的最新进展。总结了流动注射化学发光中重要的化学发光试剂及其相应的新发光体系,概述了流动注射化学发光分析与特定的检测技术、分离技术结合形成的分析体系及其应用。     

Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium.

We developed capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium for the analysis of biopolymers, such as DNA and protein. A peroxyoxalate chemiluminescence reagent of bis(2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. When a migration buffer solution containing carboxylmethylcellulose was used, the flow-type chemiluminescence detection cell was found to give a better resolution than the batch-type one. Fluorescein-labeled adenosine triphosphate of 1.0 x 10(-4) M was examined by means of capillary electrophoresis with absorption (260 nm), fluorescence (ex. 496 nm and em. 517 nm), and chemiluminescence detectors. The chemiluminescence detection showed the highest sensitivity among them; the S/N ratios obtained by absorption, fluorescence, and chemiluminescence detections were 4, 38, and 130, respectively. Fluorescein-labeled DNA was prepared through a polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluorescein isothiocyanate was also separated and detected.