Au掺杂Fe3O4纳米粒子酶传感器的制备及其应用于有机磷农药检测的研究

合成了金掺杂的四氧化三铁纳米粒子(Au-Fe₃O₄), 以壳聚糖为交联剂, 制备了电流型乙酰胆碱酯酶(AChE)生物传感器, 并将其应用于有机磷农药(OPs)的检测. 实验表明, Au-Fe₃O₄纳米粒子具有良好的生物兼容性, 能够有效地促进电子传递, 修饰了Au-Fe₃O₄纳米粒子的酶传感器, 响应速度快, 检测灵敏度高, 稳定性好; 固定在传感器上的乙酰胆碱酯酶有良好的酶动力学响应, 其表观米氏常数( )为10.3 mmol/L. 利用有机磷农药对乙酰胆碱酯酶的抑制作用, 以硫代乙酰胆碱(ATCh)为底物, 对有机磷农药敌敌畏进行了检测, 检测限达到4.0×10^－13 mol/L.

Au-doped Fe3O4 Nanoparticle Immobilized Acetylcholinesterase Sensor for the Detection of Organophosphorus Pesticide

Au-doped magnetic Fe₃O₄ nanoparticles were synthesized and used for fabrication of acetylcholinesterase (AChE) immobilized electrochemical sensor for the detection of organophosphorus pesticides (OP), such as dichlorvos. The degree of inhibition of the AChE enzyme by OP compounds was determined by measuring the electrooxidation current of the thiocholine generated by the AChE catalyzed hydrolysis of acetylthiocholine (ATCh). The use of these mixed nanoparticles was shown to provide both significant enzyme stabilization and low detection limit for the biosensor. A value of 10.3 mmol/L for the apparent Michaelis-Menten constant ( ) was obtained. The biosensor detected as low as 4.0×10^－13 mol/L dichlorvos.