毛细管电泳电化学检测法测定红葡萄酒中的多元酚

目前 ,人类各种疾病约 89%起因于活性氧 .因此消除活性氧基团 ,使过氧化物对机体的损伤降到最低限度已成为研究的热点 1] . Maxwell等 2 ] 测试了红葡萄酒在人体血液中的抗氧化能力 .发现从刚喝下红葡萄酒时起 ,抗氧化活性就开始上升 ,90 min后达到最大 ,抗氧化活性平均上升约 1 5 % .葡萄酒中的多酚含量与活性氧消除能力的相关系数高达 0 .9686,所以确立葡萄酒中多元酚的分析方法有重要意义 .红葡萄酒中含有酚酸类、儿茶素类、黄酮类等多酚类化合物 ,通常采用气相色谱3] 、高效液相色谱 4 ] 测定 .毛细管电泳 ( CE)应用于葡萄酒中多…

Determination of Phenolic Compounds in Red Wine by Capillary Zone Electrophoresis with Electrochemical Detection

Asimultaneous determination of (-) epictechin, (+) catechin, gentisic acid, ferulic acid, quercetin and gallic acid in red wine by capillary zone electrophoresis with electrochemical detection(CE ED) was reported. The effects of potential of working electrode, pHand concentration of running buffer, separation voltage and injection time on CE EDwere investigated. Under the optimum conditions, these six analytes could be separated in a 100 mmol/Lborate buffer(pH=924) within₃₀ min. A 300 μm diameter carbon disk electrode has a good response at +085 V( vs . SCE) for all analytes. The responses were linear over two orders of magnitude with detection limit( S/N=3) ranged from 7×10^-8 g/mLto 14×10^-7 g/mLfor all six analytes. This method has been used for the determination of these analytes in red wine without enrichment, and the assay result was satisfactory.