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在线电化学/分离分析方法 总被引:1,自引:0,他引:1
对电化学衍化之后续以分离分析手段的在线联用方法作了 概述,讨论了电化学/质谱,在化学。高效液相色谱/质谱,电质学/质谱/质谱,电化学/高效液相色谱和电化学/气相色谱等的研究与应用,展 望了这个领域的发展前景。 相似文献
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新型固相微萃取膜及其在分析沙土中梯恩梯的应用 总被引:2,自引:1,他引:2
本文用酰胺类化舍物和气相色谱固定液制备了一种新型固相微萃取膜,应用该类固相微萃取膜成功地分离了沙土中炸药梯恩梯,并利用气相色谱/质谱联用技术对分离后的样品进行了分析。 相似文献
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《分析化学》2007,35(8):1024-1024
该书主要介绍了色谱-质谱、色谱-傅立叶变换红外光谱、色谱-原子光谱和色谱-色谱联用技术。该书简述了质谱、傅立叶变换红外光谱、原子光谱和核磁共振仪器的结构、工作原理、以及与色谱联用时对接口的一般要求。该书内容丰富,包括:液相色谱-质谱、毛细管电泳-质谱、色谱-傅立叶变换红外光谱、液相色谱-傅立叶变换红外光谱、薄层色谱-傅立叶变换红外光谱、色谱-原子光谱等联用技术,以及液相色谱-液相色谱、气相色谱-气相色谱、气相色谱-液相色谱等不同的分离模式色谱联用技术的应用实例。该书是《色谱技术丛书》之分册,在第一版基础上作了修改和充实,补充了新近发展的仪器、技术与应用实例。 相似文献
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香芸火绒草精油化学成分研究 总被引:6,自引:0,他引:6
用毛细管气相色谱/质谱、毛细管气相色谱/付里叶红外联用技术对青海产香芸火绒草精油化学成分进行分离。共鉴定出119个化俣物,占全部峰面积的96.8%。 相似文献
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蛋白同化激素类兴奋剂质谱法检测的现状和发展 总被引:2,自引:0,他引:2
对蛋白同化激素类兴奋剂检测的发展和现状进行了评述,除目前常规的色谱/色谱联用选择离子检测方法外,强调指出了高分辨质谱和串联质谱的应用前景。 相似文献
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该文对质谱鉴定技术及其与色谱联用的分析方法(包括直接进样质谱分析、气相色谱质谱联用技术、超临界流体与质谱联用技术和液相色谱质谱联用技术)在甘油三酯分析方面的应用进行了综述,评述了各类分析方法的优缺点,对常用的脂质分析数据库进行了介绍,并对甘油三酯分析方法的发展及应用作了展望. 相似文献
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Perkins等[1]用MALDI/TOF/MS对不同种类蛇毒中神经毒素进行了分析,彭嘉柔等[2,3]对江浙蝮蛇和蛇毒粗组份进行了初步质谱表征.但蛇毒蛋白纯化困难,因此对单一组份的研究较少且不系统.本文以白眉蝮蛇蛇毒(AgkistrodonblomhoffiiUssurensis,ABUV)为原料,纯化得到了精氨酸酯酶(Arginineesterase,AEase)、磷脂酶A2(PhospholipaseA2,PLA2)、纤溶酶(fibrinolyticenzyme)和L-氨基酸氧化酶(L-aminoacidoxidase),并且用MALDI/TOF/MS法对它们和蛇毒粗毒进行了系统研究.1 实验部分1.1 仪器和药品 激光解吸质谱仪为美国Molecular公司L… 相似文献
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Taichi Usui Makoto Ogata Takeomi Murata Kazuhiro Ichikawa Yoshiyuki Sakano Yasunori Nakamura 《Journal of carbohydrate chemistry》2013,32(7-8):421-430
Negative ion Q-TOF MS/MS spectra are shown to be very useful for sequential analysis of the glycosidic linkage in the α-gluco-oligosaccharides (DP 3-6) derived from an amylopectin molecule. The composition of the fragmentation ions generated from these compounds enabled us to distinguish two kinds of glycosidic linkage, α-(1→4) and α-(1→6), at same time to determine the glucose sequence from the reducing end of the oligosaccharide. 相似文献
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采用改进的圆盘凝胶电泳提取人血清中低分子量蛋白质, 去除了血清中分子量大于3×104的蛋白质, 将提取的低分子量蛋白质热变性后直接在溶液中酶解成肽, 经液相色谱-质谱分析, 并进行Mascot数据库检索, 确认出人血清中97种蛋白质. 相似文献
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Lifang Ma Xiting Wang Zhengyan Fu Aimin Sun Bo Gao 《Biomedical chromatography : BMC》2013,27(9):1117-1122
