Amperometric determination of 6-mercaptopurine on functionalized multi-wall carbon nanotubes modified electrode by liquid chromatography coupled with microdialysis and its application to pharmacokinetics in rabbit

In this paper, multi-wall carbon nanotubes fuctionalized with carboxylic groups modified electrode (MWNT-COOH CME) was fabricated. This chemically modified electrode (CME) can be used as the working electrode in the liquid chromatography for the determination of 6-mercaptopurine (6-MP). The results indicate that the CME exhibits efficiently electrocatalytic oxidation for 6-MP with relatively high sensitivity, stability and long-life. The peak currents of 6-MP are linear to its concentrations ranging from 4.0×10⁻⁷ to 1.0×10⁻⁴ mol l⁻¹ with the calculated detection limit (S/N=3) of 2.0×10⁻⁷ mol l⁻¹. Coupled with microdialysis, the method has been successfully applied to the pharmacokinetic study of 6-MP in rabbit blood. This method provides a fast, sensible and simple technique for the pharmacokinetic study of 6-MP in vivo.     

Simultaneous Determination of Aromatic Amines by Liquid Chromatography Coupled with Carbon Nanotubes/Poly (3-methylthiophene) Modified Dual-Electrode

Liquid chromatography with amperometric detection (LC-AD) is developed and applied to simultaneously determine five aromatic amines. In the LC-AD, a new carbon nanotubes/poly(3-methylthiophene) modified dual-electrode is fabricated and then used as the working electrode. It is found that this chemically modified electrode (CME) exhibits efficiently electrocatalytic oxidation for aromatic amines with relatively high sensitivity, stability and long-life. Thus, lower detection in LC-AD can be achieved, which are 4.0 × 10^–8 mol L^–1 for aniline, 1.6 ×10^–7 mol L^–1 for 4-nitroaniline, 1.0 × 10^–7 mol L^–1 for 4-chloroaniline, 1.5 × 10^–7 mol L^–1 for 1-naphthylamine, 1.7 × 10^–7 mol L^–1 for 2-bromoaniline. The recoveries of the five analytes are also determined, which range between 0.95 and 1.05 for drinking water, 0.86 and 1.10 for the LiWa River water.     

基于Zn(Q-Ph)2与DCJTB非掺杂型OLED的制备及其电致发光性能研究

合成了有机发光材料2-苯基-8-羟基喹啉锌Zn(Q-Ph)2, 通过1H NMR, UV-Vis及MS等手段对配合物进行了结构表征. 利用该材料与高效的红光染料DCJTB复合制备出全新结构的非掺杂型OLED器件, 其结构为ITO/NPB/DCJTB/Zn(Q-Ph)2/AlQ3/Al. 将DCJTB超薄层的厚度调节到0—2.0 nm范围内, OLED器件的发光色调经历了黄光、红光和橙光的转变, 并且探讨了DCJTB厚度对OLED发光机理以及发光复合区域的影响. 当DCJTB的厚度为0.5 nm时, 获得了稳定的红光发射, 该器件在5.5 V电压下启亮, 在25 V外加电压下发光亮度达到420 cd/m2, 对应的电流密度为250 mA/cm2.     

聚邻苯二胺修饰电极抗坏血酸氧化酶生物传感器的研究

本文报道了聚邻苯二胺／抗坏血酸氧化酶生物传感器，用这种传感器测定人体血清中的抗坏血酸，线性范围在１．０×１０＾－４～２．５×１０＾－７ｍｏｌ／Ｌ之间，响应时间为７ｓ，检测限为１．０×１０＾－８ｍｏｌ／Ｌ。该传感器具有选择性好、灵敏度高和响应时间短等特点。     

Laser Damage Mechanisms of Amorphous Ta2O5 Films at 1064, 532 and 355nm in One-on-One Regime

