分析测试新方法(197~203)毛细管电泳-间接紫外法用于太子参中的非衍生化氨基酸检测

建立了一种简单、快速测定赖氨酸(Lys)、脯氨酸(Pro)、亮氨酸(Leu)、丙氨酸(Ala)、组氨酸(His)、苏氨酸(Thr)、蛋氨酸(Met)、丝氨酸(Ser)和甘氨酸(Gly)的毛细管电泳-间接紫外检测方法.通过研究缓冲液的种类和浓度、缓冲液的p H等分离条件对被测组分分离度和灵敏度的影响,从而优化了分析条件.实验结果表明,用0.1mol/L氢氧化钠溶液调节14 mmol/L的对氨基苯磺酸至p H为11.2做为运行缓冲液,当分离电压为20 k V时,9组分在12 min内实现了完全分离.实验结果表明,方法能成功用于不同产地太子参药材中9种氨基酸的含量测定.方法重现性良好,迁移时间和峰高的RSD分别小于2.6%和4.5%(n=7).

Capillary Electrophoresis-Indirect UV Method for Detection of Non-Derivatized Amino Acids in Radix Pseudostell

An analytical system for lysine(Lys), proline(Pro), leucine(Leu), alanine (Ala), histidine(His), threonine(Thr), methionine(Met), serine(Ser) and glycine(Gly) is established using the capillary zone electrophoresis indirect UV detection method. The experimental conditions including type, concentration and pH of buffer solution were studied in detail. The optimal experimental conditions were as follows:0.1 mol/L NaOH-14 mmol/L sulfanilic acid solution (pH=11.2) as the buffer solution, 20 kV as the separation voltage. The nine amino acids could be completely separated in 12 min. Furthermore, the content of the nine amino acids in Radix Pseudostell from different sources were successfully determined. The method showed good reproducibility, RSDs of migration time and peak height were less than 2.6% and 4.5% (n = 7), respectively.