To investigate the consistency and bioequivalence of tacrolimus ointment reference and trial formulation, the tacrolimus concentrations in blood and skin were determined by HPLC‐ESI‐MS/MS following topical application of two kinds of ointment in porcine skin in a parallel, cross‐over trial. The plasma protein of blood was precipitated by acetonitrile and the tacrolimus in skin was extracted by acetonitrile before HPLC‐ESI‐MS/MS analysis. The internal calibration method (diazepam was the internal standard) was used for quantification analysis (R2 > 0.9999), with linear range from 0.05 to 5 ng/mL for blood samples and from 1 to 200 ng/mL for skin samples. The limits of detection for the porcine blood and skin were 0.005 and 0.5 ng/mL, respectively. The average recoveries for the porcine blood and skin spiked at three levels were 97.56–109.53 and 96.48–103.57%, respectively. The precision expressed in RSDs was from 3.43 to 10.83% for porcine blood and from 3.10 to 8.69% for porcine skin. For the same pig, the tacrolimus concentrations and variation with time of the two kinds of ointment in porcine skin were similar, although variation occurred with different individuals. These results showed that the release and penetration of tacrolimus from the reference and trial formulation are similar. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
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GC/MS和ESI/MS/MS同位素内标法检测甲基丙二酸血症 总被引:6,自引:0,他引:6
以甲基丙二酸血症为对象,分别用GC/MS和ESI/MS/MS方法对该疾病进行了定性和定量检测.通过对样品前处理和分离条件的改善,对疾病的标识化合物之一甲基丙二酸进行了定量测定,其稳定性、精密度和回收率结果很好.同时比较了GC/MS和ESI/MS/MS两种方法的特点,发现两种方法的结合不仅可满足新生儿代谢疾病筛查的要求,同时还可对高危人群进行诊断. 相似文献
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Liquid chromatography-mass spectrometry (LC/MS) has become one of the most widely used analytical techniques in both qualitative and quantitative analysis of small molecules. Recently, with the increasing demand for ever-higher sample throughput, the use of faster chromatographic separations has become popular, along with other LC/MS methods that decrease analytical cycle-time. The burgeoning use of LC/MS has meant that the primary expertise of many practitioners today is not in the field of LC/MS, which has been facilitated by the ease-of-use of modern LC/MS systems. An examination of the current state of the literature, relating to "fast LC/MS", should serve well to those new to LC/MS, and should help them in the development of fast LC/MS methods that are effective in terms of both the chromatography and the utilization of the mass spectrometer. This review paper focuses on fast LC/MS analyses of small molecules that have been reported in peer-reviewed publications. 相似文献
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2 氨基 3 硝基吡啶和2 氨基 5 硝基吡啶的一级质谱图接近,单纯通过一级质谱图较难区分这两种异构体,利用离子阱质谱的串联质谱技术对2 氨基 3 硝基吡啶和2 氨基 5 硝基吡啶在离子阱内以He作碰撞气进行碰撞诱导裂解,所得的二级质谱图表明,两者之间存在明显的差别,可用于2 氨基 3 硝基吡啶和2 氨基 5 硝基吡啶的鉴别。 相似文献