Ta2O5 films are deposited on fused silica substrates by conventional e-beam evaporation. Surface topography and chemical composition are examined by atomic force microscopy （AFM） and x-ray photoelectron spectroscopy （XPS）. The calculation of electron structures of Ta2O5 and Ta2O5-x is attempted using a first-principle pseudopotential method within the local density approximation. The laser-induced damage threshold （LIDT） is performed at 1064, 532 and 355 nm in 1-on-1 regime, respectively. The results show that the LIDT increases with the wavelength increasing, which is in agreement with the wavelength effect. However, the LIDT results are not consistent with the empirical equation （I（λ）=aλm）, which may be attributed to the intrinsic absorption of Ta2O5 at the wavelengths of 532 or/and 355 nm. Moreover, different damage morphologies are observed when the films are irradiated at different wavelengths. It is concluded that the laser damage at 1064 nm is the defect dominant mechanism and at 355 nm it is the intrinsic absorption dominant mechanism, whereas at 532 nm it is the combined defect and intrinsic absorption dominant mechanism.     

基于Fe3O4@Au复合纳米粒子标记抗体的电化学免疫方法用于水体中大肠杆菌的检测

合成了Fe₃O₄@Au复合纳米粒子作为辣根过氧化酶标记抗体的载体, 并将该复合纳米粒子标记物应用于电化学放大免疫分析. 将电子媒介体硫堇聚合在玻碳电极表面, 以纳米金作为固定大肠杆菌抗体的基底, 通过辣根过氧化酶催化溶液中H₂O₂产生的电流信号来测定大肠杆菌. 实验结果表明, 该方法对水体中大肠杆菌检测的线性范围为50～1×10⁵ cfu/mL, 检出限为20 cfu/mL. 对过富集后的实际水样进行测定, 该法结果表明, 对水体中大肠杆菌的检测灵敏度达到2 cfu/mL.     

离子液体中聚(3-溴噻吩)的电化学合成和电色效应研究

以1-丁基-3-甲基咪唑六氟磷酸盐离子液体[BMIM]PF₆既作为溶剂又作为支持电介质, 通过恒电流、循环伏安等方法制备聚(3-溴噻吩)(PBrT)膜. 采用红外光谱(FT-IR)和扫描电子显微镜(SEM)对PBrT膜的结构和形貌进行表征, 用热重和差热分析法(TG-DTA)研究聚合膜的热稳定性, 并利用紫外-可见光谱(UV-Vis)、计时电流和计时吸收曲线研究该聚合膜电化学和电致变色的特性. 研究结果表明, 与传统方法比较, 在离子液体[BMIM]PF₆中制备的PBrT膜更致密、光滑, 具有良好的氧化还原可逆性和充放电能力, 电活性高, 热稳定性好. 以该方法制备的PBrT膜颜色变化明显, 响应时间快(0.5 s), 同时由于离子液体具有电位窗宽、导电率高、可循环利用等优点, 因此在电化学聚合等方面具有良好的应用前景.     

2-对联苯-8-羟基喹啉锌的合成及其应用于新型白光OLED

合成了一种全新的有机发光材料2-对联苯-8-羟基喹啉锌(Zn[2-(p-biPh)-8-Q-O]₂), 通过¹H NMR, UV-Vis等对配合物的结构进行表征. 利用该材料制备了新型白光有机电致发光器件(OLED), 其结构为: ITO/NPB (N,N'-双(1-萘基)-N,N'-二苯基-1,1'-二苯基-4,4'-二胺)/BCP (2,9-二甲基-4,7-二苯基-1,10-菲咯啉)/Zn[2-(p-biPh)-8-Q-O]₂/Al. 通过调节空穴阻挡层BCP的厚度, 实现了NPB(蓝光发射)和Zn[2-(p-biPh)-8-Q-O]₂(黄光发射)作为器件双发光层的有效复合, 并研究了其发光机理. 当BCP层的厚度为2.0 nm时, 获得了稳定的白色发光; 该器件在6 V电压下启亮, 20 V电压时最大发光亮度达到130 cd/m², 电流效率为0.224 cd/A.     

纳米二氧化锡电极的制备及其用于水体中大肠杆菌的快速计数研究

制备了新型纳米二氧化锡电极, 将此电极用于水体中大肠杆菌的快速计数研究. 实验结果表明, 用该电极为工作电极, 采用计时电流法能简便、快速、灵敏地对水体中的大肠杆菌进行计数. 通过大肠杆菌脂质过氧化后丙二醛含量的检测, 对大肠杆菌在纳米二氧化锡电极上的电化学响应机理进行了初步的探讨.