| Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump |
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| 作者姓名： | 张庆利 王莉 翁羽翔 邱祥冈 王渭池 阎吉祥 |
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| 作者单位： | Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China;Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China;Beijing Institute of Technology, Beijing 100080,China;Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China;National Laboratory for Superconductivity, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China;Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China;Beijing Institute of Technology, Beijing 100080,China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China (Grant No 20373088) and Program for innovation group (Grant No 60321002). |
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| 摘 要： | Elucidating the initial kinetics of folding pathways is critical to the understanding of the protein folding mechanism. Transient infrared spectroscopy has proved a powerful tool to probe the folding kinetics. Herein we report the construction of a nanosecond laser-induced temperature-jump （T-jump） technique coupled to a nanosecond timeresolved transient mid-infrared （mid-IR） spectrometer system capable of investigating the protein folding kinetics with a temporal resolution of 50 ns after deconvolution of the instrumental response function. The mid-IR source is a liquid N2 cooled CO laser covering a spectral range of 5.0μm （2000 cm^-1）-6.5μm （1540 cm^-1）. The heating pulse was generated by a high pressure H2 Raman shifter at wavelength of 1.9μm. The maximum temperature-jump could reach as high as 26±1℃. The fast folding/unfolding dynamics of cytochrome C was investigated by the constructed system, providing an example.
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| 关 键 词： | 微秒 纳秒 激光诱导升温 瞬时红外光谱 动力学 |
| 收稿时间： | 7/6/2005 12:00:00 AM |
| 修稿时间： | 9/8/2005 12:00:00 AM |
Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump |
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| Zhang Qing-Li,Wang Li,Weng Yu-Xiang,Qiu Xiang-Gang,Wang Wei-Chi and Yan Ji-Xiang.Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump[J].Chinese Physics B,2005,14(12):2484-2490. |
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| Authors: | Zhang Qing-Li Wang Li Weng Yu-Xiang Qiu Xiang-Gang Wang Wei-Chi and Yan Ji-Xiang |
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| Institution: | Beijing Institute of Technology, Beijing 100080,China; Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China;Beijing Institute of Technology, Beijing 100080,China; Laboratory of Soft Matter Physics, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China; National Laboratory for Superconductivity, Institute of Physics,Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | Elucidating the initial kinetics of folding pathways is critical to the
understanding of the protein folding mechanism. Transient infrared
spectroscopy has proved a powerful tool to probe the folding kinetics.
Herein we report the construction of a nanosecond laser-induced
temperature-jump ($T$-jump) technique coupled to a nanosecond time-resolved
transient mid-infrared (mid-IR) spectrometer system capable of investigating
the protein folding kinetics with a temporal resolution of 50 ns after
deconvolution of the instrumental response function. The mid-IR source is a
liquid N$_{2}$ cooled CO laser covering a spectral range of 5.0$\mu$m
(2000 cm$^{-1}) \sim $ 6.5$\mu$m (1540 cm$^{-1})$. The heating pulse
was generated by a high pressure H$_{2}$ Raman shifter at wavelength of
1.9$\mu$m. The maximum temperature-jump could reach as high as 26$\pm
$1$^{\circ}$C. The fast folding/unfolding dynamics of cytochrome $C$ was
investigated by the constructed system, providing an example. |
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| Keywords: | protein folding sub-microsecond laser-induced
temperature-jump transient infrared\linebreak
\hbox{\hskip 1 9cm} spectrum dynamics cytochrome $C$ |
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