毛细管电色谱柱重复性考察及实验条件的选择

成功地研制了７５μｍ内径偶联式和７５，１００μｍ内径非偶联式毛细管电色谱柱。在自制电色谱柱上获得了小于２．１２％的保留时间ＲＳＤ值，考察了ｐＨ值、有机溶剂浓度对保留行为的影响，并用以指导实验条件的选择。比较了两种方式电色谱柱的峰形。

Research on the Reproducibility and Selection of Experimental Conditions of Capillary Electrochromatography

In this work, we prepared the packed columns for capillary electrochromatography (CEC) with 75microm and 100microm i.d., both of coupled mode and uncoupled mode. A mixture of thiourea and other three aromatic compounds was employed to evaluate the efficiency of the CEC columns. RSD values of retention times smaller than 2.12% was obtained on the home-made CEC system. The influence of pH value and the concentration of the organic solvent in the buffer on the retention behavior was investigated, giving a guide to the selection of experimental conditions. High speed analysis can be achieved by using high pH value buffers. Higher concentration of acetonitrile will improve the elution ability, while lower ocncentration of acetonitrile would improve the resolution. Seven kinds of neutral molecules were separated efficiently within 25 minutes. The results from the two modes of CEC columns were compared.